Cyclopropylamine plasma polymer surfaces in quartz crystal
microbalance and surface plasmon resonance immunosensing

D 2017

Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing

MAKHNEVA, Ekaterina; Zdeněk FARKA; Petr SKLÁDAL and Lenka ZAJÍČKOVÁ

Basic information

Original name

Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing

Authors

MAKHNEVA, Ekaterina (643 Russian Federation, belonging to the institution); Zdeněk FARKA (203 Czech Republic, belonging to the institution); Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Brno, 8th International Conference on Nanomaterials - Research and Application, NANOCON 2016, p. 395-401, 7 pp. 2017

Publisher

TANGER Ltd.

Other information

Language

English

Type of outcome

Proceedings paper

Field of Study

10305 Fluids and plasma physics

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

Publication form

storage medium (CD, DVD, flash disk)

RIV identification code

RIV/00216224:14740/17:00096777

Organization unit

Central European Institute of Technology

ISBN

978-80-87294-71-0

UT WoS

000410656100069

Keywords in English

Cyclopropylamine; Immunosensor; Label-free detection; Plasma polymerization; Quartz crystal microbalance; Stable amine-rich films; Surface plasmon resonance

Abstract

In the original language

Immunosensors with highly sensitive and rapid detection capabilities of various biomolecules are of a great demand in the field of biomedicine and environmental control. The two mostly employed transducer principles are either optical surface plasmon resonance (SPR) and mass sensitive quartz crystal microbalance (QCM). The first task encountered in the immunosensor development is the preparation of a coating matrix suitable for the immobilization of antibody on the golden layer of SPR or QCM. The most popular approaches are based on wet chemical treatments such as self-assembled monolayers (SAMs) of alkanethiols, disulfides, or polyethylenimine (PEI). However, these approaches suffer from several drawbacks such as a poor stability, long time preparation, unstable baseline or a high level of noise. As an alternative to SAMs, the deposition of thin functional coatings by plasma polymerization can be employed. The plasma polymerization has already been successfully applied to the deposition of thin films containing carboxyl, amine, anhydride groups. In this work, pulsed plasma polymerization of cyclopropylamine is employed to deposit stable amine-rich thin films on the surfaces of QCM and SPR chips. The AL-01 antibody, specific to human serum albumin (HSA), was attached to the QCM and SPR chips surfaces via glutaraldehyde activation. For the developed immunosensors the stable baseline was recorded. Selective and high response was achieved during the reaction with the solution of antigen. The results confirmed that the introduced methodology have a great potential for biosensing applications.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Cite

MAKHNEVA, Ekaterina; Zdeněk FARKA; Petr SKLÁDAL and Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing. In 8th International Conference on Nanomaterials - Research and Application, NANOCON 2016. Brno: TANGER Ltd., 2017, p. 395-401. ISBN 978-80-87294-71-0.

@inproceedings{1381386,
   author = {Makhneva, Ekaterina and Farka, Zdeněk and Skládal, Petr and Zajíčková, Lenka},
   address = {Brno},
   booktitle = {8th International Conference on Nanomaterials - Research and Application, NANOCON 2016},
   keywords = {Cyclopropylamine; Immunosensor; Label-free detection; Plasma polymerization; Quartz crystal microbalance; Stable amine-rich films; Surface plasmon resonance},
   howpublished = {paměťový nosič},
   language = {eng},
   location = {Brno},
   isbn = {978-80-87294-71-0},
   pages = {395-401},
   publisher = {TANGER Ltd.},
   title = {Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing},
   year = {2017}
}

TY  - CONF
ID  - 1381386
AU  - Makhneva, Ekaterina - Farka, Zdeněk - Skládal, Petr - Zajíčková, Lenka
PY  - 2017
TI  - Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing
PB  - TANGER Ltd.
CY  - Brno
SN  - 9788087294710
KW  - Cyclopropylamine
KW  - Immunosensor
KW  - Label-free detection
KW  - Plasma polymerization
KW  - Quartz crystal microbalance
KW  - Stable amine-rich films
KW  - Surface plasmon resonance
N2  - Immunosensors with highly sensitive and rapid detection capabilities of various biomolecules are of a great demand in the field of biomedicine and environmental control. The two mostly employed transducer principles are either optical surface plasmon resonance (SPR) and mass sensitive quartz crystal microbalance (QCM). The first task encountered in the immunosensor development is the preparation of a coating matrix suitable for the immobilization of antibody on the golden layer of SPR or QCM. The most popular approaches are based on wet chemical treatments such as self-assembled monolayers (SAMs) of alkanethiols, disulfides, or polyethylenimine (PEI). However, these approaches suffer from several drawbacks such as a poor stability, long time preparation, unstable baseline or a high level of noise. As an alternative to SAMs, the deposition of thin functional coatings by plasma polymerization can be employed. The plasma polymerization has already been successfully applied to the deposition of thin films containing carboxyl, amine, anhydride groups. In this work, pulsed plasma polymerization of cyclopropylamine is employed to deposit stable amine-rich thin films on the surfaces of QCM and SPR chips. The AL-01 antibody, specific to human serum albumin (HSA), was attached to the QCM and SPR chips surfaces via glutaraldehyde activation. For the developed immunosensors the stable baseline was recorded. Selective and high response was achieved during the reaction with the solution of antigen. The results confirmed that the introduced methodology have a great potential for biosensing applications.
ER  -

MAKHNEVA, Ekaterina; Zdeněk FARKA; Petr SKLÁDAL and Lenka ZAJÍČKOVÁ. Cyclopropylamine plasma polymer surfaces in quartz crystal microbalance and surface plasmon resonance immunosensing. In \textit{8th International Conference on Nanomaterials - Research and Application, NANOCON 2016}. Brno: TANGER Ltd., 2017, p.~395-401. ISBN~978-80-87294-71-0.

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