Epitope mapping of Borrelia burgdorferi OspC protein in
homodimeric fold

J 2017

Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold

NOREK, Adam a Lubomír JANDA

Základní údaje

Originální název

Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold

Autoři

NOREK, Adam (203 Česká republika, garant, domácí) a Lubomír JANDA (203 Česká republika, domácí)

Vydání

Protein Science, HOBOKEN, WILEY, 2017, 0961-8368

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.410

Kód RIV

RIV/00216224:14740/17:00096691

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/pro.3125

UT WoS

000398183800013

Klíčová slova anglicky

epitope mapping; monoclonal antibody; outer surface protein C; OspC; Borrelia burgdorferi; protein alignment; immunoprecipitation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 2. 2018 10:39, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

In current work, we used recombinant OspC protein derived from B. afzelii strain BRZ31 in the native homodimeric fold for mice immunization and following selection process to produce three mouse monoclonal antibodies able to bind to variable parts of up to five different OspC proteins. Applying the combination of mass spectrometry assisted epitope mapping and affinity based theoretical prediction we have localized regions responsible for antigen-antibody interactions and approximate epitopes' amino acid composition. Two mAbs (3F4 and 2A9) binds to linear epitopes located in previously described immunogenic regions in the exposed part of OspC protein. The third mAb (2D1) recognises highly conserved discontinuous epitope close to the ligand binding domain 1.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

NOREK, Adam a Lubomír JANDA. Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold. Protein Science. HOBOKEN: WILEY, 2017, roč. 26, č. 4, s. 796-806. ISSN 0961-8368. Dostupné z: https://dx.doi.org/10.1002/pro.3125.

@article{1380716,
   author = {Norek, Adam and Janda, Lubomír},
   article_location = {HOBOKEN},
   article_number = {4},
   doi = {http://dx.doi.org/10.1002/pro.3125},
   keywords = {epitope mapping; monoclonal antibody; outer surface protein C; OspC; Borrelia burgdorferi; protein alignment; immunoprecipitation},
   language = {eng},
   issn = {0961-8368},
   journal = {Protein Science},
   title = {Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/pro.3125/abstract},
   volume = {26},
   year = {2017}
}

TY  - JOUR
ID  - 1380716
AU  - Norek, Adam - Janda, Lubomír
PY  - 2017
TI  - Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold
JF  - Protein Science
VL  - 26
IS  - 4
SP  - 796-806
EP  - 796-806
PB  - WILEY
SN  - 09618368
KW  - epitope mapping
KW  - monoclonal antibody
KW  - outer surface protein C
KW  - OspC
KW  - Borrelia burgdorferi
KW  - protein alignment
KW  - immunoprecipitation
UR  - http://onlinelibrary.wiley.com/doi/10.1002/pro.3125/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/pro.3125/abstract
N2  - In current work, we used recombinant OspC protein derived from B. afzelii strain BRZ31 in the native homodimeric fold for mice immunization and following selection process to produce three mouse monoclonal antibodies able to bind to variable parts of up to five different OspC proteins. Applying the combination of mass spectrometry assisted epitope mapping and affinity based theoretical prediction we have localized regions responsible for antigen-antibody interactions and approximate epitopes' amino acid composition. Two mAbs (3F4 and 2A9) binds to linear epitopes located in previously described immunogenic regions in the exposed part of OspC protein. The third mAb (2D1) recognises highly conserved discontinuous epitope close to the ligand binding domain 1.
ER  -

NOREK, Adam a Lubomír JANDA. Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold. \textit{Protein Science}. HOBOKEN: WILEY, 2017, roč.~26, č.~4, s.~796-806. ISSN~0961-8368. Dostupné z: https://dx.doi.org/10.1002/pro.3125.

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