Characterization of two highly divergent lineages of
exfoliative toxin B-encoding plasmids revealed in impetigo
strains of Staphylococcus aureus

D 2017

Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ et. al.

Základní údaje

Originální název

Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus

Autoři

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ, Darina ČEJKOVÁ a Jiří DOŠKAŘ

Vydání

Germany, EMBO | EMBL Symposium: New Approaches and Concepts in Microbiology, s. 122-122, 2017

Nakladatel

EMBL Heidelberg, Germany

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

ETB plazmid; exfoliativní toxin B; fylogeneze; genomová analýza

Klíčová slova anglicky

ETB plasmid; exfoliative toxin B; phylogeny; genome analysis

Štítky

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 4. 2018 14:48, Ing. Nicole Zrilić

Anotace

V originále

Staphylococcal epidermolytic diseases are caused by S. aureus strains producing exfoliative toxins A and B encoded by mobile genetic elements: plasmids and prophages. We analyzed various etb gene-positive plasmids isolated from the impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Most of the plasmids were related to archetypal plasmid pETBTY4. They shared a same core sequence, but they differed from each other in the content of additional DNA regions such as antibiotic resistance-encoding transposons. The ETB plasmids of this lineage were carried by strains of clonal complex CC121. Some of these strains also harbored exfoliative toxin A-encoding prophages. A novel distinct type of ETB plasmid originated from a prophageless impetigo strain was isolated and characterized in detail. Carrying conjugative cluster genes, as well as new variants of ETB and EDIN genes, it could be regarded as a source of a new lineage of ETB plasmids. Based on our findings, we have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.

Návaznosti

QJ1510216, projekt VaV

Název: Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat (Akronym: StafyFag)

Investor: Ministerstvo zemědělství ČR, Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat

Citovat

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ, Darina ČEJKOVÁ a Jiří DOŠKAŘ. Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus. In Carolina Garcia Sabaté. EMBO | EMBL Symposium: New Approaches and Concepts in Microbiology. Germany: EMBL Heidelberg, Germany, 2017, s. 122.

@inproceedings{1385022,
   author = {Botka, Tibor and Růžičková, Vladislava and Svobodová, Karla and Pantůček, Roman and Petráš, Petr and Čejková, Darina and Doškař, Jiří},
   address = {Germany},
   booktitle = {EMBO | EMBL Symposium: New Approaches and Concepts in Microbiology},
   editor = {Carolina Garcia Sabaté},
   keywords = {ETB plasmid; exfoliative toxin B; phylogeny; genome analysis},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Germany},
   note = {Nepřenášíme do RIV, výsledek má jen 1 stranu, podle metodiky M17+ musí mít stať ve sborníku min. 2 strany.},
   pages = {122-122},
   publisher = {EMBL Heidelberg, Germany},
   title = {Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus},
   url = {https://www.embo-embl-symposia.org/symposia/2017/EES17-05/index.html},
   year = {2017}
}

TY  - JOUR
ID  - 1385022
AU  - Botka, Tibor - Růžičková, Vladislava - Svobodová, Karla - Pantůček, Roman - Petráš, Petr - Čejková, Darina - Doškař, Jiří
PY  - 2017
TI  - Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus
PB  - EMBL Heidelberg, Germany
CY  - Germany
N1  - Nepřenášíme do RIV, výsledek má jen 1 stranu, podle metodiky M17+ musí mít stať ve sborníku min. 2 strany.
KW  - ETB plasmid
KW  - exfoliative toxin B
KW  - phylogeny
KW  - genome analysis
UR  - https://www.embo-embl-symposia.org/symposia/2017/EES17-05/index.html
N2  - Staphylococcal epidermolytic diseases are caused by S. aureus strains producing exfoliative toxins A and B encoded by mobile genetic elements: plasmids and prophages. We analyzed various etb gene-positive plasmids isolated from the impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Most of the plasmids were related to archetypal plasmid pETBTY4. They shared a same core sequence, but they differed from each other in the content of additional DNA regions such as antibiotic resistance-encoding transposons. The ETB plasmids of this lineage were carried by strains of clonal complex CC121. Some of these strains also harbored exfoliative toxin A-encoding prophages. A novel distinct type of ETB plasmid originated from a prophageless impetigo strain was isolated and characterized in detail. Carrying conjugative cluster genes, as well as new variants of ETB and EDIN genes, it could be regarded as a source of a new lineage of ETB plasmids. Based on our findings, we have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.
ER  -

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ, Darina ČEJKOVÁ a Jiří DOŠKAŘ. Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus. In Carolina Garcia Sabaté. \textit{EMBO $|$ EMBL Symposium: New Approaches and Concepts in Microbiology}. Germany: EMBL Heidelberg, Germany, 2017, s.~122.

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