2017
Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging
KHAIRNAR, Amit Suresh, Jana RUDÁ, Nikoletta SZABÓ, Eva DRAŽANOVÁ, Anas ARAB et. al.Základní údaje
Originální název
Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging
Autoři
KHAIRNAR, Amit Suresh (356 Indie, domácí), Jana RUDÁ (203 Česká republika, domácí), Nikoletta SZABÓ (348 Maďarsko), Eva DRAŽANOVÁ (203 Česká republika, domácí), Anas ARAB (760 Sýrie, domácí), Birgit HUTTER-PAIER (40 Rakousko), Joerg NEDDENS (40 Rakousko), Peter LATTA (703 Slovensko, domácí), Zenon STARČUK (203 Česká republika, domácí) a Irena REKTOROVÁ (203 Česká republika, garant, domácí)
Vydání
Brain, Behavior, and Immunity, San Diego, Academic Press, 2017, 0889-1591
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30102 Immunology
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 6.306
Kód RIV
RIV/00216224:14740/17:00096844
Organizační jednotka
Středoevropský technologický institut
UT WoS
000395365900022
Klíčová slova anglicky
MRI; Diffusion kurtosis imaging; Substantia nigra; Striatum; Thalamus; TNWT-61; Parkinson's disease; Transgenic mice; Animal model
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 26. 2. 2018 11:11, Mgr. Pavla Foltynová, Ph.D.
Anotace
V originále
Diffusion kurtosis imaging (DKI) is sensitive in detecting alpha-Synuclein (alpha-Syn) accumulation-associated microstructural changes at late stages of the pathology in alpha-Syn overexpressing TNWT-61 mice. The aim of this study was to perform DKI in young TNWT-61 mice when alpha-Syn starts to accumulate and to compare the imaging results with an analysis of motor and memory impairment and alpha-Syn levels. Three-month-old (3mo) and six-month-old (6mo) mice underwent DIU scanning using the Bruker Avance 9.4 T magnetic resonance imaging system. Region of interest (ROI) analyses were performed in the gray matter; tract-based spatial statistics (TBSS) analyses were performed in the white matter. In the same mice, alpha-Syn expression was evaluated using quantitative immunofluorescence. Mean kurtosis (MK) was the best differentiator between TNWT-61 mice and wildtype (WT) mice. We found increases in MK in 3mo TNWT-61 mice in the striatum and thalamus but not in the substantia nigra (SN), hippocampus, or sensorimotor cortex, even though the immunoreactivity of human alpha-Syn was similar or even higher in the latter regions. Increases in MK in the SN were detected in 6mo mice. These findings indicate that alpha-Syn accumulation-associated changes may start in areas with a high density of dopaminergic nerve terminals. We also found TBSS changes in white matter only at 6mo, suggesting alpha-Syn accumulation-associated changes start in the gray matter and later progress to the white matter. MK is able to detect microstructural changes induced by alpha-Syn overexpression in TNWT-61 mice and could be a useful clinical tool for detecting early-stage Parkinson's disease in human patients. (C) 2016 Elsevier Inc. All rights reserved.
Návaznosti
LM2015062, projekt VaV |
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LQ1601, projekt VaV |
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MUNI/A/1063/2016, interní kód MU |
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