Influence of Trichobilharzia regenti (Digenea:
Schistosomatidae) on the Defence Activity of Radix lagotis
(Lymnaeidae) Haemocytes

J 2014

Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the Defence Activity of Radix lagotis (Lymnaeidae) Haemocytes

SKÁLA, Vladimír, Alena ČERNÍKOVÁ, Zuzana JINDROVÁ, Martin KAŠNÝ, Martin VOSTRÝ et. al.

Základní údaje

Originální název

Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the Defence Activity of Radix lagotis (Lymnaeidae) Haemocytes

Autoři

SKÁLA, Vladimír (203 Česká republika), Alena ČERNÍKOVÁ (203 Česká republika), Zuzana JINDROVÁ (203 Česká republika), Martin KAŠNÝ (203 Česká republika, garant, domácí), Martin VOSTRÝ (203 Česká republika), Anthony J. WALKER (826 Velká Británie a Severní Irsko) a Petr HORÁK (203 Česká republika)

Vydání

PLOS ONE, Public Library of Science, 2014, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.234

Kód RIV

RIV/00216224:14310/14:00094007

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0111696

UT WoS

000344556900065

Klíčová slova anglicky

RESISTANT BIOMPHALARIA-GLABRATA; ECHINOSTOMA-PARAENSEI; STAGNALIS HEMOCYTES; PROTEIN-KINASE; ELECTRON-MICROSCOPY; AVIAN SCHISTOSOME; IMMUNE-RESPONSES; SNAIL HOSTS; IN-VITRO; MANSONI

Štítky

AKR, rivok

Změněno: 8. 3. 2018 12:29, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

Radix lagotis is an intermediate snail host of the nasal bird schistosome Trichobilharzia regenti. Changes in defence responses in infected snails that might be related to host-parasite compatibility are not known. This study therefore aimed to characterize R. lagotis haemocyte defence mechanisms and determine the extent to which they are modulated by T. regenti. Histological observations of R. lagotis infected with T. regenti revealed that early phases of infection were accompanied by haemocyte accumulation around the developing larvae 2-36 h post exposure (p. e.) to the parasite. At later time points, 44-92 h p. e., no haemocytes were observed around T. regenti. Additionally, microtubular aggregates likely corresponding to phagocytosed ciliary plates of T. regenti miracidia were observed within haemocytes by use of transmission electron microscopy. When the infection was in the patent phase, haemocyte phagocytic activity and hydrogen peroxide production were significantly reduced in infected R. lagotis when compared to uninfected counterparts, whereas haemocyte abundance increased in infected snails. At a molecular level, protein kinase C (PKC) and extracellular-signal regulated kinase (ERK) were found to play an important role in regulating these defence reactions in R. lagotis. Moreover, haemocytes from snails with patent infection displayed lower PKC and ERK activity in cell adhesion assays when compared to those from uninfected snails, which may therefore be related to the reduced defence activities of these cells. These data provide the first integrated insight into the immunobiology of R. lagotis and demonstrate modulation of haemocyte-mediated responses in patent T. regenti infected snails. Given that immunomodulation occurs during patency, interference of snail-host defence by T. regenti might be important for the sustained production and/or release of infective cercariae.

Citovat

SKÁLA, Vladimír, Alena ČERNÍKOVÁ, Zuzana JINDROVÁ, Martin KAŠNÝ, Martin VOSTRÝ, Anthony J. WALKER a Petr HORÁK. Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the Defence Activity of Radix lagotis (Lymnaeidae) Haemocytes. PLOS ONE. Public Library of Science, 2014, roč. 9, č. 11, s. "nestrankovano", 11 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0111696.

@article{1376687,
   author = {Skála, Vladimír and Černíková, Alena and Jindrová, Zuzana and Kašný, Martin and Vostrý, Martin and Walker, Anthony J. and Horák, Petr},
   article_number = {11},
   doi = {http://dx.doi.org/10.1371/journal.pone.0111696},
   keywords = {RESISTANT BIOMPHALARIA-GLABRATA; ECHINOSTOMA-PARAENSEI; STAGNALIS HEMOCYTES; PROTEIN-KINASE; ELECTRON-MICROSCOPY; AVIAN SCHISTOSOME; IMMUNE-RESPONSES; SNAIL HOSTS; IN-VITRO; MANSONI},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the Defence Activity of Radix lagotis (Lymnaeidae) Haemocytes},
   volume = {9},
   year = {2014}
}

TY  - JOUR
ID  - 1376687
AU  - Skála, Vladimír - Černíková, Alena - Jindrová, Zuzana - Kašný, Martin - Vostrý, Martin - Walker, Anthony J. - Horák, Petr
PY  - 2014
TI  - Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the Defence Activity of Radix lagotis (Lymnaeidae) Haemocytes
JF  - PLOS ONE
VL  - 9
IS  - 11
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - Public Library of Science
SN  - 19326203
KW  - RESISTANT BIOMPHALARIA-GLABRATA
KW  - ECHINOSTOMA-PARAENSEI
KW  - STAGNALIS HEMOCYTES
KW  - PROTEIN-KINASE
KW  - ELECTRON-MICROSCOPY
KW  - AVIAN SCHISTOSOME
KW  - IMMUNE-RESPONSES
KW  - SNAIL HOSTS
KW  - IN-VITRO
KW  - MANSONI
N2  - Radix lagotis is an intermediate snail host of the nasal bird schistosome Trichobilharzia regenti. Changes in defence responses in infected snails that might be related to host-parasite compatibility are not known. This study therefore aimed to characterize R. lagotis haemocyte defence mechanisms and determine the extent to which they are modulated by T. regenti. Histological observations of R. lagotis infected with T. regenti revealed that early phases of infection were accompanied by haemocyte accumulation around the developing larvae 2-36 h post exposure (p. e.) to the parasite. At later time points, 44-92 h p. e., no haemocytes were observed around T. regenti. Additionally, microtubular aggregates likely corresponding to phagocytosed ciliary plates of T. regenti miracidia were observed within haemocytes by use of transmission electron microscopy. When the infection was in the patent phase, haemocyte phagocytic activity and hydrogen peroxide production were significantly reduced in infected R. lagotis when compared to uninfected counterparts, whereas haemocyte abundance increased in infected snails. At a molecular level, protein kinase C (PKC) and extracellular-signal regulated kinase (ERK) were found to play an important role in regulating these defence reactions in R. lagotis. Moreover, haemocytes from snails with patent infection displayed lower PKC and ERK activity in cell adhesion assays when compared to those from uninfected snails, which may therefore be related to the reduced defence activities of these cells. These data provide the first integrated insight into the immunobiology of R. lagotis and demonstrate modulation of haemocyte-mediated responses in patent T. regenti infected snails. Given that immunomodulation occurs during patency, interference of snail-host defence by T. regenti might be important for the sustained production and/or release of infective cercariae.
ER  -

SKÁLA, Vladimír, Alena ČERNÍKOVÁ, Zuzana JINDROVÁ, Martin KAŠNÝ, Martin VOSTRÝ, Anthony J. WALKER a Petr HORÁK. Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the Defence Activity of Radix lagotis (Lymnaeidae) Haemocytes. \textit{PLOS ONE}. Public Library of Science, 2014, roč.~9, č.~11, s.~''nestrankovano'', 11 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0111696.

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