Novel Temperate Phages of Salmonella enterica subsp salamae and
subsp diarizonae and Their Activity against Pathogenic
S-enterica subsp enterica Isolates

J 2017

Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates

MIKALOVÁ, Lenka, Juraj BOSÁK, Hana HŘÍBKOVÁ, Daniela DĚDIČOVÁ, Oldřich BENADA et. al.

Základní údaje

Originální název

Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates

Autoři

MIKALOVÁ, Lenka (203 Česká republika, domácí), Juraj BOSÁK (703 Slovensko, domácí), Hana HŘÍBKOVÁ (203 Česká republika, domácí), Daniela DĚDIČOVÁ (203 Česká republika), Oldřich BENADA (203 Česká republika), Jan ŠMARDA (203 Česká republika, domácí) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.766

Kód RIV

RIV/00216224:14110/17:00094691

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0170734

UT WoS

000396160500007

Klíčová slova anglicky

TAILED-BACTERIOPHAGES; COMPARATIVE GENOMICS; ENORMOUS DIVERSITY; ESCHERICHIA-COLI; EVOLUTION; TAXONOMY; PROPHAGES; BACTERIAL; ORIGINS; SERVER

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2018 13:39, Soňa Böhmová

Anotace

V originále

Forty strains of Salmonella enterica (S. enterica) subspecies salamae (II), arizonae (IIIa), diarizonae (IIIb), and houtenae (IV) were isolated from human or environmental samples and tested for bacteriophage production. Production of bacteriophages was observed in 15 S. enterica strains (37.5%) belonging to either the subspecies salamae (8 strains) or diarizonae (7 strains). Activity of phages was tested against 52 pathogenic S. enterica subsp. enterica isolates and showed that phages produced by subsp. salamae had broader activity against pathogenic salmonellae compared to phages from the subsp. diarizonae. All 15 phages were analyzed using PCR amplification of phage-specific regions and 9 different amplification profiles were identified. Five phages (SEN1, SEN4, SEN5, SEN22, and SEN34) were completely sequenced and classified as temperate phages. Phages SEN4 and SEN5 were genetically identical, thus representing a single phage type (i.e. SEN4/5). SEN1 and SEN4/5 fit into the group of P2-like phages, while the SEN22 phage showed sequence relatedness to P22-like phages. Interestingly, while phage SEN34 was genetically distantly related to Lambda-like phages (Siphoviridae), it had the morphology of the Myoviridae family. Based on sequence analysis and electron microscopy, phages SEN1 and SEN4/5 were members of the Myoviridae family and phage SEN22 belonged to the Podoviridae family.

Návaznosti

GA16-21649S, projekt VaV

Název: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Grantová agentura ČR, Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

MUNI/11/InGA04/2014, interní kód MU

Název: Alternativní antimikrobiální látky: studium vazby kolicinů na povrch patogenní bakterie

Investor: Masarykova univerzita, Alternativní antimikrobiální látky: studium vazby kolicinů na povrch patogenní bakterie

ROZV/20/LF/2015, interní kód MU

Název: LF - Příspěvek IP 2015

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek IP 2015

Citovat

MIKALOVÁ, Lenka, Juraj BOSÁK, Hana HŘÍBKOVÁ, Daniela DĚDIČOVÁ, Oldřich BENADA, Jan ŠMARDA a David ŠMAJS. Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates. Plos one. San Francisco: Public Library of Science, 2017, roč. 12, č. 1, s. 1-14. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0170734.

@article{1376729,
   author = {Mikalová, Lenka and Bosák, Juraj and Hříbková, Hana and Dědičová, Daniela and Benada, Oldřich and Šmarda, Jan and Šmajs, David},
   article_location = {San Francisco},
   article_number = {1},
   doi = {http://dx.doi.org/10.1371/journal.pone.0170734},
   keywords = {TAILED-BACTERIOPHAGES; COMPARATIVE GENOMICS; ENORMOUS DIVERSITY; ESCHERICHIA-COLI; EVOLUTION; TAXONOMY; PROPHAGES; BACTERIAL; ORIGINS; SERVER},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1376729
AU  - Mikalová, Lenka - Bosák, Juraj - Hříbková, Hana - Dědičová, Daniela - Benada, Oldřich - Šmarda, Jan - Šmajs, David
PY  - 2017
TI  - Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates
JF  - Plos one
VL  - 12
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - Public Library of Science
SN  - 19326203
KW  - TAILED-BACTERIOPHAGES
KW  - COMPARATIVE GENOMICS
KW  - ENORMOUS DIVERSITY
KW  - ESCHERICHIA-COLI
KW  - EVOLUTION
KW  - TAXONOMY
KW  - PROPHAGES
KW  - BACTERIAL
KW  - ORIGINS
KW  - SERVER
N2  - Forty strains of Salmonella enterica (S. enterica) subspecies salamae (II), arizonae (IIIa), diarizonae (IIIb), and houtenae (IV) were isolated from human or environmental samples and tested for bacteriophage production. Production of bacteriophages was observed in 15 S. enterica strains (37.5%) belonging to either the subspecies salamae (8 strains) or diarizonae (7 strains). Activity of phages was tested against 52 pathogenic S. enterica subsp. enterica isolates and showed that phages produced by subsp. salamae had broader activity against pathogenic salmonellae compared to phages from the subsp. diarizonae. All 15 phages were analyzed using PCR amplification of phage-specific regions and 9 different amplification profiles were identified. Five phages (SEN1, SEN4, SEN5, SEN22, and SEN34) were completely sequenced and classified as temperate phages. Phages SEN4 and SEN5 were genetically identical, thus representing a single phage type (i.e. SEN4/5). SEN1 and SEN4/5 fit into the group of P2-like phages, while the SEN22 phage showed sequence relatedness to P22-like phages. Interestingly, while phage SEN34 was genetically distantly related to Lambda-like phages (Siphoviridae), it had the morphology of the Myoviridae family. Based on sequence analysis and electron microscopy, phages SEN1 and SEN4/5 were members of the Myoviridae family and phage SEN22 belonged to the Podoviridae family.
ER  -

MIKALOVÁ, Lenka, Juraj BOSÁK, Hana HŘÍBKOVÁ, Daniela DĚDIČOVÁ, Oldřich BENADA, Jan ŠMARDA a David ŠMAJS. Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates. \textit{Plos one}. San Francisco: Public Library of Science, 2017, roč.~12, č.~1, s.~1-14. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0170734.

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