| SVITKOVA, Veronika, Jana BLASKOVICOVA, Monika TEKELOVA, Brigitta Margit KALLAI, Teodora IGNAT, Veronika HORÁČKOVÁ, Petr SKLÁDAL, Pavel KOPEL, Vojtěch ADAM, Dana FARKASOVA a Ján LABUDA. Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution. Sensors and Actuators B: Chemical. Lausanne: Elsevier, 2017, roč. 243, May, s. 435-444. ISSN 0925-4005. Dostupné z: https://dx.doi.org/10.1016/j.snb.2016.11.160. |
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@article{1380698,
author = {Svitkova, Veronika and Blaskovicova, Jana and Tekelova, Monika and Kallai, Brigitta Margit and Ignat, Teodora and Horáčková, Veronika and Skládal, Petr and Kopel, Pavel and Adam, Vojtěch and Farkasova, Dana and Labuda, Ján},
article_location = {Lausanne},
article_number = {May},
doi = {http://dx.doi.org/10.1016/j.snb.2016.11.160},
keywords = {Nanoparticles toxicity; Quantum dots; UV-C radiation; DNA damage; Biosensor; Biosensing},
language = {eng},
issn = {0925-4005},
journal = {Sensors and Actuators B: Chemical},
title = {Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution},
url = {http://www.sciencedirect.com/science/article/pii/S0925400516319566},
volume = {243},
year = {2017}
}
TY - JOUR
ID - 1380698
AU - Svitkova, Veronika - Blaskovicova, Jana - Tekelova, Monika - Kallai, Brigitta Margit - Ignat, Teodora - Horáčková, Veronika - Skládal, Petr - Kopel, Pavel - Adam, Vojtěch - Farkasova, Dana - Labuda, Ján
PY - 2017
TI - Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution
JF - Sensors and Actuators B: Chemical
VL - 243
IS - May
SP - 435-444
EP - 435-444
PB - Elsevier
SN - 09254005
KW - Nanoparticles toxicity
KW - Quantum dots
KW - UV-C radiation
KW - DNA damage
KW - Biosensor
KW - Biosensing
UR - http://www.sciencedirect.com/science/article/pii/S0925400516319566
L2 - http://www.sciencedirect.com/science/article/pii/S0925400516319566
N2 - Oxidation stress induced by nanoparticles is considered to be the main mechanism of their toxicity. An evaluation of oxidative damage to DNA, one of the mostly endangered molecules, belongs to general approaches used in nanomaterials cytotoxicology. However, for spherical, biochemical and other reasons, they should be differences in nanoparticles behaviour using tests based on cells and pure nucleic acid substances. The goal of the work is to detect structural damage to isolated salmon sperm double stranded DNA at its exposure to UV-C radiation (254 nm) in the presence of thiol-capped CdS quantum dots of various size and fluorescence intensity. An electrochemical DNA-based biosensor composed of a glassy carbon electrode with a layered structure of chemical modifiers dsDNA and CdS (Ps was prepared and used for the voltammetric and impedimetric evaluation of a DNA portion survived after the irradiation. The cyclic voltammetric response of the redox indicator system hexacyanoferrate(III/II) and square wave voltammetric response of the guanine and adenine moieties were measured as analytical signals. The results were verified by experiments with irradiation of DNA in the solution phase followed by voltammetric measurement of immobilized survived portion of DNA (biosensing) and by conventional agarose gel electrophoresis. Experiments showed significant effect of the fluorescent active quantum dots on the degree of DNA degradation. (C) 2016 Published by Elsevier B.V.
ER -
SVITKOVA, Veronika, Jana BLASKOVICOVA, Monika TEKELOVA, Brigitta Margit KALLAI, Teodora IGNAT, Veronika HORÁČKOVÁ, Petr SKLÁDAL, Pavel KOPEL, Vojtěch ADAM, Dana FARKASOVA a Ján LABUDA. Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution. \textit{Sensors and Actuators B: Chemical}. Lausanne: Elsevier, 2017, roč.~243, May, s.~435-444. ISSN~0925-4005. Dostupné z: https://dx.doi.org/10.1016/j.snb.2016.11.160.
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