Osteogenic potential of the transcription factor c-MYB

J 2017

Osteogenic potential of the transcription factor c-MYB

ORALOVÁ, Veronika, Eva MATALOVÁ, Michael KILLINGER, Lucia KNOPFOVÁ, Jan ŠMARDA et. al.

Základní údaje

Originální název

Osteogenic potential of the transcription factor c-MYB

Autoři

ORALOVÁ, Veronika (203 Česká republika), Eva MATALOVÁ (203 Česká republika), Michael KILLINGER (203 Česká republika, domácí), Lucia KNOPFOVÁ (203 Česká republika, domácí), Jan ŠMARDA (203 Česká republika, domácí) a marcela BUCHTOVÁ (203 Česká republika, garant, domácí)

Vydání

Calcified Tissue International, New York, Springer, 2017, 0171-967X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.293

Kód RIV

RIV/00216224:14310/17:00094639

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s00223-016-0219-2

UT WoS

000395129400011

Klíčová slova anglicky

mineralised matrix; micromass cultures; mouse limbs; osteogenesis; PCR Array

Štítky

AKR, NZ, rivok

Změněno: 10. 4. 2018 13:01, Ing. Nicole Zrilić

Anotace

V originále

Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in cmyb-overexpressing cultures, whereas downregulation of cmyb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

Citovat

ORALOVÁ, Veronika, Eva MATALOVÁ, Michael KILLINGER, Lucia KNOPFOVÁ, Jan ŠMARDA a marcela BUCHTOVÁ. Osteogenic potential of the transcription factor c-MYB. Calcified Tissue International. New York: Springer, 2017, roč. 100, č. 3, s. 311-322. ISSN 0171-967X. Dostupné z: https://dx.doi.org/10.1007/s00223-016-0219-2.

@article{1372843,
   author = {Oralová, Veronika and Matalová, Eva and Killinger, Michael and Knopfová, Lucia and Šmarda, Jan and Buchtová, marcela},
   article_location = {New York},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00223-016-0219-2},
   keywords = {mineralised matrix; micromass cultures; mouse limbs; osteogenesis; PCR Array},
   language = {eng},
   issn = {0171-967X},
   journal = {Calcified Tissue International},
   title = {Osteogenic potential of the transcription factor c-MYB},
   volume = {100},
   year = {2017}
}

TY  - JOUR
ID  - 1372843
AU  - Oralová, Veronika - Matalová, Eva - Killinger, Michael - Knopfová, Lucia - Šmarda, Jan - Buchtová, marcela
PY  - 2017
TI  - Osteogenic potential of the transcription factor c-MYB
JF  - Calcified Tissue International
VL  - 100
IS  - 3
SP  - 311-322
EP  - 311-322
PB  - Springer
SN  - 0171967X
KW  - mineralised matrix
KW  - micromass cultures
KW  - mouse limbs
KW  - osteogenesis
KW  - PCR Array
N2  - Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in cmyb-overexpressing cultures, whereas downregulation of cmyb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.
ER  -

ORALOVÁ, Veronika, Eva MATALOVÁ, Michael KILLINGER, Lucia KNOPFOVÁ, Jan ŠMARDA a marcela BUCHTOVÁ. Osteogenic potential of the transcription factor c-MYB. \textit{Calcified Tissue International}. New York: Springer, 2017, roč.~100, č.~3, s.~311-322. ISSN~0171-967X. Dostupné z: https://dx.doi.org/10.1007/s00223-016-0219-2.

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