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@article{1376840,
author = {Jasnovidova, Olga and Krejčíková, Magdaléna and Kubíček, Karel and Štefl, Richard},
article_location = {Hoboken},
article_number = {6},
doi = {http://dx.doi.org/10.15252/embr.201643723},
keywords = {NMR; RNA processing; RNAPII CTD code; structural biology},
language = {eng},
issn = {1469-221X},
journal = {EMBO reports},
title = {Structural insight into recognition of phosphorylated threonine-4 of RNA polymerase II C-terminal domain by Rtt103p},
url = {http://embor.embopress.org/content/18/6/906},
volume = {18},
year = {2017}
}
TY - JOUR
ID - 1376840
AU - Jasnovidova, Olga - Krejčíková, Magdaléna - Kubíček, Karel - Štefl, Richard
PY - 2017
TI - Structural insight into recognition of phosphorylated threonine-4 of RNA polymerase II C-terminal domain by Rtt103p
JF - EMBO reports
VL - 18
IS - 6
SP - 906-913
EP - 906-913
PB - Wiley-Blackwell
SN - 1469221X
KW - NMR
KW - RNA processing
KW - RNAPII CTD code
KW - structural biology
UR - http://embor.embopress.org/content/18/6/906
L2 - http://embor.embopress.org/content/18/6/906
N2 - Phosphorylation patterns of the C-terminal domain (CTD) of largest subunit of RNA polymerase II (called the CTD code) orchestrate the recruitment of RNA processing and transcription factors. Recent studies showed that not only serines and tyrosines but also threonines of the CTD can be phosphorylated with a number of functional consequences, including the interaction with yeast transcription termination factor, Rtt103p. Here, we report the solution structure of the Rtt103p CTD-interacting domain (CID) bound to Thr4 phosphorylated CTD, a poorly understood letter of the CTD code. The structure reveals a direct recognition of the phospho-Thr4 mark by Rtt103p CID and extensive interactions involving residues from three repeats of the CTD heptad. Intriguingly, Rtt103p's CID binds equally well Thr4 and Ser2 phosphorylated CTD. A doubly phosphorylated CTD at Ser2 and Thr4 diminishes its binding affinity due to electrostatic repulsion. Our structural data suggest that the recruitment of a CID-containing CTD-binding factor may be coded by more than one letter of the CTD code.
ER -
JASNOVIDOVA, Olga, Magdaléna KREJČÍKOVÁ, Karel KUBÍČEK and Richard ŠTEFL. Structural insight into recognition of phosphorylated threonine-4 of RNA polymerase II C-terminal domain by Rtt103p. \textit{EMBO reports}. Hoboken: Wiley-Blackwell, 2017, vol.~18, No~6, p.~906-913. ISSN~1469-221X. Available from: https://dx.doi.org/10.15252/embr.201643723.
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