Redox Processes of Guanine Moieties in DNA Heptamers Related to Hydrogen Evolution

TŘÍSKOVÁ, Iveta, Radovan FIALA and Libuše TRNKOVÁ. Redox Processes of Guanine Moieties in DNA Heptamers Related to Hydrogen Evolution. Electroanalysis. 2016, vol. 28, No 11, p. 2841-2848. ISSN 1040-0397. Available from: https://dx.doi.org/10.1002/elan.201600321.

Basic information

• Original name: Redox Processes of Guanine Moieties in DNA Heptamers Related to Hydrogen Evolution
• Authors: TŘÍSKOVÁ, Iveta (203 Czech Republic, belonging to the institution), Radovan FIALA (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Electroanalysis, 2016, 1040-0397.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10403 Physical chemistry
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.851
• RIV identification code: RIV/00216224:14310/16:00093647
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1002/elan.201600321
• UT WoS: 000387891400028
• Keywords (in Czech): vlásenky; NMR DNA heptamerů; cyklická voltametrie; vylučování vodíku; potenciál bodu obratu; neurodegenerativní onemocnění
• Keywords in English: Hairpins; NMR of DNA heptamers; cyclic voltammetry; hydrogen evolution reaction; vertex potential; neurodegenerative diseases
• Tags: AKR, rivok

Abstract

The paper is focused on the electrochemical investigation of DNA heptamers d(GCXXXGC) with different central triplets of nucleotides (XXX=GAA, AAA, CCC, GGG). On a hanging mercury electrode (HMDE), the reduction peaks of adenine (A) and cytosine (C) moieties are detectable, but those of guanine (G) moieties are covered by an intensive current of hydrogen evolution. Because cyclic voltammetry allows us to monitor only the oxidation processes of the G reduced species, the effect of hydrogen evolution reaction (HER) was studied by means of these anodic signals. For different vertex potentials (from -1.60V to -1.85V) and a different number of cycles (1-5), all heptamers in buffered solutions (pH5.8) were tested and double-peak G oxidation signals (GI and GII) evaluated. The different structures of the heptamers in solutions are confirmed by NMR spectra. Both the impact of hydrogen evolution into the production of 7,8-dihydroguanine moieties forming together with hydrogen in negative potentials and the catalytic effect of heptamers on hydrogen evolution are discussed.