Motility in blastogregarines (Apicomplexa): Native and
drug-induced organisation of Siedleckia nematoides cytoskeletal
elements

J 2017

Motility in blastogregarines (Apicomplexa): Native and drug-induced organisation of Siedleckia nematoides cytoskeletal elements

BARDŮNEK VALIGUROVÁ, Andrea, Naděžda VAŠKOVICOVÁ, Andrei DIAKIN, Gita PASKEROVA, Timur G. SIMDYANOV et. al.

Základní údaje

Originální název

Motility in blastogregarines (Apicomplexa): Native and drug-induced organisation of Siedleckia nematoides cytoskeletal elements

Autoři

BARDŮNEK VALIGUROVÁ, Andrea (703 Slovensko, garant, domácí), Naděžda VAŠKOVICOVÁ (203 Česká republika), Andrei DIAKIN (643 Rusko, domácí), Gita PASKEROVA (643 Rusko), Timur G. SIMDYANOV (643 Rusko) a Magdaléna KOVÁČIKOVÁ (703 Slovensko, domácí)

Vydání

PLOS ONE, SAN FRANCISCO, Public Library of Science, 2017, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.766

Kód RIV

RIV/00216224:14310/17:00094844

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0179709

UT WoS

000404135800054

Klíčová slova anglicky

Actin; tubulin; drug-induced changes; cytoskeleton; motility; Siedleckia nematoides

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 4. 2018 14:46, Ing. Nicole Zrilić

Anotace

V originále

Recent studies on motility of Apicomplexa concur with the so-called glideosome concept applied for apicomplexan zoites, describing a unique mechanism of substrate-dependent gliding motility facilitated by a conserved form of actomyosin motor and subpellicular microtubules. In contrast, the gregarines and blastogregarines exhibit different modes and mechanisms of motility, correlating with diverse modifications of their cortex. This study focuses on the motility and cytoskeleton of the blastogregarine Siedleckia nematoides Caullery et Mesnil, 1898 parasitising the polychaete Scoloplos cf. armiger (Mu ¨ller, 1776). The blastogregarine moves independently on a solid substrate without any signs of gliding motility; the motility in a liquid environment (in both the attached and detached forms) rather resembles a sequence of pendular, twisting, undulation, and sometimes spasmodic movements. Despite the presence of key glideosome components such as pellicle consisting of the plasma membrane and the inner membrane complex, actin, myosin, subpellicular microtubules, micronemes and glycocalyx layer, the motility mechanism of S. nematoides differs from the glideosome machinery. Nevertheless, experimental assays using cytoskeletal probes proved that the polymerised forms of actin and tubulin play an essential role in the S. nematoides movement. Similar to Selenidium archigregarines, the subpellicular microtubules organised in several layers seem to be the leading motor structures in blastogregarine motility. The majority of the detected actin was stabilised in a polymerised form and appeared to be located beneath the inner membrane complex. The experimental data suggest the subpellicular microtubules to be associated with filamentous structures (= cross-linking protein complexes), presumably of actin nature.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

BARDŮNEK VALIGUROVÁ, Andrea, Naděžda VAŠKOVICOVÁ, Andrei DIAKIN, Gita PASKEROVA, Timur G. SIMDYANOV a Magdaléna KOVÁČIKOVÁ. Motility in blastogregarines (Apicomplexa): Native and drug-induced organisation of Siedleckia nematoides cytoskeletal elements. PLOS ONE. SAN FRANCISCO: Public Library of Science, 2017, roč. 12, č. 6, s. nestránkováno, 29 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0179709.

@article{1385941,
   author = {Bardůnek Valigurová, Andrea and Vaškovicová, Naděžda and Diakin, Andrei and Paskerova, Gita and Simdyanov, Timur G. and Kováčiková, Magdaléna},
   article_location = {SAN FRANCISCO},
   article_number = {6},
   doi = {http://dx.doi.org/10.1371/journal.pone.0179709},
   keywords = {Actin; tubulin; drug-induced changes; cytoskeleton; motility; Siedleckia nematoides},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {Motility in blastogregarines (Apicomplexa): Native and drug-induced organisation of Siedleckia nematoides cytoskeletal elements},
   url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0179709},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1385941
AU  - Bardůnek Valigurová, Andrea - Vaškovicová, Naděžda - Diakin, Andrei - Paskerova, Gita - Simdyanov, Timur G. - Kováčiková, Magdaléna
PY  - 2017
TI  - Motility in blastogregarines (Apicomplexa): Native and drug-induced organisation of Siedleckia nematoides cytoskeletal elements
JF  - PLOS ONE
VL  - 12
IS  - 6
SP  - nestránkováno
EP  - nestránkováno
PB  - Public Library of Science
SN  - 19326203
KW  - Actin
KW  - tubulin
KW  - drug-induced changes
KW  - cytoskeleton
KW  - motility
KW  - Siedleckia nematoides
UR  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0179709
L2  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0179709
N2  - Recent studies on motility of Apicomplexa concur with the so-called glideosome concept applied for apicomplexan zoites, describing a unique mechanism of substrate-dependent gliding motility facilitated by a conserved form of actomyosin motor and subpellicular microtubules. In contrast, the gregarines and blastogregarines exhibit different modes and mechanisms of motility, correlating with diverse modifications of their cortex. This study focuses on the motility and cytoskeleton of the blastogregarine Siedleckia nematoides Caullery et Mesnil, 1898 parasitising the polychaete Scoloplos cf. armiger (Mu ¨ller, 1776). The blastogregarine moves independently on a solid substrate without any signs of gliding motility; the motility in a liquid environment (in both the attached and detached forms) rather resembles a sequence of pendular, twisting, undulation, and sometimes spasmodic movements. Despite the presence of key glideosome components such as pellicle consisting of the plasma membrane and the inner membrane complex, actin, myosin, subpellicular microtubules, micronemes and glycocalyx layer, the motility mechanism of S. nematoides differs from the glideosome machinery. Nevertheless, experimental assays using cytoskeletal probes proved that the polymerised forms of actin and tubulin play an essential role in the S. nematoides movement. Similar to Selenidium archigregarines, the subpellicular microtubules organised in several layers seem to be the leading motor structures in blastogregarine motility. The majority of the detected actin was stabilised in a polymerised form and appeared to be located beneath the inner membrane complex. The experimental data suggest the subpellicular microtubules to be associated with filamentous structures (= cross-linking protein complexes), presumably of actin nature.
ER  -

BARDŮNEK VALIGUROVÁ, Andrea, Naděžda VAŠKOVICOVÁ, Andrei DIAKIN, Gita PASKEROVA, Timur G. SIMDYANOV a Magdaléna KOVÁČIKOVÁ. Motility in blastogregarines (Apicomplexa): Native and drug-induced organisation of Siedleckia nematoides cytoskeletal elements. \textit{PLOS ONE}. SAN FRANCISCO: Public Library of Science, 2017, roč.~12, č.~6, s.~nestránkováno, 29 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0179709.

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