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@article{1372843, author = {Oralová, Veronika and Matalová, Eva and Killinger, Michael and Knopfová, Lucia and Šmarda, Jan and Buchtová, marcela}, article_location = {New York}, article_number = {3}, doi = {http://dx.doi.org/10.1007/s00223-016-0219-2}, keywords = {mineralised matrix; micromass cultures; mouse limbs; osteogenesis; PCR Array}, language = {eng}, issn = {0171-967X}, journal = {Calcified Tissue International}, title = {Osteogenic potential of the transcription factor c-MYB}, volume = {100}, year = {2017} }
TY - JOUR ID - 1372843 AU - Oralová, Veronika - Matalová, Eva - Killinger, Michael - Knopfová, Lucia - Šmarda, Jan - Buchtová, marcela PY - 2017 TI - Osteogenic potential of the transcription factor c-MYB JF - Calcified Tissue International VL - 100 IS - 3 SP - 311-322 EP - 311-322 PB - Springer SN - 0171967X KW - mineralised matrix KW - micromass cultures KW - mouse limbs KW - osteogenesis KW - PCR Array N2 - Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in cmyb-overexpressing cultures, whereas downregulation of cmyb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration. ER -
ORALOVÁ, Veronika, Eva MATALOVÁ, Michael KILLINGER, Lucia KNOPFOVÁ, Jan ŠMARDA a marcela BUCHTOVÁ. Osteogenic potential of the transcription factor c-MYB. \textit{Calcified Tissue International}. New York: Springer, 2017, roč.~100, č.~3, s.~311-322. ISSN~0171-967X. Dostupné z: https://dx.doi.org/10.1007/s00223-016-0219-2.
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