Structure of a Stable G-Hairpin

J 2017

Structure of a Stable G-Hairpin

GAJARSKÝ, Martin; Martina Lenarcic ZIVKOVIC; Petr STADLBAUER; Bruno PAGANO; Radovan FIALA et. al.

Základní údaje

Originální název

Structure of a Stable G-Hairpin

Autoři

GAJARSKÝ, Martin (703 Slovensko, domácí); Martina Lenarcic ZIVKOVIC (705 Slovinsko); Petr STADLBAUER (203 Česká republika); Bruno PAGANO (380 Itálie); Radovan FIALA (203 Česká republika, domácí); Jussara AMATO (380 Itálie); L´ubomir TOMASKA (703 Slovensko); Jiří ŠPONER (203 Česká republika, domácí); Janez PLAVEC (705 Slovinsko) a Lukáš TRANTÍREK (203 Česká republika, garant, domácí)

Vydání

Journal of the American Chemical Society, WASHINGTON, American Chemical Society, 2017, 0002-7863

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 14.357

Kód RIV

RIV/00216224:14740/17:00094788

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1021/jacs.6b10786

UT WoS

000396801300002

EID Scopus

2-s2.0-85015171725

Klíčová slova anglicky

G-QUADRUPLEX STRUCTURES; HUMAN TELOMERIC DNA; SINGLE-STRANDED-DNA; G-TRIPLEX; FOLDING PATHWAYS; KINETICS; RECOGNITION; VISUALIZATION; DETERMINANTS; REPEATS

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 6. 2017 14:06, Mgr. Eva Špillingová

Anotace

V originále

In this study, we report the first atomic resolution structure of a stable G-hairpin formed by a natively occurring DNA sequence. An 11-nt long G-rich DNA oligonucleotide, 5'-d(GTGTGGGTGTG)-3', corresponding to the most abundant sequence motif in irregular telomeric DNA from Saccharomyces cerevisiae (yeast), is demonstrated to adopt a novel type of mixed parallel/ antiparallel fold-back DNA structure, which is stabilized by dynamic G:G base pairs that transit between NI -carbonyl symmetric and N1-carbonyl, N7-aminobase-pairingarrangements. Although the studied sequence first appears to possess a low capacity for base pairing, it forms a thermodynamically stable structure with a rather complex topology that includes a chain reversal arrangement of the backbone in the center of the continuous G-tract and 3' to -5' stacking of the terminal residues. The structure reveals previously unknown principles of the folding of G rich oligonucleotides that could be applied to the prediction of natural and/or the design of artificial recognition DNA elements. The structure also demonstrates that the folding landscapes of short DNA single strands is much more complex than previously assumed.

Návaznosti

GA13-28310S, projekt VaV

Název: Evolučně konzervované strukturní vlastnosti centromerické a telomerické DNA

Investor: Grantová agentura ČR, Evolucne konzervované strukturní vlastnosti centromerické a telomerické DNA

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

GAJARSKÝ, Martin; Martina Lenarcic ZIVKOVIC; Petr STADLBAUER; Bruno PAGANO; Radovan FIALA; Jussara AMATO; L´ubomir TOMASKA; Jiří ŠPONER; Janez PLAVEC a Lukáš TRANTÍREK. Structure of a Stable G-Hairpin. Journal of the American Chemical Society. WASHINGTON: American Chemical Society, 2017, roč. 139, č. 10, s. 3591-3594. ISSN 0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.6b10786.

@article{1383183,
   author = {Gajarský, Martin and Zivkovic, Martina Lenarcic and Stadlbauer, Petr and Pagano, Bruno and Fiala, Radovan and Amato, Jussara and Tomaska, L´ubomir and Šponer, Jiří and Plavec, Janez and Trantírek, Lukáš},
   article_location = {WASHINGTON},
   article_number = {10},
   doi = {http://dx.doi.org/10.1021/jacs.6b10786},
   keywords = {G-QUADRUPLEX STRUCTURES; HUMAN TELOMERIC DNA; SINGLE-STRANDED-DNA; G-TRIPLEX; FOLDING PATHWAYS; KINETICS; RECOGNITION; VISUALIZATION; DETERMINANTS; REPEATS},
   language = {eng},
   issn = {0002-7863},
   journal = {Journal of the American Chemical Society},
   title = {Structure of a Stable G-Hairpin},
   url = {http://pubs.acs.org/doi/abs/10.1021/jacs.6b10786},
   volume = {139},
   year = {2017}
}

TY  - JOUR
ID  - 1383183
AU  - Gajarský, Martin - Zivkovic, Martina Lenarcic - Stadlbauer, Petr - Pagano, Bruno - Fiala, Radovan - Amato, Jussara - Tomaska, L´ubomir - Šponer, Jiří - Plavec, Janez - Trantírek, Lukáš
PY  - 2017
TI  - Structure of a Stable G-Hairpin
JF  - Journal of the American Chemical Society
VL  - 139
IS  - 10
SP  - 3591-3594
EP  - 3591-3594
PB  - American Chemical Society
SN  - 00027863
KW  - G-QUADRUPLEX STRUCTURES
KW  - HUMAN TELOMERIC DNA
KW  - SINGLE-STRANDED-DNA
KW  - G-TRIPLEX
KW  - FOLDING PATHWAYS
KW  - KINETICS
KW  - RECOGNITION
KW  - VISUALIZATION
KW  - DETERMINANTS
KW  - REPEATS
UR  - http://pubs.acs.org/doi/abs/10.1021/jacs.6b10786
L2  - http://pubs.acs.org/doi/abs/10.1021/jacs.6b10786
N2  - In this study, we report the first atomic resolution structure of a stable G-hairpin formed by a natively occurring DNA sequence. An 11-nt long G-rich DNA oligonucleotide, 5'-d(GTGTGGGTGTG)-3', corresponding to the most abundant sequence motif in irregular telomeric DNA from Saccharomyces cerevisiae (yeast), is demonstrated to adopt a novel type of mixed parallel/ antiparallel fold-back DNA structure, which is stabilized by dynamic G:G base pairs that transit between NI -carbonyl symmetric and N1-carbonyl, N7-aminobase-pairingarrangements. Although the studied sequence first appears to possess a low capacity for base pairing, it forms a thermodynamically stable structure with a rather complex topology that includes a chain reversal arrangement of the backbone in the center of the continuous G-tract and 3' to -5' stacking of the terminal residues. The structure reveals previously unknown principles of the folding of G rich oligonucleotides that could be applied to the prediction of natural and/or the design of artificial recognition DNA elements. The structure also demonstrates that the folding landscapes of short DNA single strands is much more complex than previously assumed.
ER  -

GAJARSKÝ, Martin; Martina Lenarcic ZIVKOVIC; Petr STADLBAUER; Bruno PAGANO; Radovan FIALA; Jussara AMATO; L´ubomir TOMASKA; Jiří ŠPONER; Janez PLAVEC a Lukáš TRANTÍREK. Structure of a Stable G-Hairpin. \textit{Journal of the American Chemical Society}. WASHINGTON: American Chemical Society, 2017, roč.~139, č.~10, s.~3591-3594. ISSN~0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.6b10786.

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