Amino Acid Profiling of Zinc Resistant Prostate Cancer Cell
Lines: Associations With Cancer Progression

J 2017

Amino Acid Profiling of Zinc Resistant Prostate Cancer Cell Lines: Associations With Cancer Progression

KRATOCHVÍLOVÁ, Monika, Martina RAUDENSKÁ, Zbynek HEGER, Lukas RICHTERA, Natalia CERNEI et. al.

Základní údaje

Originální název

Amino Acid Profiling of Zinc Resistant Prostate Cancer Cell Lines: Associations With Cancer Progression

Autoři

KRATOCHVÍLOVÁ, Monika (203 Česká republika, domácí), Martina RAUDENSKÁ (203 Česká republika, domácí), Zbynek HEGER (203 Česká republika), Lukas RICHTERA (203 Česká republika), Natalia CERNEI (203 Česká republika), Vojtech ADAM (203 Česká republika), Petr BABULA (203 Česká republika, domácí), Marie NOVÁKOVÁ (203 Česká republika, domácí), Michal MASAŘÍK (203 Česká republika, garant, domácí) a Jaromír GUMULEC (203 Česká republika, domácí)

Vydání

Prostate, Hoboken, Wiley-Blackwell, 2017, 0270-4137

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.347

Kód RIV

RIV/00216224:14110/17:00096422

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/pros.23304

UT WoS

000397496300005

Klíčová slova anglicky

amino acid; aspartate; metabolomics; resistance; zinc

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2018 17:02, Soňa Böhmová

Anotace

V originále

BACKGROUND: Failure in intracellular zinc accumulation is a key process in prostate carcinogenesis. Nevertheless, epidemiological studies of zinc administration have provided contradicting results. In order to examine the impact of the artificial intracellular increase of zinc(II) ions on prostate cancer metabolism, PNT1A, 22Rv1, and PC-3 prostatic cell lines—depicting different stages of cancer progression—and their zinc-resistant counterparts were used. To determine “benign” and “malignant” metabolic profiles, amino acid patterns, gene expression, and antioxidant capacity of these cell lines were assessed. METHODS: Amino acid profiles were examined using an ion-exchange liquid chromatography. Intracellular zinc content was measured by atomic absorption spectrometry. Metallothionein was quantified using differential pulse voltammetry. The content of reduced glutathione was determined using high performance liquid chromatography coupled with an electrochemical detector. Cellular antioxidant capacity was determined by the ABTS test and gene expression analysis was performed by qRT-PCR. RESULTS AND CONCLUSIONS: Long-term zinc treatment was shown to reroute cell metabolism from benign to more malignant type. Long-term application of high concentration of zinc(II) significantly enhanced cisplatin resistance, invasiveness, cellular antioxidant capacity, synthesis of glutathione, and expression of treatment resistance- and stemness-associated genes (SOX2, POU5F1, BIRC5). Tumorous cell lines universally displayed high accumulation of aspartate and sarcosine and depletion of essential amino acids. Increased aspartate/threonine, aspartate/methionine, and sarcosine/serine ratios were associated with cancer phenotype with high levels of sensitivity and specificity.

Návaznosti

MUNI/A/1365/2015, interní kód MU

Název: Kardiovaskulární systém: od modelu přes terapii k prevenci (Akronym: KAMOTEPRE)

Investor: Masarykova univerzita, Kardiovaskulární systém: od modelu přes terapii k prevenci, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

ROZV/20/LF/2015, interní kód MU

Název: LF - Příspěvek IP 2015

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek IP 2015

Citovat

KRATOCHVÍLOVÁ, Monika, Martina RAUDENSKÁ, Zbynek HEGER, Lukas RICHTERA, Natalia CERNEI, Vojtech ADAM, Petr BABULA, Marie NOVÁKOVÁ, Michal MASAŘÍK a Jaromír GUMULEC. Amino Acid Profiling of Zinc Resistant Prostate Cancer Cell Lines: Associations With Cancer Progression. Prostate. Hoboken: Wiley-Blackwell, 2017, roč. 77, č. 6, s. 604-616. ISSN 0270-4137. Dostupné z: https://dx.doi.org/10.1002/pros.23304.

