Development and application of a novel recombinant Aleuria
aurantia lectin with enhanced core fucose binding for
identification of glycoprotein biomarkers of hepatocellular
carcinoma

J 2016

Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma

NORTON, Pamela, Mary Ann COMUNALE, Harmin HERRERA, Mengjun WANG, Josef HOUSER et. al.

Základní údaje

Originální název

Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma

Autoři

NORTON, Pamela (840 Spojené státy), Mary Ann COMUNALE (840 Spojené státy), Harmin HERRERA (840 Spojené státy), Mengjun WANG (840 Spojené státy), Josef HOUSER (203 Česká republika, domácí), Michaela WIMMEROVÁ (203 Česká republika, garant, domácí), Patrick R ROMANO (840 Spojené státy) a Anand MEHTA (356 Indie)

Vydání

Proteomics, HOBOKEN, Wiley-Blackwell, 2016, 1615-9853

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.041

Kód RIV

RIV/00216224:14740/16:00088672

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/pmic.201600064

UT WoS

000390809000010

Klíčová slova anglicky

Aleuria aurantia lectin; Biomarker; Glycoproteomics; Glycosylation; Hepatocellular carcinoma; Liver cancer

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 3. 2017 13:06, Mgr. Eva Špillingová

Anotace

V originále

The Aleuria aurantia lectin (AAL) derived from orange peel fungus contains five fucose-binding sites that recognizes fucose bound in -1,2, -1,3, -1,4, and -1,6 linkages to N-acetylglucosamine and galactose. Recently, we have created several recombinant AAL (rAAL) proteins that had altered binding affinity to fucose linkages. In this report, we further characterize the binding specificity of one of the mutated lectins, N224Q lectin. This lectin was characterized by lectin Western blotting, surface plasmon resonance, and glycan microarray and shown to have increased binding to fucosylated glycan. Subsequently, we used this lectin to identify secreted fucosylated glycoproteins from a fetal hepatic cell line. Proteomic analysis revealed several glycoproteins secreted by the fetal cell line that were bound by N224Q lectin. These findings were confirmed by subsequent proteomic analysis of human serum from control patients or patients with hepatocellular carcinoma. These represent candidate oncofetal markers for liver cancer.

Návaznosti

GA13-25401S, projekt VaV

Název: Studium proteinů z patogenů zapojených do rozpoznávání hostitelského organismu

Investor: Grantová agentura ČR, Studium proteinu z patogenu zapojených do rozpoznávání hostitelského organismu

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

NORTON, Pamela, Mary Ann COMUNALE, Harmin HERRERA, Mengjun WANG, Josef HOUSER, Michaela WIMMEROVÁ, Patrick R ROMANO a Anand MEHTA. Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma. Proteomics. HOBOKEN: Wiley-Blackwell, 2016, roč. 16, č. 24, s. 3126-3136. ISSN 1615-9853. Dostupné z: https://dx.doi.org/10.1002/pmic.201600064.

@article{1372607,
   author = {Norton, Pamela and Comunale, Mary Ann and Herrera, Harmin and Wang, Mengjun and Houser, Josef and Wimmerová, Michaela and Romano, Patrick R and Mehta, Anand},
   article_location = {HOBOKEN},
   article_number = {24},
   doi = {http://dx.doi.org/10.1002/pmic.201600064},
   keywords = {Aleuria aurantia lectin; Biomarker; Glycoproteomics; Glycosylation; Hepatocellular carcinoma; Liver cancer},
   language = {eng},
   issn = {1615-9853},
   journal = {Proteomics},
   title = {Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/pmic.201600064/abstract},
   volume = {16},
   year = {2016}
}

TY  - JOUR
ID  - 1372607
AU  - Norton, Pamela - Comunale, Mary Ann - Herrera, Harmin - Wang, Mengjun - Houser, Josef - Wimmerová, Michaela - Romano, Patrick R - Mehta, Anand
PY  - 2016
TI  - Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma
JF  - Proteomics
VL  - 16
IS  - 24
SP  - 3126-3136
EP  - 3126-3136
PB  - Wiley-Blackwell
SN  - 16159853
KW  - Aleuria aurantia lectin
KW  - Biomarker
KW  - Glycoproteomics
KW  - Glycosylation
KW  - Hepatocellular carcinoma
KW  - Liver cancer
UR  - http://onlinelibrary.wiley.com/doi/10.1002/pmic.201600064/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/pmic.201600064/abstract
N2  - The Aleuria aurantia lectin (AAL) derived from orange peel fungus contains five fucose-binding sites that recognizes fucose bound in -1,2, -1,3, -1,4, and -1,6 linkages to N-acetylglucosamine and galactose. Recently, we have created several recombinant AAL (rAAL) proteins that had altered binding affinity to fucose linkages. In this report, we further characterize the binding specificity of one of the mutated lectins, N224Q lectin. This lectin was characterized by lectin Western blotting, surface plasmon resonance, and glycan microarray and shown to have increased binding to fucosylated glycan. Subsequently, we used this lectin to identify secreted fucosylated glycoproteins from a fetal hepatic cell line. Proteomic analysis revealed several glycoproteins secreted by the fetal cell line that were bound by N224Q lectin. These findings were confirmed by subsequent proteomic analysis of human serum from control patients or patients with hepatocellular carcinoma. These represent candidate oncofetal markers for liver cancer.
ER  -

NORTON, Pamela, Mary Ann COMUNALE, Harmin HERRERA, Mengjun WANG, Josef HOUSER, Michaela WIMMEROVÁ, Patrick R ROMANO a Anand MEHTA. Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma. \textit{Proteomics}. HOBOKEN: Wiley-Blackwell, 2016, roč.~16, č.~24, s.~3126-3136. ISSN~1615-9853. Dostupné z: https://dx.doi.org/10.1002/pmic.201600064.

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