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@article{1375043,
author = {Makhneva, Ekaterina and Manakhov, Anton and Skládal, Petr and Zajíčková, Lenka},
article_location = {LAUSANNE},
article_number = {March},
doi = {http://dx.doi.org/10.1016/j.surfcoat.2015.09.035},
keywords = {Plasma polymerization; Cyclopropylamine; Stable amine-rich films; Quartz crystal microbalance; Immunosensor; Label-free detection},
language = {eng},
issn = {0257-8972},
journal = {SURFACE & COATINGS TECHNOLOGY},
title = {Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions},
url = {http://www.sciencedirect.com/science/article/pii/S0257897215302747},
volume = {290},
year = {2016}
}
TY - JOUR
ID - 1375043
AU - Makhneva, Ekaterina - Manakhov, Anton - Skládal, Petr - Zajíčková, Lenka
PY - 2016
TI - Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions
JF - SURFACE & COATINGS TECHNOLOGY
VL - 290
IS - March
SP - 116-123
EP - 116-123
PB - ELSEVIER SCIENCE SA
SN - 02578972
KW - Plasma polymerization
KW - Cyclopropylamine
KW - Stable amine-rich films
KW - Quartz crystal microbalance
KW - Immunosensor
KW - Label-free detection
UR - http://www.sciencedirect.com/science/article/pii/S0257897215302747
L2 - http://www.sciencedirect.com/science/article/pii/S0257897215302747
N2 - Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, immobilization of the biorecognition biomolecules onto the sensor surface is always required. Cyclopropylamine pulsed plastha polymerization in radio frequency (RF) capacitive discharges was employed to deposit amine thin films on the gold electrode of quartz crystal microbalance (QCM) biosensors and the monoclonal antibody AL-01, specific to human serum albumin (HSA), was immobilized onto the plasma polymer surface. Two different amine plasma polymers were studied. They had a similar amount of primary amine groups, 13-1.5 at.%, as determined by chemical derivatization but their stability in water was different. The 1st type, deposited at the floating potential and 120 Pa, exhibited 16% thickness loss after 24 h in water whereas the 2nd type deposited at RF electrode and 50 Pa was completely stable (2% thickness loss). Glutaraldehyde (GA) coupling of AL-01 was employed for both types of the amine films and the performance of QCM immunosensors was evaluated by the immunoassay flow test A high-stability of the frequency signal was obtained in both the cases but the 2nd type of the film provided low response to HSA. It was explained by its highly cross-linked structure and steric hindrance of active sites. Three different antibody immobilization methods were explored for the 1st type of the film. Stable baseline, selective and high response were recorded for GA and sulfo-SMCC methods. The results confirmed that the presented methodologies for the grafting of biomolecules on the gold are highly efficient and very promising for future use in biosensing. (C) 2015 Elsevier B.V. All rights reserved.
ER -
MAKHNEVA, Ekaterina, Anton MANAKHOV, Petr SKLÁDAL a Lenka ZAJÍČKOVÁ. Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions. \textit{SURFACE \&{} COATINGS TECHNOLOGY}. LAUSANNE: ELSEVIER SCIENCE SA, 2016, roč.~290, March, s.~116-123. ISSN~0257-8972. Dostupné z: https://dx.doi.org/10.1016/j.surfcoat.2015.09.035.
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