Cyclopropylamine plasma polymer surfaces in quartz crystal
microbalance and surface plasmon resonance immunosensing

D 2017

Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing

MAKHNEVA, Ekaterina, Zdeněk FARKA, Petr SKLÁDAL a Lenka ZAJÍČKOVÁ

Základní údaje

Originální název

Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing

Autoři

MAKHNEVA, Ekaterina (643 Rusko, domácí), Zdeněk FARKA (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí) a Lenka ZAJÍČKOVÁ (203 Česká republika, garant, domácí)

Vydání

Brno, 8th International Conference on Nanomaterials - Research and Application, NANOCON 2016, od s. 395-401, 7 s. 2017

Nakladatel

TANGER Ltd.

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10305 Fluids and plasma physics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

paměťový nosič (CD, DVD, flash disk)

Kód RIV

RIV/00216224:14740/17:00096777

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-87294-71-0

UT WoS

000410656100069

Klíčová slova anglicky

Cyclopropylamine; Immunosensor; Label-free detection; Plasma polymerization; Quartz crystal microbalance; Stable amine-rich films; Surface plasmon resonance

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 3. 2018 20:20, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Immunosensors with highly sensitive and rapid detection capabilities of various biomolecules are of a great demand in the field of biomedicine and environmental control. The two mostly employed transducer principles are either optical surface plasmon resonance (SPR) and mass sensitive quartz crystal microbalance (QCM). The first task encountered in the immunosensor development is the preparation of a coating matrix suitable for the immobilization of antibody on the golden layer of SPR or QCM. The most popular approaches are based on wet chemical treatments such as self-assembled monolayers (SAMs) of alkanethiols, disulfides, or polyethylenimine (PEI). However, these approaches suffer from several drawbacks such as a poor stability, long time preparation, unstable baseline or a high level of noise. As an alternative to SAMs, the deposition of thin functional coatings by plasma polymerization can be employed. The plasma polymerization has already been successfully applied to the deposition of thin films containing carboxyl, amine, anhydride groups. In this work, pulsed plasma polymerization of cyclopropylamine is employed to deposit stable amine-rich thin films on the surfaces of QCM and SPR chips. The AL-01 antibody, specific to human serum albumin (HSA), was attached to the QCM and SPR chips surfaces via glutaraldehyde activation. For the developed immunosensors the stable baseline was recorded. Selective and high response was achieved during the reaction with the solution of antigen. The results confirmed that the introduced methodology have a great potential for biosensing applications.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MAKHNEVA, Ekaterina, Zdeněk FARKA, Petr SKLÁDAL a Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing. In 8th International Conference on Nanomaterials - Research and Application, NANOCON 2016. Brno: TANGER Ltd., 2017, s. 395-401. ISBN 978-80-87294-71-0.

@inproceedings{1381386,
   author = {Makhneva, Ekaterina and Farka, Zdeněk and Skládal, Petr and Zajíčková, Lenka},
   address = {Brno},
   booktitle = {8th International Conference on Nanomaterials - Research and Application, NANOCON 2016},
   keywords = {Cyclopropylamine; Immunosensor; Label-free detection; Plasma polymerization; Quartz crystal microbalance; Stable amine-rich films; Surface plasmon resonance},
   howpublished = {paměťový nosič},
   language = {eng},
   location = {Brno},
   isbn = {978-80-87294-71-0},
   pages = {395-401},
   publisher = {TANGER Ltd.},
   title = {Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing},
   year = {2017}
}

TY  - JOUR
ID  - 1381386
AU  - Makhneva, Ekaterina - Farka, Zdeněk - Skládal, Petr - Zajíčková, Lenka
PY  - 2017
TI  - Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing
PB  - TANGER Ltd.
CY  - Brno
SN  - 9788087294710
KW  - Cyclopropylamine
KW  - Immunosensor
KW  - Label-free detection
KW  - Plasma polymerization
KW  - Quartz crystal microbalance
KW  - Stable amine-rich films
KW  - Surface plasmon resonance
N2  - Immunosensors with highly sensitive and rapid detection capabilities of various biomolecules are of a great demand in the field of biomedicine and environmental control. The two mostly employed transducer principles are either optical surface plasmon resonance (SPR) and mass sensitive quartz crystal microbalance (QCM). The first task encountered in the immunosensor development is the preparation of a coating matrix suitable for the immobilization of antibody on the golden layer of SPR or QCM. The most popular approaches are based on wet chemical treatments such as self-assembled monolayers (SAMs) of alkanethiols, disulfides, or polyethylenimine (PEI). However, these approaches suffer from several drawbacks such as a poor stability, long time preparation, unstable baseline or a high level of noise. As an alternative to SAMs, the deposition of thin functional coatings by plasma polymerization can be employed. The plasma polymerization has already been successfully applied to the deposition of thin films containing carboxyl, amine, anhydride groups. In this work, pulsed plasma polymerization of cyclopropylamine is employed to deposit stable amine-rich thin films on the surfaces of QCM and SPR chips. The AL-01 antibody, specific to human serum albumin (HSA), was attached to the QCM and SPR chips surfaces via glutaraldehyde activation. For the developed immunosensors the stable baseline was recorded. Selective and high response was achieved during the reaction with the solution of antigen. The results confirmed that the introduced methodology have a great potential for biosensing applications.
ER  -

MAKHNEVA, Ekaterina, Zdeněk FARKA, Petr SKLÁDAL a Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing. In \textit{8th International Conference on Nanomaterials - Research and Application, NANOCON 2016}. Brno: TANGER Ltd., 2017, s.~395-401. ISBN~978-80-87294-71-0.

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