J 2015

Activity and kinetic properties of phosphotransacetylase from intestinal sulfate-reducing bacteria

KUSHKEVYCH, Ivan

Základní údaje

Originální název

Activity and kinetic properties of phosphotransacetylase from intestinal sulfate-reducing bacteria

Autoři

Vydání

ACTA BIOCHIMICA POLONICA, 2015, 0001-527X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 1.187

Organizační jednotka

Přírodovědecká fakulta

DOI

UT WoS

000351891600014

Klíčová slova anglicky

sulfate-reducing bacteria; phosphotransacetylase; kinetic analysis; inflammatory bowel diseases

Štítky

Změněno: 6. 3. 2020 09:00, Mgr. Marie Šípková, DiS.

Anotace

V originále

Phosphotransacetylase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria Desulfovibrio piger and Desulfomicrobium sp. has never been well-characterized and has not been studied yet. In this paper, the specific activity of phosphotransacetylase and the kinetic properties of the enzyme in cell-free extracts of both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains were presented at the first time. The microbiological, biochemical, biophysical and statistical methods in this work were used. The optimal temperature and pH for enzyme reaction was determined. Analysis of the kinetic properties of the studied enzyme was carried out. Initial (instantaneous) reaction velocity (V-0), maximum amount of the product of reaction (P-max), the reaction time (half saturation period, tau) and maximum velocity of the phosphotransacetylase reaction (V-max) were defined. Michaelis constants (K-m) of the enzyme reaction (3.36 +/- 0.35 mM for D. piger Vib-7, 5.97 +/- 0.62 mM for Desulfomicrobium sp. Rod-9) were calculated. The studies of the phosphotransacetylase in the process of dissimilatory sulfate reduction and kinetic properties of this enzyme in intestinal sulfate-reducing bacteria, their production of acetate in detail can be perspective for clarification of their etiological role in the development of the humans and animals bowel diseases. These studies might help in predicting the development of diseases of the gastrointestinal tract, by providing further details on the etiology of bowel diseases which are very important for the clinical diagnosis of these disease types.