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@article{1371631, author = {AbdelandMohsen, A.M. and Jančář, Josef and AbdelandRahman, R.M. and Vojtek, Libor and Hyršl, Pavel and Dušková, Monika and Nejezchlebová, Helena}, article_location = {Amsterdam}, article_number = {1-2}, doi = {http://dx.doi.org/10.1016/j.ijpharm.2017.02.003}, keywords = {In-situ silver; hyaluronan; cytotoxicity; wound dressing}, language = {eng}, issn = {0378-5173}, journal = {International Journal of Pharmaceutics}, title = {A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations}, volume = {520}, year = {2017} }
TY - JOUR ID - 1371631 AU - Abdel-Mohsen, A.M. - Jančář, Josef - Abdel-Rahman, R.M. - Vojtek, Libor - Hyršl, Pavel - Dušková, Monika - Nejezchlebová, Helena PY - 2017 TI - A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations JF - International Journal of Pharmaceutics VL - 520 IS - 1-2 SP - 241-253 EP - 241-253 PB - Elsevier Science BV SN - 03785173 KW - In-situ silver KW - hyaluronan KW - cytotoxicity KW - wound dressing N2 - In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2 mg/100 ml) were added at ambient temperature to sodium hyaluronate solution (40 mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA 3 fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25 ± 2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics were evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/Ag-NPs-2 mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering. ER -
ABDEL-MOHSEN, A.M., Josef JANČÁŘ, R.M. ABDEL-RAHMAN, Libor VOJTEK, Pavel HYRŠL, Monika DUŠKOVÁ a Helena NEJEZCHLEBOVÁ. A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations. \textit{International Journal of Pharmaceutics}. Amsterdam: Elsevier Science BV, 2017, roč.~520, 1-2, s.~241-253. ISSN~0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2017.02.003.
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