Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones

MANDÁKOVÁ, Terezie and Martin LYSÁK. Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones. Current protocols in plant biology. John Wiley & Sons, 2016, vol. 4, No 2, p. 359-371. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20022.

Basic information

• Original name: Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones
• Authors: MANDÁKOVÁ, Terezie (203 Czech Republic, belonging to the institution) and Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Current protocols in plant biology, John Wiley & Sons, 2016, 2379-8068.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/16:00093748
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/cppb.20022
• Keywords in English: BAC FISH; Brassicaceae; chromosome painting; fluorescence in situ hybridization (FISH); nick translation; pachytene chromosomes
• Tags: rivok

Abstract

Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in plants, most often, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the species of origin or a related species are used as painting probes. CP enables visualization and tracing of particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as the corresponding interphase chromosome territories. CCP enables identification of large-scale homeologous chromosome regions and chromosomes shared among two or more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of Arabidopsis chromosome-specific BAC contigs is described.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR