| ZHANG, Xinzheng, Ju SHENG, Pavel PLEVKA, Richard J. KUHN, Michael S. DIAMOND and Michael G. ROSSMANN. Dengue structure differs at the temperatures of its human and mosquito hosts. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: National Academy of Sciences, 2013, vol. 110, No 17, p. 6795-6799. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1304300110. |
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@article{1376801,
author = {Zhang, Xinzheng and Sheng, Ju and Plevka, Pavel and Kuhn, Richard J. and Diamond, Michael S. and Rossmann, Michael G.},
article_location = {WASHINGTON},
article_number = {17},
doi = {http://dx.doi.org/10.1073/pnas.1304300110},
keywords = {host temperature; irreversible and conformational change; temperature dependence; cryo-EM},
language = {eng},
issn = {0027-8424},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
title = {Dengue structure differs at the temperatures of its human and mosquito hosts},
volume = {110},
year = {2013}
}
TY - JOUR
ID - 1376801
AU - Zhang, Xinzheng - Sheng, Ju - Plevka, Pavel - Kuhn, Richard J. - Diamond, Michael S. - Rossmann, Michael G.
PY - 2013
TI - Dengue structure differs at the temperatures of its human and mosquito hosts
JF - Proceedings of the National Academy of Sciences of the United States of America
VL - 110
IS - 17
SP - 6795-6799
EP - 6795-6799
PB - National Academy of Sciences
SN - 00278424
KW - host temperature
KW - irreversible and conformational change
KW - temperature dependence
KW - cryo-EM
N2 - We report on a conformational transition of dengue virus when changing the temperature from that present in its mosquito vectors to that of its human host. Using cryoelectron microscopy, we show that although the virus has a smooth surface, a diameter of similar to 500 angstrom, and little exposed membrane at room temperature, the virions have a bumpy appearance with a diameter of similar to 550 angstrom and some exposed membrane at 37 degrees C. The bumpy structure at 37 degrees C was found to be similar to the previously predicted structure of an intermediate between the smooth mature and fusogenic forms. As humans have a body temperature of 37 degrees C, the bumpy form of the virus would be the form present in humans. Thus, optimal dengue virus vaccines should induce antibodies that preferentially recognize epitopes exposed on the bumpy form of the virus.
ER -
ZHANG, Xinzheng, Ju SHENG, Pavel PLEVKA, Richard J. KUHN, Michael S. DIAMOND and Michael G. ROSSMANN. Dengue structure differs at the temperatures of its human and mosquito hosts. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: National Academy of Sciences, 2013, vol.~110, No~17, p.~6795-6799. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1304300110.
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