The Oxidation Processes of Methylguanines on a Pencil Graphite
Electrode

D 2017

The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode

BOSÁKOVÁ, Markéta, Iveta TŘÍSKOVÁ, Jan HRBÁČ a Libuše TRNKOVÁ

Základní údaje

Originální název

The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode

Autoři

BOSÁKOVÁ, Markéta (203 Česká republika, domácí), Iveta TŘÍSKOVÁ (203 Česká republika, domácí), Jan HRBÁČ (203 Česká republika, domácí) a Libuše TRNKOVÁ (203 Česká republika, garant, domácí)

Vydání

1. vyd. Ústí nad Labem, XXXVII. Moderní elektrochemické metody, od s. 19-22, 4 s. 2017

Nakladatel

Srsenová Lenka - BEST servis Ústí nad Labem

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10403 Physical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14310/17:00096907

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-905221-5-2

UT WoS

000432424300003

Klíčová slova anglicky

guanine; methylguanines; epigenetic modifications; DNA methylation; voltammetry; pencil graphite electrode

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2020 14:43, Mgr. Marie Šípková, DiS.

Anotace

V originále

In the last time, epigenetics is among top disciplines in the field of medicine research because epigenetic modifications of nucleic acids are associated with certain diseases and dysfunctions (e.g. human carcinomas – lung, thyroid, prostate and pancreas tumors-, leukemia, etc.). One of several epigenetic mechanisms that cells use to control gene expression is DNA methylation and one of different methylated guanines, N7- methyl G is recognized as the biomarker of DNA methylation. In the present study, electrochemical oxidation of methylated guanines (1N-, 7N- and 9N- derivatives) is studied to ascertain the relationship between the position of methyl group and the corresponding oxidation behavior. For this reason, the oxidation processes of methylguanines (methylGs) in the dependence on pH and the position of methyl group in the guanine molecule (pyrimidine ring vs. imidazole ring) were investigated. It was found that methylation has significant influence on the oxidation processes of methylGs and their oxidation is easier in the case of CH3- substitution at the pyrimidine ring than at imidazole one. Pencil graphite electrode (PeGE) was used as a working electrode throughout the study, since voltammetric signals of methylGs were poorly defined at conventional carbon electrodes, such as glassy carbon or HOPG.

Návaznosti

MUNI/A/1237/2016, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

BOSÁKOVÁ, Markéta, Iveta TŘÍSKOVÁ, Jan HRBÁČ a Libuše TRNKOVÁ. The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode. In Tomáš Navrátil, Miroslav Fojta, Karolina Schwarzová. XXXVII. Moderní elektrochemické metody. 1. vyd. Ústí nad Labem: Srsenová Lenka - BEST servis Ústí nad Labem, 2017, s. 19-22. ISBN 978-80-905221-5-2.

@inproceedings{1382530,
   author = {Bosáková, Markéta and Třísková, Iveta and Hrbáč, Jan and Trnková, Libuše},
   address = {Ústí nad Labem},
   booktitle = {XXXVII. Moderní elektrochemické metody},
   edition = {1},
   editor = {Tomáš Navrátil, Miroslav Fojta, Karolina Schwarzová},
   keywords = {guanine; methylguanines; epigenetic modifications; DNA methylation; voltammetry; pencil graphite electrode},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Ústí nad Labem},
   isbn = {978-80-905221-5-2},
   pages = {19-22},
   publisher = {Srsenová Lenka - BEST servis Ústí nad Labem},
   title = {The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode},
   url = {https://www.bestservis.eu/upload/file/Sbornik_metody17.pdf},
   year = {2017}
}

TY  - JOUR
ID  - 1382530
AU  - Bosáková, Markéta - Třísková, Iveta - Hrbáč, Jan - Trnková, Libuše
PY  - 2017
TI  - The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode
PB  - Srsenová Lenka - BEST servis Ústí nad Labem
CY  - Ústí nad Labem
SN  - 9788090522152
KW  - guanine
KW  - methylguanines
KW  - epigenetic modifications
KW  - DNA methylation
KW  - voltammetry
KW  - pencil graphite electrode
UR  - https://www.bestservis.eu/upload/file/Sbornik_metody17.pdf
L2  - https://www.bestservis.eu/upload/file/Sbornik_metody17.pdf
N2  - In the last time, epigenetics is among top disciplines in the field of medicine research because epigenetic modifications of nucleic acids are associated with certain diseases and dysfunctions (e.g. human carcinomas – lung, thyroid, prostate and pancreas tumors-, leukemia, etc.). One of several epigenetic mechanisms that cells use to control gene expression is DNA methylation and one of different methylated guanines, N7- methyl G is recognized as the biomarker of DNA methylation. In the present study, electrochemical oxidation of methylated guanines (1N-, 7N- and 9N- derivatives) is studied to ascertain the relationship between the position of methyl group and the corresponding oxidation behavior. For this reason, the oxidation processes of methylguanines (methylGs) in the dependence on pH and the position of methyl group in the guanine molecule (pyrimidine ring vs. imidazole ring) were investigated. It was found that methylation has significant influence on the oxidation processes of methylGs and their oxidation is easier in the case of CH3- substitution at the pyrimidine ring than at imidazole one. Pencil graphite electrode (PeGE) was used as a working electrode throughout the study, since voltammetric signals of methylGs were poorly defined at conventional carbon electrodes, such as glassy carbon or HOPG.
ER  -

BOSÁKOVÁ, Markéta, Iveta TŘÍSKOVÁ, Jan HRBÁČ a Libuše TRNKOVÁ. The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode. In Tomáš Navrátil, Miroslav Fojta, Karolina Schwarzová. \textit{XXXVII. Moderní elektrochemické metody}. 1. vyd. Ústí nad Labem: Srsenová Lenka - BEST servis Ústí nad Labem, 2017, s.~19-22. ISBN~978-80-905221-5-2.

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