Characterization of total retinoid-like activity of compounds
produced by three common phytoplankton species

J 2016

Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species

SYCHROVÁ, Eliška, Jana PRIEBOJOVÁ, Marie SMUTNÁ, Kateřina NOVÁKOVÁ, Jiří KOHOUTEK et. al.

Základní údaje

Originální název

Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species

Autoři

SYCHROVÁ, Eliška (203 Česká republika, domácí), Jana PRIEBOJOVÁ (703 Slovensko, domácí), Marie SMUTNÁ (203 Česká republika, domácí), Kateřina NOVÁKOVÁ (203 Česká republika, domácí), Jiří KOHOUTEK (203 Česká republika, domácí) a Klára HILSCHEROVÁ (203 Česká republika, garant, domácí)

Vydání

Harmful Algae, AMSTERDAM (NETHERLANDS), Elsevier Science, 2016, 1568-9883

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10000 1. Natural Sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.087

Kód RIV

RIV/00216224:14310/16:00088800

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.hal.2016.11.002

UT WoS

000390516200015

Klíčová slova anglicky

Retinoic acid receptor; Cyanobacteria; Biomass extracts; Exudates; All-trans-retinoic acid; 9-Cis retinoic acid; Retinoid-like activity; Microcystin; Light conditions

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 4. 2017 18:54, Ing. Andrea Mikešková

Anotace

V originále

Phytoplankton can produce various bioactive metabolites, which may affect other organisms in the aquatic environment. This study provides the first information on the total retinoid-like activity associated with both intracellular and extracellular metabolites produced by selected phytoplankton species that could play a role in teratogenic effects and developmental disruption in exposed organisms. The studied species included a coccoid cyanobacteria, a filamentous cyanobacteria and a green alga, all of which commonly occur in freshwater bodies in Europe. Methanolic extracts from cellular material and extracellular exudates were prepared from cultures cultivated in two light-intensity variants with five replicates for each species. The retinoid-like activity was evaluated by in vitro assays along with chemical analyses of two potent retinoic acids (all-trans retinoic acid (ATRA) and 9cis-RA). The mean total retinoid-like activity of metabolites produced by the three studied species representing different phytoplankton taxonomic groups ranged from 705 to 5572 ng ATRA equivalent/g dry matter corresponding to 0.064-0.234 ng ATRA/10(6) cells. Retinoid-like activity was found in the cellular extracts of all species, while only the extracellular exudates of cyanobacteria exhibited detectable activity (41-1081 ng ATRA/L). The greatest extracellular as well as total (extra- and intra- cellular together) retinoid-like activity was detected for Microcystis aeruginosa. The two potent retinoic acids studied were more frequently detected in cellular extracts than in extracellular exudates of all species. The results indicate possible influence of light intensity and cell density on the production of metabolites with retinoid-like activity and the cyanotoxin microcystin by the studied species.

Návaznosti

GP14-29370P, projekt VaV

Název: Běžné druhy sinic jako producenti teratogenních retinoidních látek

Investor: Grantová agentura ČR, Běžné druhy sinic jako producenti teratogenních retinoidních látek

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

SYCHROVÁ, Eliška, Jana PRIEBOJOVÁ, Marie SMUTNÁ, Kateřina NOVÁKOVÁ, Jiří KOHOUTEK a Klára HILSCHEROVÁ. Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species. Harmful Algae. AMSTERDAM (NETHERLANDS): Elsevier Science, 2016, roč. 60, December, s. 157-166. ISSN 1568-9883. Dostupné z: https://dx.doi.org/10.1016/j.hal.2016.11.002.

@article{1376647,
   author = {Sychrová, Eliška and Priebojová, Jana and Smutná, Marie and Nováková, Kateřina and Kohoutek, Jiří and Hilscherová, Klára},
   article_location = {AMSTERDAM (NETHERLANDS)},
   article_number = {December},
   doi = {http://dx.doi.org/10.1016/j.hal.2016.11.002},
   keywords = {Retinoic acid receptor; Cyanobacteria; Biomass extracts; Exudates; All-trans-retinoic acid; 9-Cis retinoic acid; Retinoid-like activity; Microcystin; Light conditions},
   language = {eng},
   issn = {1568-9883},
   journal = {Harmful Algae},
   title = {Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species},
   url = {http://www.sciencedirect.com/science/article/pii/S156898831630021X},
   volume = {60},
   year = {2016}
}

TY  - JOUR
ID  - 1376647
AU  - Sychrová, Eliška - Priebojová, Jana - Smutná, Marie - Nováková, Kateřina - Kohoutek, Jiří - Hilscherová, Klára
PY  - 2016
TI  - Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species
JF  - Harmful Algae
VL  - 60
IS  - December
SP  - 157-166
EP  - 157-166
PB  - Elsevier Science
SN  - 15689883
KW  - Retinoic acid receptor
KW  - Cyanobacteria
KW  - Biomass extracts
KW  - Exudates
KW  - All-trans-retinoic acid
KW  - 9-Cis retinoic acid
KW  - Retinoid-like activity
KW  - Microcystin
KW  - Light conditions
UR  - http://www.sciencedirect.com/science/article/pii/S156898831630021X
L2  - http://www.sciencedirect.com/science/article/pii/S156898831630021X
N2  - Phytoplankton can produce various bioactive metabolites, which may affect other organisms in the aquatic environment. This study provides the first information on the total retinoid-like activity associated with both intracellular and extracellular metabolites produced by selected phytoplankton species that could play a role in teratogenic effects and developmental disruption in exposed organisms. The studied species included a coccoid cyanobacteria, a filamentous cyanobacteria and a green alga, all of which commonly occur in freshwater bodies in Europe. Methanolic extracts from cellular material and extracellular exudates were prepared from cultures cultivated in two light-intensity variants with five replicates for each species. The retinoid-like activity was evaluated by in vitro assays along with chemical analyses of two potent retinoic acids (all-trans retinoic acid (ATRA) and 9cis-RA). The mean total retinoid-like activity of metabolites produced by the three studied species representing different phytoplankton taxonomic groups ranged from 705 to 5572 ng ATRA equivalent/g dry matter corresponding to 0.064-0.234 ng ATRA/10(6) cells. Retinoid-like activity was found in the cellular extracts of all species, while only the extracellular exudates of cyanobacteria exhibited detectable activity (41-1081 ng ATRA/L). The greatest extracellular as well as total (extra- and intra- cellular together) retinoid-like activity was detected for Microcystis aeruginosa. The two potent retinoic acids studied were more frequently detected in cellular extracts than in extracellular exudates of all species. The results indicate possible influence of light intensity and cell density on the production of metabolites with retinoid-like activity and the cyanotoxin microcystin by the studied species.
ER  -

SYCHROVÁ, Eliška, Jana PRIEBOJOVÁ, Marie SMUTNÁ, Kateřina NOVÁKOVÁ, Jiří KOHOUTEK a Klára HILSCHEROVÁ. Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species. \textit{Harmful Algae}. AMSTERDAM (NETHERLANDS): Elsevier Science, 2016, roč.~60, December, s.~157-166. ISSN~1568-9883. Dostupné z: https://dx.doi.org/10.1016/j.hal.2016.11.002.

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