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@article{1377005, author = {Kushkevych, Ivan}, article_location = {Warsaw}, article_number = {2}, keywords = {kinetic analysis; inflammatory bowel diseases; pyruvate ferredoxin oxidoreductase; sulfate-reducing bacteria}, language = {eng}, issn = {1733-1331}, journal = {Polish Journal of Microbiology}, note = {Nelze přenášet do RIV - doc. Kushkevych nemá na WoS afiliaci na MU, nýbrž na NAAS Ukraine, Inst Anim Biol, Lvov, Ukraine}, title = {Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9}, url = {http://www.pjm.microbiology.pl/archive/vol6422015107.pdf}, volume = {64}, year = {2015} }
TY - JOUR ID - 1377005 AU - Kushkevych, Ivan PY - 2015 TI - Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 JF - Polish Journal of Microbiology VL - 64 IS - 2 SP - 107-114 EP - 107-114 PB - POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS SN - 17331331 N1 - Nelze přenášet do RIV - doc. Kushkevych nemá na WoS afiliaci na MU, nýbrž na NAAS Ukraine, Inst Anim Biol, Lvov, Ukraine KW - kinetic analysis KW - inflammatory bowel diseases KW - pyruvate ferredoxin oxidoreductase KW - sulfate-reducing bacteria UR - http://www.pjm.microbiology.pl/archive/vol6422015107.pdf N2 - Intestinal sulfate-reducing bacteria reduce sulfate ions to hydrogen sulfide causing inflammatory bowel diseases of humans and animals. The bacteria consume lactate as electron donor which is oxidized to acetate via pyruvate in process of the dissimilatory sulfate reduction. Pyruvate-ferredoxin oxidoreductase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria Desulfovibrio piger and Desulfomicrobium sp. have never been well-characterized and have not been yet studied. In this paper we present for the first time the specific activity of pyruvate-ferredoxin oxidoreductase and the kinetic properties of the enzyme in cell-free extracts of both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains. Microbiological, biochemical, biophysical and statistical methods were used in this work. The optimal temperature (+35°C) and pH 8.5 for enzyme reaction were determined. The spectral analysis of the purified pyruvate-ferredoxin oxidoreductase from the cell-free extracts was demonstrated. Analysis of the kinetic properties of the studied enzyme was carried out. Initial (instantaneous) reaction velocity (V0), maximum amount of the product of reaction (Pmax), the reaction time (half saturation period) and maximum velocity of the pyruvate-ferredoxin oxidoreductase reaction (Vmax) were defined. Michaelis constants (Km) of the enzyme reaction were calculated for both intestinal bacterial strains. The studies of the kinetic enzyme properties in the intestinal sulfate-reducing bacteria strains in detail can be prospects for clarifying the etiological role of these bacteria in the development of inflammatory bowel diseases ER -
KUSHKEVYCH, Ivan. Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9. \textit{Polish Journal of Microbiology}. Warsaw: POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2015, roč.~64, č.~2, s.~107-114. ISSN~1733-1331.
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