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@article{1372379,
author = {Bláhová, Lucie and Kohoutek, Jiří and Procházková, Tereza and Prudíková, Michaela and Bláha, Luděk},
article_location = {New York},
article_number = {9},
doi = {http://dx.doi.org/10.3168/jds.2016-10926},
keywords = {bovine milk; phytoestrogen; mass spectrometry; equol},
language = {eng},
issn = {0022-0302},
journal = {Journal of Dairy Science},
title = {Phytoestrogens in milk: Overestimations caused by contamination of the hydrolytic enzyme used during sample extraction},
url = {http://www.sciencedirect.com/science/article/pii/S0022030216304143},
volume = {99},
year = {2016}
}
TY - JOUR
ID - 1372379
AU - Bláhová, Lucie - Kohoutek, Jiří - Procházková, Tereza - Prudíková, Michaela - Bláha, Luděk
PY - 2016
TI - Phytoestrogens in milk: Overestimations caused by contamination of the hydrolytic enzyme used during sample extraction
JF - Journal of Dairy Science
VL - 99
IS - 9
SP - 6973-6982
EP - 6973-6982
PB - Elsevier Science Inc.
SN - 00220302
KW - bovine milk
KW - phytoestrogen
KW - mass spectrometry
KW - equol
UR - http://www.sciencedirect.com/science/article/pii/S0022030216304143
L2 - http://www.sciencedirect.com/science/article/pii/S0022030216304143
N2 - Isoflavones are natural phytoestrogens with antioxidant and endocrine-disrupting potencies. Monitoring of their levels is important to ensure the high quality and safety of food, milk, and dairy products. The efficiency and accuracy of phytoestrogen analyses in complex matrices such as milk depend on the extraction procedure, which often uses hydrolysis by means of the beta-glucuronidase/sulfatase enzyme originating from Helix pomatia. The present study reveals that the commercially available hydrolytic enzyme is contaminated by several phytoestrogen isoflavones (genistein, daidzein, formononetin, and biochanin A) and their metabolite equol, as well as flavones (naringenin and apigenin) and coumestrol. We show that the concentrations of daidzein and genistein in the enzyme could have impaired the results of analyses of the main isoflavones in several previously published studies. Of 8 analyzed compounds, only equol was confirmed in the present study and it serves as a reliable marker of phytoestrogens originating from cow feed. Critical reassessment of phytoestrogen concentrations in milk is needed because several previously published studies might have overestimated the concentrations depending on the extraction procedure used.
ER -
BLÁHOVÁ, Lucie, Jiří KOHOUTEK, Tereza PROCHÁZKOVÁ, Michaela PRUDÍKOVÁ a Luděk BLÁHA. Phytoestrogens in milk: Overestimations caused by contamination of the hydrolytic enzyme used during sample extraction. \textit{Journal of Dairy Science}. New York: Elsevier Science Inc., 2016, roč.~99, č.~9, s.~6973-6982. ISSN~0022-0302. Dostupné z: https://dx.doi.org/10.3168/jds.2016-10926.
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