Assessment of Chemical Impact of Invasive Bryozoan Pectinatella
magnifica on the Environment: Cytotoxicity and Antimicrobial
Activity of P. magnifica Extracts

J 2016

Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts

KOLLAR, Peter, Karel SMEJKAL, Hana SALMONOVA, Eva VLKOVA, Olga LEPSOVA-SKACELOVA et. al.

Základní údaje

Originální název

Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts

Autoři

KOLLAR, Peter (703 Slovensko), Karel SMEJKAL (203 Česká republika), Hana SALMONOVA (203 Česká republika), Eva VLKOVA (203 Česká republika), Olga LEPSOVA-SKACELOVA (203 Česká republika), Zuzana BALOUNOVA (203 Česká republika), Josef RAJCHARD (203 Česká republika), Josef CVACKA (203 Česká republika), Libor JAŠA (203 Česká republika, domácí), Pavel BABICA (203 Česká republika, garant, domácí) a Jiří PAZOUREK (203 Česká republika)

Vydání

Molecules, BASEL, Mayer und Muller, 2016, 1420-3049

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10401 Organic chemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.861

Kód RIV

RIV/00216224:14310/16:00093544

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/molecules21111476

UT WoS

000389918200058

Klíčová slova anglicky

Aeromonas; antimicrobial activity; bacteria; Bryozoa; cyanobacteria; invasive species; Pectinatella magnifica; toxicity

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 3. 2017 11:14, Ing. Andrea Mikešková

Anotace

V originále

Pectinatella magnifica, an invasive bryozoan, might significantly affect ecosystem balance due to its massive occurrence in many areas in Europe and other parts of the world. Biological and chemical analyses are needed to get complete information about the impact of the animal on the environment. In this paper, we aimed to evaluate in vitro cytotoxic effects of five extracts prepared from P. magnifica using LDH assay on THP-1 cell line. Antimicrobial activities of extracts against 22 different bacterial strains were tested by microdilution method. Our study showed that all extracts tested, except aqueous portion, demonstrated LD50 values below 100 mu g/mL, which indicates potential toxicity. The water extract of P. magnifica with LD50 value of 250 mu g/mL also shows potentially harmful effects. Also, an environmental risk resulting from the presence and increasing biomass of potentially toxic benthic cyanobacteria in old colonies should not be underestimated. Toxicity of Pectinatella extracts could be partially caused by presence of Aeromonas species in material, since we found members of these genera as most abundant bacteria associated with P. magnifica. Furthermore, P. magnifica seems to be a promising source of certain antimicrobial agents. Its methanolic extract, hexane, and chloroform fractions possessed selective inhibitory effect on some potential pathogens and food spoiling bacteria in the range of MIC 0.5-10 mg/mL. Future effort should be made to isolate and characterize the content compounds derived from P. magnifica, which could help to identify the substance(s) responsible for the toxic effects of P. magnifica extracts.

Návaznosti

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

KOLLAR, Peter, Karel SMEJKAL, Hana SALMONOVA, Eva VLKOVA, Olga LEPSOVA-SKACELOVA, Zuzana BALOUNOVA, Josef RAJCHARD, Josef CVACKA, Libor JAŠA, Pavel BABICA a Jiří PAZOUREK. Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts. Molecules. BASEL: Mayer und Muller, 2016, roč. 21, č. 11, s. "nestrankovano", 14 s. ISSN 1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules21111476.

@article{1372518,
   author = {Kollar, Peter and Smejkal, Karel and Salmonova, Hana and Vlkova, Eva and LepsovaandSkacelova, Olga and Balounova, Zuzana and Rajchard, Josef and Cvacka, Josef and Jaša, Libor and Babica, Pavel and Pazourek, Jiří},
   article_location = {BASEL},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/molecules21111476},
   keywords = {Aeromonas; antimicrobial activity; bacteria; Bryozoa; cyanobacteria; invasive species; Pectinatella magnifica; toxicity},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts},
   url = {http://www.mdpi.com/1420-3049/21/11/1476},
   volume = {21},
   year = {2016}
}

TY  - JOUR
ID  - 1372518
AU  - Kollar, Peter - Smejkal, Karel - Salmonova, Hana - Vlkova, Eva - Lepsova-Skacelova, Olga - Balounova, Zuzana - Rajchard, Josef - Cvacka, Josef - Jaša, Libor - Babica, Pavel - Pazourek, Jiří
PY  - 2016
TI  - Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts
JF  - Molecules
VL  - 21
IS  - 11
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - Mayer und Muller
SN  - 14203049
KW  - Aeromonas
KW  - antimicrobial activity
KW  - bacteria
KW  - Bryozoa
KW  - cyanobacteria
KW  - invasive species
KW  - Pectinatella magnifica
KW  - toxicity
UR  - http://www.mdpi.com/1420-3049/21/11/1476
L2  - http://www.mdpi.com/1420-3049/21/11/1476
N2  - Pectinatella magnifica, an invasive bryozoan, might significantly affect ecosystem balance due to its massive occurrence in many areas in Europe and other parts of the world. Biological and chemical analyses are needed to get complete information about the impact of the animal on the environment. In this paper, we aimed to evaluate in vitro cytotoxic effects of five extracts prepared from P. magnifica using LDH assay on THP-1 cell line. Antimicrobial activities of extracts against 22 different bacterial strains were tested by microdilution method. Our study showed that all extracts tested, except aqueous portion, demonstrated LD50 values below 100 mu g/mL, which indicates potential toxicity. The water extract of P. magnifica with LD50 value of 250 mu g/mL also shows potentially harmful effects. Also, an environmental risk resulting from the presence and increasing biomass of potentially toxic benthic cyanobacteria in old colonies should not be underestimated. Toxicity of Pectinatella extracts could be partially caused by presence of Aeromonas species in material, since we found members of these genera as most abundant bacteria associated with P. magnifica. Furthermore, P. magnifica seems to be a promising source of certain antimicrobial agents. Its methanolic extract, hexane, and chloroform fractions possessed selective inhibitory effect on some potential pathogens and food spoiling bacteria in the range of MIC 0.5-10 mg/mL. Future effort should be made to isolate and characterize the content compounds derived from P. magnifica, which could help to identify the substance(s) responsible for the toxic effects of P. magnifica extracts.
ER  -

KOLLAR, Peter, Karel SMEJKAL, Hana SALMONOVA, Eva VLKOVA, Olga LEPSOVA-SKACELOVA, Zuzana BALOUNOVA, Josef RAJCHARD, Josef CVACKA, Libor JAŠA, Pavel BABICA a Jiří PAZOUREK. Assessment of Chemical Impact of Invasive Bryozoan Pectinatella magnifica on the Environment: Cytotoxicity and Antimicrobial Activity of P. magnifica Extracts. \textit{Molecules}. BASEL: Mayer und Muller, 2016, roč.~21, č.~11, s.~''nestrankovano'', 14 s. ISSN~1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules21111476.

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