A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for
Wound crossMark and Chronic Ulcer Dressing: In Vitro and In
Vivo Evaluations

J 2017

A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations

ABDEL-MOHSEN, A.M., Josef JANČÁŘ, R.M. ABDEL-RAHMAN, Libor VOJTEK, Pavel HYRŠL et. al.

Základní údaje

Originální název

A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations

Název česky

A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations

Autoři

ABDEL-MOHSEN, A.M. (818 Egypt, garant), Josef JANČÁŘ (203 Česká republika), R.M. ABDEL-RAHMAN (818 Egypt), Libor VOJTEK (203 Česká republika, domácí), Pavel HYRŠL (203 Česká republika, domácí), Monika DUŠKOVÁ (203 Česká republika, domácí) a Helena NEJEZCHLEBOVÁ (203 Česká republika)

Vydání

International Journal of Pharmaceutics, Amsterdam, Elsevier Science BV, 2017, 0378-5173

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.862

Kód RIV

RIV/00216224:14310/17:00096115

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.ijpharm.2017.02.003

UT WoS

000396948400025

Klíčová slova česky

In-situ stříbro; hyaluronan; cytotoxicita; obvazový materiál

Klíčová slova anglicky

In-situ silver; hyaluronan; cytotoxicity; wound dressing

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 3. 2018 14:47, Ing. Nicole Zrilić

Anotace

V originále

In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2 mg/100 ml) were added at ambient temperature to sodium hyaluronate solution (40 mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA 3 fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25 ± 2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics were evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/Ag-NPs-2 mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.

Citovat

ABDEL-MOHSEN, A.M., Josef JANČÁŘ, R.M. ABDEL-RAHMAN, Libor VOJTEK, Pavel HYRŠL, Monika DUŠKOVÁ a Helena NEJEZCHLEBOVÁ. A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations. International Journal of Pharmaceutics. Amsterdam: Elsevier Science BV, 2017, roč. 520, 1-2, s. 241-253. ISSN 0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2017.02.003.

@article{1371631,
   author = {AbdelandMohsen, A.M. and Jančář, Josef and AbdelandRahman, R.M. and Vojtek, Libor and Hyršl, Pavel and Dušková, Monika and Nejezchlebová, Helena},
   article_location = {Amsterdam},
   article_number = {1-2},
   doi = {http://dx.doi.org/10.1016/j.ijpharm.2017.02.003},
   keywords = {In-situ silver; hyaluronan; cytotoxicity; wound dressing},
   language = {eng},
   issn = {0378-5173},
   journal = {International Journal of Pharmaceutics},
   title = {A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations},
   volume = {520},
   year = {2017}
}

TY  - JOUR
ID  - 1371631
AU  - Abdel-Mohsen, A.M. - Jančář, Josef - Abdel-Rahman, R.M. - Vojtek, Libor - Hyršl, Pavel - Dušková, Monika - Nejezchlebová, Helena
PY  - 2017
TI  - A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations
JF  - International Journal of Pharmaceutics
VL  - 520
IS  - 1-2
SP  - 241-253
EP  - 241-253
PB  - Elsevier Science BV
SN  - 03785173
KW  - In-situ silver
KW  - hyaluronan
KW  - cytotoxicity
KW  - wound dressing
N2  - In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2 mg/100 ml) were added at ambient temperature to sodium hyaluronate solution (40 mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA 3 fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25 ± 2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics were evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/Ag-NPs-2 mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
ER  -

ABDEL-MOHSEN, A.M., Josef JANČÁŘ, R.M. ABDEL-RAHMAN, Libor VOJTEK, Pavel HYRŠL, Monika DUŠKOVÁ a Helena NEJEZCHLEBOVÁ. A novel in situ Silver/Hyaluronan Bio-nanocomposite Fabrics for Wound crossMark and Chronic Ulcer Dressing: In Vitro and In Vivo Evaluations. \textit{International Journal of Pharmaceutics}. Amsterdam: Elsevier Science BV, 2017, roč.~520, 1-2, s.~241-253. ISSN~0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2017.02.003.

Podrobný výpis o publikaci