Biofilm formation, antibiotic susceptibility and RAPD genotypes
in Pseudomonas aeruginosa clinical strains isolated from single
centre intensive care unit patients

J 2017

Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients

VANERKOVA, M., B. MALISOVA, I. KOTASKOVA, Veronika HOLÁ, Filip RŮŽIČKA et. al.

Základní údaje

Originální název

Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients

Autoři

VANERKOVA, M. (203 Česká republika), B. MALISOVA (203 Česká republika), I. KOTASKOVA (203 Česká republika), Veronika HOLÁ (203 Česká republika, domácí), Filip RŮŽIČKA (203 Česká republika, domácí) a Tomáš FREIBERGER (203 Česká republika, garant, domácí)

Vydání

Folia microbiologica, Praha, Mikrobiologický ústav Praha AV ČR, 2017, 0015-5632

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.311

Kód RIV

RIV/00216224:14110/17:00095637

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s12223-017-0526-7

UT WoS

000412456500011

Klíčová slova anglicky

Pseudomonas aeruginosa

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2018 13:31, Soňa Böhmová

Anotace

V originále

The aim of this study was to analyse genotypes, antimicrobial susceptibility patterns and serotypes in Pseudomonas aeruginosa clinical strains, including the clonal dissemination of particular strains throughout various intensive care units in one medical centre. Using random amplified polymorphic DNA (RAPD-PCR) and P. aeruginosa antisera, 22 different genotypes and 8 serotypes were defined among 103 isolates from 48 patients. No direct association between P. aeruginosa strain genotypes and serotypes was observed. RAPD typing in strains with the same serotype revealed different genotypes and, on the contrary, most strains with a different serotype displayed the same amplification pattern. The resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients. A higher degree of antibiotic resistance and stronger biofilm production in common genotypes compared to rare ones and genetic homogeneity of the most resistant strains indicated the role of antibiotic pressure in acquiring resistant and more virulent strains in our hospital. In conclusion, genetic characterisation of P. aeruginosa strains using RAPD method was shown to be more accurate in epidemiological analyses than phenotyping.

Návaznosti

NV16-31593A, projekt VaV

Název: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

VANERKOVA, M., B. MALISOVA, I. KOTASKOVA, Veronika HOLÁ, Filip RŮŽIČKA a Tomáš FREIBERGER. Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients. Folia microbiologica. Praha: Mikrobiologický ústav Praha AV ČR, 2017, roč. 62, č. 6, s. 531-538. ISSN 0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-017-0526-7.

@article{1380793,
   author = {Vanerkova, M. and Malisova, B. and Kotaskova, I. and Holá, Veronika and Růžička, Filip and Freiberger, Tomáš},
   article_location = {Praha},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s12223-017-0526-7},
   keywords = {Pseudomonas aeruginosa},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia microbiologica},
   title = {Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients},
   volume = {62},
   year = {2017}
}

TY  - JOUR
ID  - 1380793
AU  - Vanerkova, M. - Malisova, B. - Kotaskova, I. - Holá, Veronika - Růžička, Filip - Freiberger, Tomáš
PY  - 2017
TI  - Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients
JF  - Folia microbiologica
VL  - 62
IS  - 6
SP  - 531-538
EP  - 531-538
PB  - Mikrobiologický ústav Praha AV ČR
SN  - 00155632
KW  - Pseudomonas aeruginosa
N2  - The aim of this study was to analyse genotypes, antimicrobial susceptibility patterns and serotypes in Pseudomonas aeruginosa clinical strains, including the clonal dissemination of particular strains throughout various intensive care units in one medical centre. Using random amplified polymorphic DNA (RAPD-PCR) and P. aeruginosa antisera, 22 different genotypes and 8 serotypes were defined among 103 isolates from 48 patients. No direct association between P. aeruginosa strain genotypes and serotypes was observed. RAPD typing in strains with the same serotype revealed different genotypes and, on the contrary, most strains with a different serotype displayed the same amplification pattern. The resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients. A higher degree of antibiotic resistance and stronger biofilm production in common genotypes compared to rare ones and genetic homogeneity of the most resistant strains indicated the role of antibiotic pressure in acquiring resistant and more virulent strains in our hospital. In conclusion, genetic characterisation of P. aeruginosa strains using RAPD method was shown to be more accurate in epidemiological analyses than phenotyping.
ER  -

VANERKOVA, M., B. MALISOVA, I. KOTASKOVA, Veronika HOLÁ, Filip RŮŽIČKA a Tomáš FREIBERGER. Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients. \textit{Folia microbiologica}. Praha: Mikrobiologický ústav Praha AV ČR, 2017, roč.~62, č.~6, s.~531-538. ISSN~0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-017-0526-7.

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