Cell Proliferation Analysis Using EdU Labeling in Whole Plant
and Histological Samples of Arabidopsis

C 2016

Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis

KAZDA, Anita, Svetlana AKIMCHEVA, Matthew J. WATSON a Karel ŘÍHA

Základní údaje

Originální název

Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis

Autoři

KAZDA, Anita (40 Rakousko), Svetlana AKIMCHEVA (40 Rakousko), Matthew J. WATSON (40 Rakousko) a Karel ŘÍHA (203 Česká republika, garant, domácí)

Vydání

New York, Plant Cell Division - Methods and Protocols, od s. 169-182, 14 s. Methods in Molecular Biology, 1370, 2016

Nakladatel

Springer

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14740/16:00093963

Organizační jednotka

Středoevropský technologický institut

ISBN

978-1-4939-3141-5

DOI

http://dx.doi.org/10.1007/978-1-4939-3142-2_13

UT WoS

000369085800014

Klíčová slova anglicky

5-Ethynyl-2´deoxyuridine; Cell division; Cell proliferation; DNA labeling; DNA replication; EdU; Histology

Štítky

rivok

Změněno: 24. 4. 2020 12:50, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The ability to analyze cell division in both spatial and temporal dimensions within an organism is a key requirement in developmental biology. Specialized cell types within individual organs, such as those within shoot and root apical meristems, have often been identified by differences in their rates of proliferation prior to the characterization of distinguishing molecular markers. Replication-dependent labeling of DNA is a widely used method for assaying cell proliferation. The earliest approaches used radioactive labeling with tritiated thymidine, which were later followed by immunodetection of bromodeoxyuridine (BrdU). A major advance in DNA labeling came with the use of 5-ethynyl-2´deoxyuridine (EdU) which has proven to have multiple advantages over BrdU. Here we describe the methodology for analyzing EdU labeling and retention in whole plants and histological sections of Arabidopsis.

Citovat

KAZDA, Anita, Svetlana AKIMCHEVA, Matthew J. WATSON a Karel ŘÍHA. Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis. In Marie-Cécile Caillaud. Plant Cell Division - Methods and Protocols. New York: Springer, 2016, s. 169-182. Methods in Molecular Biology, 1370. ISBN 978-1-4939-3141-5. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-3142-2_13.

@inbook{1376226,
   author = {Kazda, Anita and Akimcheva, Svetlana and Watson, Matthew J. and Říha, Karel},
   address = {New York},
   booktitle = {Plant Cell Division - Methods and Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-4939-3142-2_13},
   editor = {Marie-Cécile Caillaud},
   keywords = {5-Ethynyl-2´deoxyuridine; Cell division; Cell proliferation; DNA labeling; DNA replication; EdU; Histology},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-4939-3141-5},
   pages = {169-182},
   publisher = {Springer},
   title = {Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis},
   url = {http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13},
   year = {2016}
}

TY  - CHAP
ID  - 1376226
AU  - Kazda, Anita - Akimcheva, Svetlana - Watson, Matthew J. - Říha, Karel
PY  - 2016
TI  - Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis
VL  - Methods in Molecular Biology, 1370
PB  - Springer
CY  - New York
SN  - 9781493931415
KW  - 5-Ethynyl-2´deoxyuridine
KW  - Cell division
KW  - Cell proliferation
KW  - DNA labeling
KW  - DNA replication
KW  - EdU
KW  - Histology
UR  - http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13
L2  - http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13
N2  - The ability to analyze cell division in both spatial and temporal dimensions within an organism is a key requirement in developmental biology. Specialized cell types within individual organs, such as those within shoot and root apical meristems, have often been identified by differences in their rates of proliferation prior to the characterization of distinguishing molecular markers. Replication-dependent labeling of DNA is a widely used method for assaying cell proliferation. The earliest approaches used radioactive labeling with tritiated thymidine, which were later followed by immunodetection of bromodeoxyuridine (BrdU). A major advance in DNA labeling came with the use of 5-ethynyl-2´deoxyuridine (EdU) which has proven to have multiple advantages over BrdU. Here we describe the methodology for analyzing EdU labeling and retention in whole plants and histological sections of Arabidopsis.
ER  -

KAZDA, Anita, Svetlana AKIMCHEVA, Matthew J. WATSON a Karel ŘÍHA. Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis. In Marie-Cécile Caillaud. \textit{Plant Cell Division - Methods and Protocols}. New York: Springer, 2016, s.~169-182. Methods in Molecular Biology, 1370. ISBN~978-1-4939-3141-5. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-3142-2\_{}13.

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