Chromosome Preparation for Cytogenetic Analyses in Arabidopsis

MANDÁKOVÁ, Terezie and Martin LYSÁK. Chromosome Preparation for Cytogenetic Analyses in Arabidopsis. Current protocols in plant biology. John Wiley & Sons, 2016, vol. 1, No 1, p. 43-51. ISSN 2379-8068. Available from: https://dx.doi.org/10.1002/cppb.20009.

Basic information

• Original name: Chromosome Preparation for Cytogenetic Analyses in Arabidopsis
• Authors: MANDÁKOVÁ, Terezie (203 Czech Republic, belonging to the institution) and Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Current protocols in plant biology, John Wiley & Sons, 2016, 2379-8068.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/16:00088725
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/cppb.20009
• Keywords in English: arabidopsis; Brassicaceae; mitotic and meiotic chromosomes; pachytene
• Tags: rivok

Abstract

Despite its small nuclear genome and minute chromosomes, the model plant Arabidopsis thaliana has become a well established model in the field of plant cytogenetics. Since 2000, most cytogenetic and epigenetic approaches have been developed for this species and its congeners. In this chapter, we describe two step-by-step protocols for preparing chromosome preparations from root tip meristems and generative organ tissues of A. thaliana. Although both protocols have been optimized for A. thaliana, the detailed procedures are equally applicable to other members of the genus Arabidopsis as well as other crucifer species (Brassicaceae).

Links

• GBP501/12/G090, research and development project: Name: Evoluce a funkce komplexních genomů rostlin