@article{1377538,
   author = {Kratochvílová, Monika and Raudenská, Martina and Heger, Zbynek and Richtera, Lukas and Cernei, Natalia and Adam, Vojtech and Babula, Petr and Nováková, Marie and Masařík, Michal and Gumulec, Jaromír},
   article_location = {Hoboken},
   article_number = {6},
   doi = {http://dx.doi.org/10.1002/pros.23304},
   keywords = {amino acid; aspartate; metabolomics; resistance; zinc},
   language = {eng},
   issn = {0270-4137},
   journal = {Prostate},
   title = {Amino Acid Profiling of Zinc Resistant Prostate Cancer Cell Lines: Associations With Cancer Progression},
   volume = {77},
   year = {2017}
}

TY  - JOUR
ID  - 1377538
AU  - Kratochvílová, Monika - Raudenská, Martina - Heger, Zbynek - Richtera, Lukas - Cernei, Natalia - Adam, Vojtech - Babula, Petr - Nováková, Marie - Masařík, Michal - Gumulec, Jaromír
PY  - 2017
TI  - Amino Acid Profiling of Zinc Resistant Prostate Cancer Cell Lines: Associations With Cancer Progression
JF  - Prostate
VL  - 77
IS  - 6
SP  - 604-616
EP  - 604-616
PB  - Wiley-Blackwell
SN  - 02704137
KW  - amino acid
KW  - aspartate
KW  - metabolomics
KW  - resistance
KW  - zinc
N2  - BACKGROUND: Failure in intracellular zinc accumulation is a key process in prostate carcinogenesis. Nevertheless, epidemiological studies of zinc administration have provided contradicting results. In order to examine the impact of the artificial intracellular increase of zinc(II) ions on prostate cancer metabolism, PNT1A, 22Rv1, and PC-3 prostatic cell lines—depicting different stages of cancer progression—and their zinc-resistant counterparts were used. To determine “benign” and “malignant” metabolic profiles, amino acid patterns, gene expression, and antioxidant capacity of these cell lines were assessed. METHODS: Amino acid profiles were examined using an ion-exchange liquid chromatography. Intracellular zinc content was measured by atomic absorption spectrometry. Metallothionein was quantified using differential pulse voltammetry. The content of reduced glutathione was determined using high performance liquid chromatography coupled with an electrochemical detector. Cellular antioxidant capacity was determined by the ABTS test and gene expression analysis was performed by qRT-PCR. RESULTS AND CONCLUSIONS: Long-term zinc treatment was shown to reroute cell metabolism from benign to more malignant type. Long-term application of high concentration of zinc(II) significantly enhanced cisplatin resistance, invasiveness, cellular antioxidant capacity, synthesis of glutathione, and expression of treatment resistance- and stemness-associated genes (SOX2, POU5F1, BIRC5). Tumorous cell lines universally displayed high accumulation of aspartate and sarcosine and depletion of essential amino acids. Increased aspartate/threonine, aspartate/methionine, and sarcosine/serine ratios were associated with cancer phenotype with high levels of sensitivity and specificity.
ER  -

KRATOCHVÍLOVÁ, Monika, Martina RAUDENSKÁ, Zbynek HEGER, Lukas RICHTERA, Natalia CERNEI, Vojtech ADAM, Petr BABULA, Marie NOVÁKOVÁ, Michal MASAŘÍK a Jaromír GUMULEC. Amino Acid Profiling of Zinc Resistant Prostate Cancer Cell Lines: Associations With Cancer Progression. \textit{Prostate}. Hoboken: Wiley-Blackwell, 2017, roč.~77, č.~6, s.~604-616. ISSN~0270-4137. Dostupné z: https://dx.doi.org/10.1002/pros.23304.

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