Scrape Loading/Dye Transfer Assay

C 2016

Scrape Loading/Dye Transfer Assay

BABICA, Pavel, Iva SOVADINOVÁ a Brad L. UPHAM

Základní údaje

Originální název

Scrape Loading/Dye Transfer Assay

Autoři

BABICA, Pavel (203 Česká republika, garant, domácí), Iva SOVADINOVÁ (203 Česká republika, domácí) a Brad L. UPHAM (840 Spojené státy)

Vydání

New York, USA, Gap Junction Protocols, od s. 133-144, 12 s. Methods in Molecular Biology, 2016

Nakladatel

Springer New York

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14310/16:00093583

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-4939-3664-9

DOI

http://dx.doi.org/10.1007/978-1-4939-3664-9

UT WoS

000381766400010

Klíčová slova anglicky

Dye coupling; Dye transfer; Ex vivo assessment; Gap junctional intercellular communication assessment; High throughput; In vitro assay; Incision loading; Lucifer Yellow; Scalpel loading; Scrape loading; Tracers

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2020 12:49, Mgr. Marie Šípková, DiS.

Anotace

V originále

The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.

Návaznosti

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

BABICA, Pavel, Iva SOVADINOVÁ a Brad L. UPHAM. Scrape Loading/Dye Transfer Assay. Online. In Vinken, Mathieu; Johnstone, Scott R. Gap Junction Protocols. New York, USA: Springer New York, 2016, s. 133-144. Methods in Molecular Biology. ISBN 978-1-4939-3664-9. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-3664-9.

@inbook{1372749,
   author = {Babica, Pavel and Sovadinová, Iva and Upham, Brad L.},
   address = {New York, USA},
   booktitle = {Gap Junction Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-4939-3664-9},
   editor = {Vinken, Mathieu; Johnstone, Scott R.},
   keywords = {Dye coupling; Dye transfer; Ex vivo assessment; Gap junctional intercellular communication assessment; High throughput; In vitro assay; Incision loading; Lucifer Yellow; Scalpel loading; Scrape loading; Tracers},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {New York, USA},
   isbn = {978-1-4939-3664-9},
   pages = {133-144},
   publisher = {Springer New York},
   title = {Scrape Loading/Dye Transfer Assay},
   url = {http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9},
   year = {2016}
}

TY  - CHAP
ID  - 1372749
AU  - Babica, Pavel - Sovadinová, Iva - Upham, Brad L.
PY  - 2016
TI  - Scrape Loading/Dye Transfer Assay
VL  - Methods in Molecular Biology
PB  - Springer New York
CY  - New York, USA
SN  - 9781493936649
KW  - Dye coupling
KW  - Dye transfer
KW  - Ex vivo assessment
KW  - Gap junctional intercellular communication assessment
KW  - High throughput
KW  - In vitro assay
KW  - Incision loading
KW  - Lucifer Yellow
KW  - Scalpel loading
KW  - Scrape loading
KW  - Tracers
UR  - http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9
L2  - http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9
N2  - The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.
ER  -

BABICA, Pavel, Iva SOVADINOVÁ a Brad L. UPHAM. Scrape Loading/Dye Transfer Assay. Online. In Vinken, Mathieu; Johnstone, Scott R. \textit{Gap Junction Protocols}. New York, USA: Springer New York, 2016, s.~133-144. Methods in Molecular Biology. ISBN~978-1-4939-3664-9. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-3664-9.

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