The 9aaTAD Is Exclusive Activation Domain in Gal4

J 2017

The 9aaTAD Is Exclusive Activation Domain in Gal4

PISKÁČEK, Martin, Marek HAVELKA, Martina ŘEZÁČOVÁ a Andrea KNIGHT

Základní údaje

Originální název

The 9aaTAD Is Exclusive Activation Domain in Gal4

Autoři

PISKÁČEK, Martin (40 Rakousko, garant, domácí), Marek HAVELKA (203 Česká republika, domácí), Martina ŘEZÁČOVÁ (203 Česká republika, domácí) a Andrea KNIGHT (203 Česká republika, domácí)

Vydání

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.766

Kód RIV

RIV/00216224:14110/17:00095629

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0169261

UT WoS

000391639100030

Klíčová slova anglicky

P53 TRANSACTIVATION DOMAIN; GALACTOSE PATHWAY ENZYMES; CREB-BINDING-PROTEIN; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTIONAL ACTIVATION; KIX DOMAIN; IN-VIVO; ACIDIC ACTIVATORS; GENE-EXPRESSION; AMINO-ACIDS

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2018 13:39, Soňa Böhmová

Anotace

V originále

The Gal4 protein is a well-known prototypic acidic activator that has multiple activation domains. We have previously identified a new activation domain called the nine amino acid transactivation domain (9aaTAD) in Gal4 protein. The family of the 9aaTAD activators currently comprises over 40 members including p53, MLL, E2A and other members of the Gal4 family; Oaf1 Pip2, Pdr1 and Pdr3. In this study, we revised function of all reported Gal4 activation domains. Surprisingly, we found that beside of the activation domain 9aaTAD none of the previously reported activation domains had considerable transactivation potential and were not involved in the activation of transcription. Our results demonstrated that the 9aaTAD domain is the only decisive activation domain in the Gal4 protein. We found that the artificial peptides included in the original Gal4 constructs were results of an unintended consequence of cloning that were responsible for the artificial transcriptional activity. Importantly, the activation domain 9aaTAD, which is the exclusive activation domain in Gal4, is also the central part of a conserved sequence recognized by the inhibitory protein Gal80. We propose a revision of the Gal4 regulation, in which the activation domain 9aaTAD is directly linked to both activation function and Gal80 mediated inhibition.

Návaznosti

NV15-32935A, projekt VaV

Název: Analýza gamma-delta T lymfocytů reaktivních na nádorové buňky u pacientů s B-buněčnou chronickou lymfocytární leukémií: nový přístup k buněčné terapii

Citovat

PISKÁČEK, Martin, Marek HAVELKA, Martina ŘEZÁČOVÁ a Andrea KNIGHT. The 9aaTAD Is Exclusive Activation Domain in Gal4. Plos one. San Francisco: Public Library of Science, 2017, roč. 12, č. 1, s. 1-13. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0169261.

@article{1372395,
   author = {Piskáček, Martin and Havelka, Marek and Řezáčová, Martina and Knight, Andrea},
   article_location = {San Francisco},
   article_number = {1},
   doi = {http://dx.doi.org/10.1371/journal.pone.0169261},
   keywords = {P53 TRANSACTIVATION DOMAIN; GALACTOSE PATHWAY ENZYMES; CREB-BINDING-PROTEIN; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTIONAL ACTIVATION; KIX DOMAIN; IN-VIVO; ACIDIC ACTIVATORS; GENE-EXPRESSION; AMINO-ACIDS},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {The 9aaTAD Is Exclusive Activation Domain in Gal4},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1372395
AU  - Piskáček, Martin - Havelka, Marek - Řezáčová, Martina - Knight, Andrea
PY  - 2017
TI  - The 9aaTAD Is Exclusive Activation Domain in Gal4
JF  - Plos one
VL  - 12
IS  - 1
SP  - 1-13
EP  - 1-13
PB  - Public Library of Science
SN  - 19326203
KW  - P53 TRANSACTIVATION DOMAIN
KW  - GALACTOSE PATHWAY ENZYMES
KW  - CREB-BINDING-PROTEIN
KW  - SACCHAROMYCES-CEREVISIAE
KW  - TRANSCRIPTIONAL ACTIVATION
KW  - KIX DOMAIN
KW  - IN-VIVO
KW  - ACIDIC ACTIVATORS
KW  - GENE-EXPRESSION
KW  - AMINO-ACIDS
N2  - The Gal4 protein is a well-known prototypic acidic activator that has multiple activation domains. We have previously identified a new activation domain called the nine amino acid transactivation domain (9aaTAD) in Gal4 protein. The family of the 9aaTAD activators currently comprises over 40 members including p53, MLL, E2A and other members of the Gal4 family; Oaf1 Pip2, Pdr1 and Pdr3. In this study, we revised function of all reported Gal4 activation domains. Surprisingly, we found that beside of the activation domain 9aaTAD none of the previously reported activation domains had considerable transactivation potential and were not involved in the activation of transcription. Our results demonstrated that the 9aaTAD domain is the only decisive activation domain in the Gal4 protein. We found that the artificial peptides included in the original Gal4 constructs were results of an unintended consequence of cloning that were responsible for the artificial transcriptional activity. Importantly, the activation domain 9aaTAD, which is the exclusive activation domain in Gal4, is also the central part of a conserved sequence recognized by the inhibitory protein Gal80. We propose a revision of the Gal4 regulation, in which the activation domain 9aaTAD is directly linked to both activation function and Gal80 mediated inhibition.
ER  -

PISKÁČEK, Martin, Marek HAVELKA, Martina ŘEZÁČOVÁ a Andrea KNIGHT. The 9aaTAD Is Exclusive Activation Domain in Gal4. \textit{Plos one}. San Francisco: Public Library of Science, 2017, roč.~12, č.~1, s.~1-13. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0169261.

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