Capillary electrophoresis in the analysis of biologically
important thiols

J 2017

Capillary electrophoresis in the analysis of biologically important thiols

LAČNÁ, Júlia, František FORET a Petr KUBÁŇ

Základní údaje

Originální název

Capillary electrophoresis in the analysis of biologically important thiols

Název česky

Využití kapilární elektroforézy v analýze biologicky aktivních thiolů.

Autoři

LAČNÁ, Júlia (703 Slovensko, domácí), František FORET (203 Česká republika, domácí) a Petr KUBÁŇ (203 Česká republika, garant, domácí)

Vydání

Electrophoresis, HOBOKEN, WILEY-BLACKWELL, 2017, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.569

Kód RIV

RIV/00216224:14740/17:00094683

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/elps.201600354

UT WoS

000392422100013

Klíčová slova česky

Biologické thioly, kapilární elektroforéza, klinikcé aplikace, review

Klíčová slova anglicky

Biological thiols; Capillary electrophoresis; Clinical applications; Review

Štítky

OA, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 6. 2017 15:27, Mgr. Eva Špillingová

Anotace

V originále

In this review article, CE methods for analysis of biologically important thiols are overviewed. The article covers the period from the previously published comprehensive review in 2004 until mid-2016, with emphasis on various detection modes, novel approaches for sample preconcentration, and applications in clinical practice. The most commonly used detection methods, such as conductometry or absorbance detection, although universally applicable and available in most commercial instruments have low sensitivity and have only limited use in thiol analysis. Amperometric and MS detection are more sensitive and have their steady place in thiol analysis, although the mainstay remains CE with LIF detection, reaching nanomolar concentration sensitivities for most of the thiols. Novel probes for CE-LIF have been developed and tested. The preconcentration approaches using modified gold nanoparticles reaching excellent sensitivities in the picomolar range and various sample stacking methods are also reviewed. Finally, significant clinical applications of the developed methods are discussed with critical insights into the future of CE analysis of thiols.

Návaznosti

GA13-21919S, projekt VaV

Název: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Grantová agentura ČR, Vývoj neinvazivních metod odberu vzorku a analýzy v point-of-care dignostice biologických materiálu

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

LAČNÁ, Júlia, František FORET a Petr KUBÁŇ. Capillary electrophoresis in the analysis of biologically important thiols. Electrophoresis. HOBOKEN: WILEY-BLACKWELL, 2017, roč. 38, č. 1, s. 203-222. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201600354.

@article{1376188,
   author = {Lačná, Júlia and Foret, František and Kubáň, Petr},
   article_location = {HOBOKEN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1002/elps.201600354},
   keywords = {Biological thiols; Capillary electrophoresis; Clinical applications; Review},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Capillary electrophoresis in the analysis of biologically important thiols},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/elps.201600354/abstract},
   volume = {38},
   year = {2017}
}

TY  - JOUR
ID  - 1376188
AU  - Lačná, Júlia - Foret, František - Kubáň, Petr
PY  - 2017
TI  - Capillary electrophoresis in the analysis of biologically important thiols
JF  - Electrophoresis
VL  - 38
IS  - 1
SP  - 203-222
EP  - 203-222
PB  - WILEY-BLACKWELL
SN  - 01730835
KW  - Biological thiols
KW  - Capillary electrophoresis
KW  - Clinical applications
KW  - Review
UR  - http://onlinelibrary.wiley.com/doi/10.1002/elps.201600354/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/elps.201600354/abstract
N2  - In this review article, CE methods for analysis of biologically important thiols are overviewed. The article covers the period from the previously published comprehensive review in 2004 until mid-2016, with emphasis on various detection modes, novel approaches for sample preconcentration, and applications in clinical practice. The most commonly used detection methods, such as conductometry or absorbance detection, although universally applicable and available in most commercial instruments have low sensitivity and have only limited use in thiol analysis. Amperometric and MS detection are more sensitive and have their steady place in thiol analysis, although the mainstay remains CE with LIF detection, reaching nanomolar concentration sensitivities for most of the thiols. Novel probes for CE-LIF have been developed and tested. The preconcentration approaches using modified gold nanoparticles reaching excellent sensitivities in the picomolar range and various sample stacking methods are also reviewed. Finally, significant clinical applications of the developed methods are discussed with critical insights into the future of CE analysis of thiols.
ER  -

LAČNÁ, Júlia, František FORET a Petr KUBÁŇ. Capillary electrophoresis in the analysis of biologically important thiols. \textit{Electrophoresis}. HOBOKEN: WILEY-BLACKWELL, 2017, roč.~38, č.~1, s.~203-222. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201600354.

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