Evaluation of sample preparation protocols for spider venom
profiling by MALDI-TOF MS

J 2017

Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS

BOČÁNEK, Ondřej, Ondrej ŠEDO, Stanislav PEKÁR a Zbyněk ZDRÁHAL

Základní údaje

Originální název

Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS

Autoři

BOČÁNEK, Ondřej (203 Česká republika, domácí), Ondrej ŠEDO (203 Česká republika, domácí), Stanislav PEKÁR (703 Slovensko, garant, domácí) a Zbyněk ZDRÁHAL (203 Česká republika, domácí)

Vydání

Toxicon, Oxford, England, PERGAMON-ELSEVIER SCIENCE LTD, 2017, 0041-0101

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.352

Kód RIV

RIV/00216224:14310/17:00094825

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.toxicon.2017.04.008

UT WoS

000403864000003

Klíčová slova anglicky

Venom mass fingerprinting; Spider venom; MALDI-TOF MS

Štítky

CF PROT, NZ, rivok

Změněno: 3. 4. 2018 14:56, Ing. Nicole Zrilić

Anotace

V originále

Spider venoms are highly complex mixtures containing biologically active substances with potential for use in biotechnology or pharmacology. Fingerprinting of venoms by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) is a thriving technology, enabling the rapid detection of peptide/protein components that can provide comparative information. In this study, we evaluated the effects of sample preparation procedures on MALDI-TOF mass spectral quality to establish a protocol providing the most reliable analytical outputs. We adopted initial sample preparation conditions from studies already published in this field. Three different MALDI matrixes, three matrix solvents, two sample deposition methods, and different acid concentrations were tested. As a model sample, venom from Brachypelma albopilosa was used. The mass spectra were evaluated on the basis of absolute and relative signal intensities, and signal resolution. By conducting three series of analyses at three weekly intervals, the reproducibility of the mass spectra were assessed as a crucial factor in the selection for optimum conditions. A sample preparation protocol based on the use of an HCCA matrix dissolved in 50% acetonitrile with 2.5% TFA deposited onto the target by the dried-droplet method was found to provide the best results in terms of information yield and repeatability. We propose that this protocol should be followed as a standard procedure, enabling the comparative assessment of MALDI-TOF MS spider venom fingerprints.

Návaznosti

GA15-14762S, projekt VaV

Název: Koevoluce potravní-specializace a složení jedu u pavouků

Investor: Grantová agentura ČR, Koevoluce potravní-specializace a složení jedu u pavouků

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

BOČÁNEK, Ondřej, Ondrej ŠEDO, Stanislav PEKÁR a Zbyněk ZDRÁHAL. Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS. Toxicon. Oxford, England: PERGAMON-ELSEVIER SCIENCE LTD, 2017, roč. 133, July, s. 18-25. ISSN 0041-0101. Dostupné z: https://dx.doi.org/10.1016/j.toxicon.2017.04.008.

@article{1384857,
   author = {Bočánek, Ondřej and Šedo, Ondrej and Pekár, Stanislav and Zdráhal, Zbyněk},
   article_location = {Oxford, England},
   article_number = {July},
   doi = {http://dx.doi.org/10.1016/j.toxicon.2017.04.008},
   keywords = {Venom mass fingerprinting; Spider venom; MALDI-TOF MS},
   language = {eng},
   issn = {0041-0101},
   journal = {Toxicon},
   title = {Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS},
   volume = {133},
   year = {2017}
}

TY  - JOUR
ID  - 1384857
AU  - Bočánek, Ondřej - Šedo, Ondrej - Pekár, Stanislav - Zdráhal, Zbyněk
PY  - 2017
TI  - Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS
JF  - Toxicon
VL  - 133
IS  - July
SP  - 18-25
EP  - 18-25
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00410101
KW  - Venom mass fingerprinting
KW  - Spider venom
KW  - MALDI-TOF MS
N2  - Spider venoms are highly complex mixtures containing biologically active substances with potential for use in biotechnology or pharmacology. Fingerprinting of venoms by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) is a thriving technology, enabling the rapid detection of peptide/protein components that can provide comparative information. In this study, we evaluated the effects of sample preparation procedures on MALDI-TOF mass spectral quality to establish a protocol providing the most reliable analytical outputs. We adopted initial sample preparation conditions from studies already published in this field. Three different MALDI matrixes, three matrix solvents, two sample deposition methods, and different acid concentrations were tested. As a model sample, venom from Brachypelma albopilosa was used. The mass spectra were evaluated on the basis of absolute and relative signal intensities, and signal resolution. By conducting three series of analyses at three weekly intervals, the reproducibility of the mass spectra were assessed as a crucial factor in the selection for optimum conditions. A sample preparation protocol based on the use of an HCCA matrix dissolved in 50% acetonitrile with 2.5% TFA deposited onto the target by the dried-droplet method was found to provide the best results in terms of information yield and repeatability. We propose that this protocol should be followed as a standard procedure, enabling the comparative assessment of MALDI-TOF MS spider venom fingerprints.
ER  -

BOČÁNEK, Ondřej, Ondrej ŠEDO, Stanislav PEKÁR a Zbyněk ZDRÁHAL. Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS. \textit{Toxicon}. Oxford, England: PERGAMON-ELSEVIER SCIENCE LTD, 2017, roč.~133, July, s.~18-25. ISSN~0041-0101. Dostupné z: https://dx.doi.org/10.1016/j.toxicon.2017.04.008.

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