How diploidization turned a tetraploid into a pseudotriploid

J 2016

How diploidization turned a tetraploid into a pseudotriploid

MANDÁKOVÁ, Terezie, Andrew D. GLOSS, Noah K. WHITEMAN a Martin LYSÁK

Základní údaje

Originální název

How diploidization turned a tetraploid into a pseudotriploid

Autoři

MANDÁKOVÁ, Terezie (203 Česká republika, domácí), Andrew D. GLOSS (840 Spojené státy), Noah K. WHITEMAN (840 Spojené státy) a Martin LYSÁK (203 Česká republika, garant, domácí)

Vydání

American Journal of Botany, St Louis, Botanical Soc Amer Inc, 2016, 0002-9122

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.050

Kód RIV

RIV/00216224:14740/16:00088729

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3732/ajb.1500452

UT WoS

000380821900005

Klíčová slova anglicky

Brassicaceae; centromere loss; chromosome fusion; chromosome translocation; diploidization; dysploidy; karyotype evolution; polyploidy; whole-genome duplication

Štítky

rivok

Změněno: 1. 3. 2017 13:57, Mgr. Eva Špillingová

Anotace

V originále

PREMISE OF THE STUDY: Despite being highly fertile and occupying a large geographic region, the North American heartleaf bittercress (Cardamine cordifolia; Brassicaceae) has a puzzling triploid- like chromosome number (2n = 3x = 24). As most triploids are sterile, we embarked on a detailed analysis of the C. cordifolia genome to elucidate its origin and structure. METHODS: Mitotic and meiotic chromosome complement of C. cordifolia was analyzed by comparative chromosome painting using chromosome-specific BAC contigs of Arabidopsis thaliana. Resulting chromosome patterns were documented by multicolor fluorescence microscopy and compared with known ancestral and extant Brassicaceae genomes. KEY RESULTS: We discovered that C. cordifolia is not a triploid hybrid but a diploidized tetraploid with the prevalence of regular, diploid-like meiotic pairing. The ancestral tetraploid chromosome number (2n = 32) was reduced to a triploid- like number (2n = 24) through four terminal chromosome translocations. CONCLUSIONS: The structure of the pseudotriploid C. cordifolia genome results from a stepwise diploidization process after whole-genome duplication. We showed that translocation-based descending dysploidy (from n = 16 to n = 12) was mediated by the formation of five new chromosomes. The genome of C. cordifolia represents the diploidization process in statu nascendi and provides valuable insights into mechanisms of postpolyploidy rediploidization in land plants. Our data further suggest that chromosome number alone does not need to be a reliable proxy of species' evolutionary past and that the same chromosome number may originate either by polyploidization (hybridization) or due to descending dysploidy.

Návaznosti

GAP501/10/1014, projekt VaV

Název: Evoluce genomu alopolyploidních řeřišnic (Cardamine) rozdílného fylogenetického stáří

Investor: Grantová agentura ČR, Evoluce genomu alopolyploidních řeřišnic (Cardamine) rozdílného fylogenetického stáří

Citovat

MANDÁKOVÁ, Terezie, Andrew D. GLOSS, Noah K. WHITEMAN a Martin LYSÁK. How diploidization turned a tetraploid into a pseudotriploid. American Journal of Botany. St Louis: Botanical Soc Amer Inc, 2016, roč. 103, č. 7, s. 1187-1196. ISSN 0002-9122. Dostupné z: https://dx.doi.org/10.3732/ajb.1500452.

@article{1374270,
   author = {Mandáková, Terezie and Gloss, Andrew D. and Whiteman, Noah K. and Lysák, Martin},
   article_location = {St Louis},
   article_number = {7},
   doi = {http://dx.doi.org/10.3732/ajb.1500452},
   keywords = {Brassicaceae; centromere loss; chromosome fusion; chromosome translocation; diploidization; dysploidy; karyotype evolution; polyploidy; whole-genome duplication},
   language = {eng},
   issn = {0002-9122},
   journal = {American Journal of Botany},
   title = {How diploidization turned a tetraploid into a pseudotriploid},
   url = {http://www.amjbot.org/content/103/7/1187},
   volume = {103},
   year = {2016}
}

TY  - JOUR
ID  - 1374270
AU  - Mandáková, Terezie - Gloss, Andrew D. - Whiteman, Noah K. - Lysák, Martin
PY  - 2016
TI  - How diploidization turned a tetraploid into a pseudotriploid
JF  - American Journal of Botany
VL  - 103
IS  - 7
SP  - 1187-1196
EP  - 1187-1196
PB  - Botanical Soc Amer Inc
SN  - 00029122
KW  - Brassicaceae
KW  - centromere loss
KW  - chromosome fusion
KW  - chromosome translocation
KW  - diploidization
KW  - dysploidy
KW  - karyotype evolution
KW  - polyploidy
KW  - whole-genome duplication
UR  - http://www.amjbot.org/content/103/7/1187
L2  - http://www.amjbot.org/content/103/7/1187
N2  - PREMISE OF THE STUDY: Despite being highly fertile and occupying a large geographic region, the North American heartleaf bittercress (Cardamine cordifolia; Brassicaceae) has a puzzling triploid- like chromosome number (2n = 3x = 24). As most triploids are sterile, we embarked on a detailed analysis of the C. cordifolia genome to elucidate its origin and structure. METHODS: Mitotic and meiotic chromosome complement of C. cordifolia was analyzed by comparative chromosome painting using chromosome-specific BAC contigs of Arabidopsis thaliana. Resulting chromosome patterns were documented by multicolor fluorescence microscopy and compared with known ancestral and extant Brassicaceae genomes. KEY RESULTS: We discovered that C. cordifolia is not a triploid hybrid but a diploidized tetraploid with the prevalence of regular, diploid-like meiotic pairing. The ancestral tetraploid chromosome number (2n = 32) was reduced to a triploid- like number (2n = 24) through four terminal chromosome translocations. CONCLUSIONS: The structure of the pseudotriploid C. cordifolia genome results from a stepwise diploidization process after whole-genome duplication. We showed that translocation-based descending dysploidy (from n = 16 to n = 12) was mediated by the formation of five new chromosomes. The genome of C. cordifolia represents the diploidization process in statu nascendi and provides valuable insights into mechanisms of postpolyploidy rediploidization in land plants. Our data further suggest that chromosome number alone does not need to be a reliable proxy of species' evolutionary past and that the same chromosome number may originate either by polyploidization (hybridization) or due to descending dysploidy.
ER  -

MANDÁKOVÁ, Terezie, Andrew D. GLOSS, Noah K. WHITEMAN a Martin LYSÁK. How diploidization turned a tetraploid into a pseudotriploid. \textit{American Journal of Botany}. St Louis: Botanical Soc Amer Inc, 2016, roč.~103, č.~7, s.~1187-1196. ISSN~0002-9122. Dostupné z: https://dx.doi.org/10.3732/ajb.1500452.

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