Capillary electrophoresis in the analysis of biologically
important thiols

J 2017

Capillary electrophoresis in the analysis of biologically important thiols

LAČNÁ, Júlia, František FORET and Petr KUBÁŇ

Basic information

Original name

Capillary electrophoresis in the analysis of biologically important thiols

Name in Czech

Využití kapilární elektroforézy v analýze biologicky aktivních thiolů.

Authors

LAČNÁ, Júlia (703 Slovakia, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Electrophoresis, HOBOKEN, WILEY-BLACKWELL, 2017, 0173-0835

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.569

RIV identification code

RIV/00216224:14740/17:00094683

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/elps.201600354

UT WoS

000392422100013

Keywords (in Czech)

Biologické thioly, kapilární elektroforéza, klinikcé aplikace, review

Keywords in English

Biological thiols; Capillary electrophoresis; Clinical applications; Review

Abstract

V originále

In this review article, CE methods for analysis of biologically important thiols are overviewed. The article covers the period from the previously published comprehensive review in 2004 until mid-2016, with emphasis on various detection modes, novel approaches for sample preconcentration, and applications in clinical practice. The most commonly used detection methods, such as conductometry or absorbance detection, although universally applicable and available in most commercial instruments have low sensitivity and have only limited use in thiol analysis. Amperometric and MS detection are more sensitive and have their steady place in thiol analysis, although the mainstay remains CE with LIF detection, reaching nanomolar concentration sensitivities for most of the thiols. Novel probes for CE-LIF have been developed and tested. The preconcentration approaches using modified gold nanoparticles reaching excellent sensitivities in the picomolar range and various sample stacking methods are also reviewed. Finally, significant clinical applications of the developed methods are discussed with critical insights into the future of CE analysis of thiols.

Links

GA13-21919S, research and development project

Name: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

LAČNÁ, Júlia, František FORET and Petr KUBÁŇ. Capillary electrophoresis in the analysis of biologically important thiols. Electrophoresis. HOBOKEN: WILEY-BLACKWELL, 2017, vol. 38, No 1, p. 203-222. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201600354.

@article{1376188,
   author = {Lačná, Júlia and Foret, František and Kubáň, Petr},
   article_location = {HOBOKEN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1002/elps.201600354},
   keywords = {Biological thiols; Capillary electrophoresis; Clinical applications; Review},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Capillary electrophoresis in the analysis of biologically important thiols},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/elps.201600354/abstract},
   volume = {38},
   year = {2017}
}

TY  - JOUR
ID  - 1376188
AU  - Lačná, Júlia - Foret, František - Kubáň, Petr
PY  - 2017
TI  - Capillary electrophoresis in the analysis of biologically important thiols
JF  - Electrophoresis
VL  - 38
IS  - 1
SP  - 203-222
EP  - 203-222
PB  - WILEY-BLACKWELL
SN  - 01730835
KW  - Biological thiols
KW  - Capillary electrophoresis
KW  - Clinical applications
KW  - Review
UR  - http://onlinelibrary.wiley.com/doi/10.1002/elps.201600354/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/elps.201600354/abstract
N2  - In this review article, CE methods for analysis of biologically important thiols are overviewed. The article covers the period from the previously published comprehensive review in 2004 until mid-2016, with emphasis on various detection modes, novel approaches for sample preconcentration, and applications in clinical practice. The most commonly used detection methods, such as conductometry or absorbance detection, although universally applicable and available in most commercial instruments have low sensitivity and have only limited use in thiol analysis. Amperometric and MS detection are more sensitive and have their steady place in thiol analysis, although the mainstay remains CE with LIF detection, reaching nanomolar concentration sensitivities for most of the thiols. Novel probes for CE-LIF have been developed and tested. The preconcentration approaches using modified gold nanoparticles reaching excellent sensitivities in the picomolar range and various sample stacking methods are also reviewed. Finally, significant clinical applications of the developed methods are discussed with critical insights into the future of CE analysis of thiols.
ER  -

LAČNÁ, Júlia, František FORET and Petr KUBÁŇ. Capillary electrophoresis in the analysis of biologically important thiols. \textit{Electrophoresis}. HOBOKEN: WILEY-BLACKWELL, 2017, vol.~38, No~1, p.~203-222. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.201600354.

Detailed Information on Publication Record