TŘÍSKOVÁ, Iveta, Markéta BOSÁKOVÁ and Libuše TRNKOVÁ. PENCIL GRAPHITE ELECTRODE AS A PROMISING TOOL FOR ELECTROANALYSIS OF METHYLGUANINES. In Libuše Trnková. XVII. Workshop of Physical Chemists and Electrochemists. 1st ed. Brno: Masarykova univerzita. p. 50-51. ISBN 978-80-210-8580-0. 2017.
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Basic information
Original name PENCIL GRAPHITE ELECTRODE AS A PROMISING TOOL FOR ELECTROANALYSIS OF METHYLGUANINES
Authors TŘÍSKOVÁ, Iveta, Markéta BOSÁKOVÁ and Libuše TRNKOVÁ.
Edition 1. vyd. Brno, XVII. Workshop of Physical Chemists and Electrochemists, p. 50-51, 2 pp. 2017.
Publisher Masarykova univerzita
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10403 Physical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
Organization unit Faculty of Science
ISBN 978-80-210-8580-0
Keywords in English epigenetic modifications; DNA methylation; methylguanines; voltammetry; PeGE
Tags NZ
Changed by Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 24/3/2018 14:03.
Abstract
Nowadays, epigenetics is among the top disciplines of biomedicine. It is actually because epigentic modifications, especially methylation of nucleic acids (NAs), are linked with many diseases and dysfunctions, such as human carcinomas (lung, thyroid, prostate and pancreas), leukemia, etc.[1-4]. Guanine (G), one of the two essential nucleic bases, plays a key role in the DNA oxidation by various types of oxidants and free radicals [5-7]. Although guanine, compared with cytosine, is not a typical recipient of methyl groups, methylated guanines attract the attention as potential drugs and/or markers. Specifically, N7-methyl modification of G is a well-established biomarker for the detection and determination of DNA methylation [8]. Only few examples can be found in the literature regarding to the electrochemical determination of this alkylmodification of G. The electrochemical reduction of 7-methylguanine derivatives (7-methylguanosine and 7-methylguanosine-5‘-phosphate) at the mercury electrode [3, 9, 10] was completed with the study of oxidation behavior of 7-methylguanine at boron-doped diamond electrode and carbon electrodes (glassy carbon electrode and screen printed graphite electrodes)[8]. There are some papers regarding electrochemical behavior of 7N-methylguanine (7N-mG), linked with epigenetic modifications of DNA chain, but there are not any publications regarding other methylated guanine derivatives, such as 1N-, 3N- or 9N-methylguanines. In our research the oxidation processes of methylated guanine derivatives on a polymer pencil graphite electrode (pPeGE) in dependence on pH (phosphate – acetate buffer; pH 3.16 – 7.52) and the position of methyl group in the G molecule (pyrimidine ring vs. imidazole ring) were investigated.
Links
MUNI/A/1237/2016, interní kód MUName: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017
Investor: Masaryk University, Category A
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