MANDÁKOVÁ, Terezie, Andrew D. GLOSS, Noah K. WHITEMAN a Martin LYSÁK. How diploidization turned a tetraploid into a pseudotriploid. American Journal of Botany. St Louis: Botanical Soc Amer Inc, 2016, roč. 103, č. 7, s. 1187-1196. ISSN 0002-9122. Dostupné z: https://dx.doi.org/10.3732/ajb.1500452. |
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@article{1374270, author = {Mandáková, Terezie and Gloss, Andrew D. and Whiteman, Noah K. and Lysák, Martin}, article_location = {St Louis}, article_number = {7}, doi = {http://dx.doi.org/10.3732/ajb.1500452}, keywords = {Brassicaceae; centromere loss; chromosome fusion; chromosome translocation; diploidization; dysploidy; karyotype evolution; polyploidy; whole-genome duplication}, language = {eng}, issn = {0002-9122}, journal = {American Journal of Botany}, title = {How diploidization turned a tetraploid into a pseudotriploid}, url = {http://www.amjbot.org/content/103/7/1187}, volume = {103}, year = {2016} }
TY - JOUR ID - 1374270 AU - Mandáková, Terezie - Gloss, Andrew D. - Whiteman, Noah K. - Lysák, Martin PY - 2016 TI - How diploidization turned a tetraploid into a pseudotriploid JF - American Journal of Botany VL - 103 IS - 7 SP - 1187-1196 EP - 1187-1196 PB - Botanical Soc Amer Inc SN - 00029122 KW - Brassicaceae KW - centromere loss KW - chromosome fusion KW - chromosome translocation KW - diploidization KW - dysploidy KW - karyotype evolution KW - polyploidy KW - whole-genome duplication UR - http://www.amjbot.org/content/103/7/1187 L2 - http://www.amjbot.org/content/103/7/1187 N2 - PREMISE OF THE STUDY: Despite being highly fertile and occupying a large geographic region, the North American heartleaf bittercress (Cardamine cordifolia; Brassicaceae) has a puzzling triploid- like chromosome number (2n = 3x = 24). As most triploids are sterile, we embarked on a detailed analysis of the C. cordifolia genome to elucidate its origin and structure. METHODS: Mitotic and meiotic chromosome complement of C. cordifolia was analyzed by comparative chromosome painting using chromosome-specific BAC contigs of Arabidopsis thaliana. Resulting chromosome patterns were documented by multicolor fluorescence microscopy and compared with known ancestral and extant Brassicaceae genomes. KEY RESULTS: We discovered that C. cordifolia is not a triploid hybrid but a diploidized tetraploid with the prevalence of regular, diploid-like meiotic pairing. The ancestral tetraploid chromosome number (2n = 32) was reduced to a triploid- like number (2n = 24) through four terminal chromosome translocations. CONCLUSIONS: The structure of the pseudotriploid C. cordifolia genome results from a stepwise diploidization process after whole-genome duplication. We showed that translocation-based descending dysploidy (from n = 16 to n = 12) was mediated by the formation of five new chromosomes. The genome of C. cordifolia represents the diploidization process in statu nascendi and provides valuable insights into mechanisms of postpolyploidy rediploidization in land plants. Our data further suggest that chromosome number alone does not need to be a reliable proxy of species' evolutionary past and that the same chromosome number may originate either by polyploidization (hybridization) or due to descending dysploidy. ER -
MANDÁKOVÁ, Terezie, Andrew D. GLOSS, Noah K. WHITEMAN a Martin LYSÁK. How diploidization turned a tetraploid into a pseudotriploid. \textit{American Journal of Botany}. St Louis: Botanical Soc Amer Inc, 2016, roč.~103, č.~7, s.~1187-1196. ISSN~0002-9122. Dostupné z: https://dx.doi.org/10.3732/ajb.1500452.
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