| OMELYANENKO, Anna, Petra SEKYROVÁ a Michael ANDÄNG. ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent manner. SpringerPlus. Cham: Springer International Publishing, 2016, roč. 5, January, s. nestránkováno, 10 s. ISSN 2193-1801. Dostupné z: https://dx.doi.org/10.1186/s40064-016-1678-7. |
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@article{1375514,
author = {Omelyanenko, Anna and Sekyrová, Petra and Andäng, Michael},
article_location = {Cham},
article_number = {January},
doi = {http://dx.doi.org/10.1186/s40064-016-1678-7},
keywords = {Embryonic stem cells; Ion channel modulator; ZD7288; Differentiation; Proliferation; Pluripotency; Cell cycle; Serum},
language = {eng},
issn = {2193-1801},
journal = {SpringerPlus},
title = {ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent manner},
url = {http://springerplus.springeropen.com/articles/10.1186/s40064-016-1678-7},
volume = {5},
year = {2016}
}
TY - JOUR
ID - 1375514
AU - Omelyanenko, Anna - Sekyrová, Petra - Andäng, Michael
PY - 2016
TI - ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent manner
JF - SpringerPlus
VL - 5
IS - January
SP - nestránkováno
EP - nestránkováno
PB - Springer International Publishing
SN - 21931801
KW - Embryonic stem cells
KW - Ion channel modulator
KW - ZD7288
KW - Differentiation
KW - Proliferation
KW - Pluripotency
KW - Cell cycle
KW - Serum
UR - http://springerplus.springeropen.com/articles/10.1186/s40064-016-1678-7
L2 - http://springerplus.springeropen.com/articles/10.1186/s40064-016-1678-7
N2 - Pluripotent stem cells are the starting cell type of choice for the development of many cell-based regenerative therapies due to their rapid and unlimited proliferation and broad differentiation potential. The unique pluripotent cell cycle underlies both these properties. Hyperpolarization-activated cyclic nucleotide-gated cation (HCN) family channels have previously been reported to modulate mouse embryonic stem cell (ESC) proliferation and here we characterize the effects of HCN inhibitor ZD7288 on ESC proliferation and stem cell identity. The doubling time of cells treated with the HCN blocker increased by similar to 30 % due to longer G1 and S phases, resulting in a nearly twofold reduction in ESC numbers after 4 day serum-free culture. Slower progression through S phase was not accompanied by H2AX phosphorylation or cell stalling at transition points, although EdU incorporation in treated cells was reduced. Despite the drastic cell cycle perturbations, the pluripotent status of the cells was not compromised by treatment. Cultures treated with the HCN blocker in maintenance conditions maintained pluripotency marker expression on both RNA and protein level, although we observed a reversible effect on morphology and colony formation frequency. Addition of ZD7288 in differentiating media improved FBS-driven differentiation, but not directed differentiation to neuroectoderm, further indicating that altered cell cycle structure does not necessarily compromise pluripotency and drive ESCs to differentiation. The categorically different outcomes of ZD7288 use during differentiation indicate that cell culture context can be determinative for effects of ion-modulatory molecules and underscores the need for exploring their action in serum-free conditions demanded by potential clinical use.
ER -
OMELYANENKO, Anna, Petra SEKYROVÁ a Michael ANDÄNG. ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent manner. \textit{SpringerPlus}. Cham: Springer International Publishing, 2016, roč.~5, January, s.~nestránkováno, 10 s. ISSN~2193-1801. Dostupné z: https://dx.doi.org/10.1186/s40064-016-1678-7.
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