A scanning electron microscope technique for studying the
sclerites of Cichlidogyrus

J 2015

A scanning electron microscope technique for studying the sclerites of Cichlidogyrus

FANNES, Wouter, Maarten Pieterjan VANHOVE, Tine HUYSE a Giuseppe PALADINI

Základní údaje

Originální název

A scanning electron microscope technique for studying the sclerites of Cichlidogyrus

Autoři

FANNES, Wouter (56 Belgie), Maarten Pieterjan VANHOVE (56 Belgie, garant, domácí), Tine HUYSE (56 Belgie) a Giuseppe PALADINI (826 Velká Británie a Severní Irsko)

Vydání

Parasitology Research, NEW YORK, Springer-Verlag Berlin Heidelberg 2015, 2015, 0932-0113

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.027

Kód RIV

RIV/00216224:14310/15:00088664

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s00436-015-4446-7

UT WoS

000352723200046

Klíčová slova anglicky

Platyhelminthes; Cichlidae; Anchor; MCO; Male apparatus

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 3. 2018 15:46, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

The genus Cichlidogyrus (Monogenea: Ancyrocephalidae) includes more than 90 species, most of which are gill parasites of African cichlid fishes. Cichlidogyrus has been studied extensively in recent years, but scanning electron microscope (SEM) investigations of the isolated hard parts have not yet been undertaken. In this paper, we describe a method for isolating and scanning the sclerites of individual Cichlidogyrus worms. Twenty-year-old, formol-fixed specimens of Cichlidogyrus casuarinus were subjected to proteinase K digestion in order to release the sclerites from the surrounding soft tissues. SEM micrographs of the haptoral sclerites and the male copulatory organ are presented. The ability to digest formol-fixed specimens makes this method a useful tool for the study of historical museum collections.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

FANNES, Wouter, Maarten Pieterjan VANHOVE, Tine HUYSE a Giuseppe PALADINI. A scanning electron microscope technique for studying the sclerites of Cichlidogyrus. Parasitology Research. NEW YORK: Springer-Verlag Berlin Heidelberg 2015, 2015, roč. 114, č. 5, s. 2031-2034. ISSN 0932-0113. Dostupné z: https://dx.doi.org/10.1007/s00436-015-4446-7.

@article{1372035,
   author = {Fannes, Wouter and Vanhove, Maarten Pieterjan and Huyse, Tine and Paladini, Giuseppe},
   article_location = {NEW YORK},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00436-015-4446-7},
   keywords = {Platyhelminthes; Cichlidae; Anchor; MCO; Male apparatus},
   language = {eng},
   issn = {0932-0113},
   journal = {Parasitology Research},
   title = {A scanning electron microscope technique for studying the sclerites of Cichlidogyrus},
   volume = {114},
   year = {2015}
}

TY  - JOUR
ID  - 1372035
AU  - Fannes, Wouter - Vanhove, Maarten Pieterjan - Huyse, Tine - Paladini, Giuseppe
PY  - 2015
TI  - A scanning electron microscope technique for studying the sclerites of Cichlidogyrus
JF  - Parasitology Research
VL  - 114
IS  - 5
SP  - 2031-2034
EP  - 2031-2034
PB  - Springer-Verlag Berlin Heidelberg 2015
SN  - 09320113
KW  - Platyhelminthes
KW  - Cichlidae
KW  - Anchor
KW  - MCO
KW  - Male apparatus
N2  - The genus Cichlidogyrus (Monogenea: Ancyrocephalidae) includes more than 90 species, most of which are gill parasites of African cichlid fishes. Cichlidogyrus has been studied extensively in recent years, but scanning electron microscope (SEM) investigations of the isolated hard parts have not yet been undertaken. In this paper, we describe a method for isolating and scanning the sclerites of individual Cichlidogyrus worms. Twenty-year-old, formol-fixed specimens of Cichlidogyrus casuarinus were subjected to proteinase K digestion in order to release the sclerites from the surrounding soft tissues. SEM micrographs of the haptoral sclerites and the male copulatory organ are presented. The ability to digest formol-fixed specimens makes this method a useful tool for the study of historical museum collections.
ER  -

FANNES, Wouter, Maarten Pieterjan VANHOVE, Tine HUYSE a Giuseppe PALADINI. A scanning electron microscope technique for studying the sclerites of Cichlidogyrus. \textit{Parasitology Research}. NEW YORK: Springer-Verlag Berlin Heidelberg 2015, 2015, roč.~114, č.~5, s.~2031-2034. ISSN~0932-0113. Dostupné z: https://dx.doi.org/10.1007/s00436-015-4446-7.

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