J 2013

The Structure and Host Entry of an Invertebrate Parvovirus

MENG, Geng, Xinzheng ZHANG, Pavel PLEVKA, Qian YU, Peter TIJSSEN et. al.

Základní údaje

Originální název

The Structure and Host Entry of an Invertebrate Parvovirus

Autoři

MENG, Geng, Xinzheng ZHANG, Pavel PLEVKA, Qian YU, Peter TIJSSEN a Michael G. ROSSMANN

Vydání

JOURNAL OF VIROLOGY, WASHINGTON, AMER SOC MICROBIOLOGY, 2013, 0022-538X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.648

Organizační jednotka

Středoevropský technologický institut

UT WoS

000327183800002

Štítky

Změněno: 29. 3. 2017 11:08, Mgr. Eva Špillingová

Anotace

V originále

The 3.5-angstrom resolution X-ray crystal structure of mature cricket parvovirus (Acheta domesticus densovirus [AdDNV]) has been determined. Structural comparisons show that vertebrate and invertebrate parvoviruses have evolved independently, although there are common structural features among all parvovirus capsid proteins. It was shown that raising the temperature of the AdDNV particles caused a loss of their genomes. The structure of these emptied particles was determined by cryo-electron microscopy to 5.5-angstrom resolution, and the capsid structure was found to be the same as that for the full, mature virus except for the absence of the three ordered nucleotides observed in the crystal structure. The viral protein 1 (VP1) amino termini could be externalized without significant damage to the capsid. In vitro, this externalization of the VP1 amino termini is accompanied by the release of the viral genome.