2017
Maleic anhydride and acetylene plasma co-polymers for the development of effective QCM and SPR immunosensors
MAKHNEVA, Ekaterina, Zdeněk FARKA, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Petr SKLÁDAL et. al.Základní údaje
Originální název
Maleic anhydride and acetylene plasma co-polymers for the development of effective QCM and SPR immunosensors
Autoři
MAKHNEVA, Ekaterina, Zdeněk FARKA, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Petr SKLÁDAL a Lenka ZAJÍČKOVÁ
Vydání
CEITEC PhD Retreat II, 2017
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10305 Fluids and plasma physics
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Organizační jednotka
Středoevropský technologický institut
ISBN
978-80-210-8550-3
Příznaky
Mezinárodní význam
Změněno: 16. 5. 2017 14:49, doc. Mgr. Zdeněk Farka, Ph.D.
Anotace
V originále
Biosensors have been extensively developed and applied for biomedical and environmental studies. Quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) biosensors appeared to be very promising tools regarding the sensitive determination of various antigens and bacteria. For the development of effective biosensors, the immobilization of the biorecognition biomolecules onto the sensor surface is always required. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity allowing the formation of the covalent linkages between biomolecules and a surface. For most applications, a sufficiently high surface concentration of functional groups as well as the layer stability in different buffers is required. In our work, stable carboxyl-rich plasma polymers (COOH PPs) were deposited using plasma co-polymerization of maleic anhydride and acetylene at atmospheric pressure using dielectric barrier discharge (DBD). Concentrations of carboxyl groups were determined by well-known derivatization with trifluoroethanol (TFE). For the immunosensors development 20 nm thick COOH PPs were deposited onto the gold surfaces of QCM electrodes and SPR chips. Then the monoclonal antibody AL-01, specific to human serum albumin (HSA) was immobilized using EDC/NHS activation of COOH groups in the polymer. Performance of QCM and SPR immunosensors was evaluated by the immunoassay flow test.
Návaznosti
LQ1601, projekt VaV |
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