Non-Enzymatic Oligomerization of 3 ', 5 ' Cyclic AMP

J 2016

Non-Enzymatic Oligomerization of 3 ', 5 ' Cyclic AMP

COSTANZO, Giovanna, Samanta PINO, Anna Maria TIMPERIO, Judit ŠPONEROVÁ, Jiří ŠPONER et. al.

Základní údaje

Originální název

Non-Enzymatic Oligomerization of 3 ', 5 ' Cyclic AMP

Autoři

COSTANZO, Giovanna (380 Itálie), Samanta PINO (380 Itálie), Anna Maria TIMPERIO (380 Itálie), Judit ŠPONEROVÁ (348 Maďarsko, garant, domácí), Jiří ŠPONER (203 Česká republika, domácí), Olga NOVAKOVA (203 Česká republika), Ondrej ŠEDO (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí) a Ernesto DI MAURO (380 Itálie)

Vydání

Plos one, San Francisco, Public Library of Science, 2016, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.806

Kód RIV

RIV/00216224:14740/16:00088766

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1371/journal.pone.0165723

UT WoS

000386714200045

Klíčová slova anglicky

TEMPLATE-DIRECTED SYNTHESIS; NUCLEOSIDE PHOSPHORYLATION; MONTMORILLONITE CATALYSIS; MOLECULAR-STRUCTURE; RIBOZYME ACTIVITY; RNA; OLIGONUCLEOTIDES; POLYMERIZATION; GMP; DNA

Štítky

CF PROT, rivok

Změněno: 10. 3. 2017 10:03, Mgr. Eva Špillingová

Anotace

V originále

Recent studies illustrate that short oligonucleotide sequences can be easily produced from nucleotide precursors in a template-free non-enzymatic way under dehydrating conditions, i.e. using essentially dry materials. Here we report that 3',5' cyclic AMP may also serve as a substrate of the reaction, which proceeds under moderate conditions yet with a lower efficiency than the previously reported oligomerization of 3',5' cyclic GMP. Optimally the oligomerization requires (i) a temperature of 80 degrees C, (ii) a neutral to alkaline environment and (iii) a time on the order of weeks. Differences in the yield and required reaction conditions of the oligomerizations utilizing 3', 5' cGMP and cAMP are discussed in terms of the crystal structures of the compounds. Polymerization of 3',5' cyclic nucleotides, whose paramount relevance in a prebiotic chemistry context has been widely accepted for decades, supports the possibility that the origin of extant genetic materials might have followed a direct uninterrupted path since its very beginning, starting from non-elaborately pre-activated monomer compounds and simple reactions.

Návaznosti

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

COSTANZO, Giovanna, Samanta PINO, Anna Maria TIMPERIO, Judit ŠPONEROVÁ, Jiří ŠPONER, Olga NOVAKOVA, Ondrej ŠEDO, Zbyněk ZDRÁHAL a Ernesto DI MAURO. Non-Enzymatic Oligomerization of 3 ', 5 ' Cyclic AMP. Plos one. San Francisco: Public Library of Science, 2016, roč. 11, č. 11, s. nestránkováno, 14 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0165723.

@article{1375143,
   author = {Costanzo, Giovanna and Pino, Samanta and Timperio, Anna Maria and Šponerová, Judit and Šponer, Jiří and Novakova, Olga and Šedo, Ondrej and Zdráhal, Zbyněk and Di Mauro, Ernesto},
   article_location = {San Francisco},
   article_number = {11},
   doi = {http://dx.doi.org/10.1371/journal.pone.0165723},
   keywords = {TEMPLATE-DIRECTED SYNTHESIS; NUCLEOSIDE PHOSPHORYLATION; MONTMORILLONITE CATALYSIS; MOLECULAR-STRUCTURE; RIBOZYME ACTIVITY; RNA; OLIGONUCLEOTIDES; POLYMERIZATION; GMP; DNA},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Non-Enzymatic Oligomerization of 3 ', 5 ' Cyclic AMP},
   url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165723},
   volume = {11},
   year = {2016}
}

TY  - JOUR
ID  - 1375143
AU  - Costanzo, Giovanna - Pino, Samanta - Timperio, Anna Maria - Šponerová, Judit - Šponer, Jiří - Novakova, Olga - Šedo, Ondrej - Zdráhal, Zbyněk - Di Mauro, Ernesto
PY  - 2016
TI  - Non-Enzymatic Oligomerization of 3 ', 5 ' Cyclic AMP
JF  - Plos one
VL  - 11
IS  - 11
SP  - nestránkováno
EP  - nestránkováno
PB  - Public Library of Science
SN  - 19326203
KW  - TEMPLATE-DIRECTED SYNTHESIS
KW  - NUCLEOSIDE PHOSPHORYLATION
KW  - MONTMORILLONITE CATALYSIS
KW  - MOLECULAR-STRUCTURE
KW  - RIBOZYME ACTIVITY
KW  - RNA
KW  - OLIGONUCLEOTIDES
KW  - POLYMERIZATION
KW  - GMP
KW  - DNA
UR  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165723
L2  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165723
N2  - Recent studies illustrate that short oligonucleotide sequences can be easily produced from nucleotide precursors in a template-free non-enzymatic way under dehydrating conditions, i.e. using essentially dry materials. Here we report that 3',5' cyclic AMP may also serve as a substrate of the reaction, which proceeds under moderate conditions yet with a lower efficiency than the previously reported oligomerization of 3',5' cyclic GMP. Optimally the oligomerization requires (i) a temperature of 80 degrees C, (ii) a neutral to alkaline environment and (iii) a time on the order of weeks. Differences in the yield and required reaction conditions of the oligomerizations utilizing 3', 5' cGMP and cAMP are discussed in terms of the crystal structures of the compounds. Polymerization of 3',5' cyclic nucleotides, whose paramount relevance in a prebiotic chemistry context has been widely accepted for decades, supports the possibility that the origin of extant genetic materials might have followed a direct uninterrupted path since its very beginning, starting from non-elaborately pre-activated monomer compounds and simple reactions.
ER  -

COSTANZO, Giovanna, Samanta PINO, Anna Maria TIMPERIO, Judit ŠPONEROVÁ, Jiří ŠPONER, Olga NOVAKOVA, Ondrej ŠEDO, Zbyněk ZDRÁHAL a Ernesto DI MAURO. Non-Enzymatic Oligomerization of 3 ', 5 ' Cyclic AMP. \textit{Plos one}. San Francisco: Public Library of Science, 2016, roč.~11, č.~11, s.~nestránkováno, 14 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0165723.

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