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@article{1380716,
author = {Norek, Adam and Janda, Lubomír},
article_location = {HOBOKEN},
article_number = {4},
doi = {http://dx.doi.org/10.1002/pro.3125},
keywords = {epitope mapping; monoclonal antibody; outer surface protein C; OspC; Borrelia burgdorferi; protein alignment; immunoprecipitation},
language = {eng},
issn = {0961-8368},
journal = {Protein Science},
title = {Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold},
url = {http://onlinelibrary.wiley.com/doi/10.1002/pro.3125/abstract},
volume = {26},
year = {2017}
}
TY - JOUR
ID - 1380716
AU - Norek, Adam - Janda, Lubomír
PY - 2017
TI - Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold
JF - Protein Science
VL - 26
IS - 4
SP - 796-806
EP - 796-806
PB - WILEY
SN - 09618368
KW - epitope mapping
KW - monoclonal antibody
KW - outer surface protein C
KW - OspC
KW - Borrelia burgdorferi
KW - protein alignment
KW - immunoprecipitation
UR - http://onlinelibrary.wiley.com/doi/10.1002/pro.3125/abstract
L2 - http://onlinelibrary.wiley.com/doi/10.1002/pro.3125/abstract
N2 - In current work, we used recombinant OspC protein derived from B. afzelii strain BRZ31 in the native homodimeric fold for mice immunization and following selection process to produce three mouse monoclonal antibodies able to bind to variable parts of up to five different OspC proteins. Applying the combination of mass spectrometry assisted epitope mapping and affinity based theoretical prediction we have localized regions responsible for antigen-antibody interactions and approximate epitopes' amino acid composition. Two mAbs (3F4 and 2A9) binds to linear epitopes located in previously described immunogenic regions in the exposed part of OspC protein. The third mAb (2D1) recognises highly conserved discontinuous epitope close to the ligand binding domain 1.
ER -
NOREK, Adam a Lubomír JANDA. Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold. \textit{Protein Science}. HOBOKEN: WILEY, 2017, roč.~26, č.~4, s.~796-806. ISSN~0961-8368. Dostupné z: https://dx.doi.org/10.1002/pro.3125.
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