Hematopoietic developmental potential of human pluripotent stem
cell lines is accompanied by the morphology of embryoid bodies
and the expression of endodermal and hematopoietic markers

J 2017

Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ

Základní údaje

Originální název

Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers

Autoři

TESAŘOVÁ, Lenka (203 Česká republika, domácí), Pavel ŠIMARA (203 Česká republika, domácí), Stanislav STEJSKAL (203 Česká republika, domácí) a Irena KRONTORÁD KOUTNÁ (203 Česká republika, garant, domácí)

Vydání

Cellular Reprogramming, 2017, 2152-4971

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.430

Kód RIV

RIV/00216224:14330/17:00094855

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1089/cell.2016.0042

UT WoS

000406526900007

Klíčová slova anglicky

pluripotent stem cells; hematopoietic differentiation; embryoid bodies; cytokines; hematopoietic stem cells

Štítky

cbia-web

Změněno: 27. 1. 2021 11:57, Mgr. Tereza Miškechová

Anotace

V originále

The potential clinical applications of hematopoietic stem cells derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid haematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available hPSC lines were differentiated via an embryoid body (EB) suspension culture in serum-free medium supplemented with three different cytokine mixes. The hPSC differentiation status was investigated by the flow cytometry expression profiles of cell surface molecules, and the gene expression of pluripotency and differentiation markers over time was evaluated by qRT-PCR. hPSC lines differed in several aspects of the differentiation process, including the absolute yield of hematopoietic progenitors, the proportion of hematopoietic progenitor populations and the effect of various cytokine mixes. The ability to generate hematopoietic progenitors was then associated with the morphology of the developing EBs and with the expression of the endodermal markers AFP and SOX17 and the hematopoietic transcription factor RUNX1. These findings deepen the knowledge about the hematopoietic propensity of hPSCs and identify its variability as an aspect that must be taken into account before the usage of hPSC-derived HSCs in downstream applications.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

LM2015090, projekt VaV

Název: Český národní uzel Evropské sítě infrastruktur klinického výzkumu (Akronym: CZECRIN)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CZECRIN - Český národní uzel Evropské sítě infrastruktur klinického výzkumu

Citovat

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers. Cellular Reprogramming. 2017, roč. 19, č. 4, s. 270-284. ISSN 2152-4971. Dostupné z: https://dx.doi.org/10.1089/cell.2016.0042.

@article{1386060,
   author = {Tesařová, Lenka and Šimara, Pavel and Stejskal, Stanislav and Krontorád Koutná, Irena},
   article_number = {4},
   doi = {http://dx.doi.org/10.1089/cell.2016.0042},
   keywords = {pluripotent stem cells; hematopoietic differentiation; embryoid bodies; cytokines; hematopoietic stem cells},
   language = {eng},
   issn = {2152-4971},
   journal = {Cellular Reprogramming},
   title = {Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers},
   url = {http://online.liebertpub.com/doi/10.1089/cell.2016.0042},
   volume = {19},
   year = {2017}
}

TY  - JOUR
ID  - 1386060
AU  - Tesařová, Lenka - Šimara, Pavel - Stejskal, Stanislav - Krontorád Koutná, Irena
PY  - 2017
TI  - Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers
JF  - Cellular Reprogramming
VL  - 19
IS  - 4
SP  - 270-284
EP  - 270-284
SN  - 21524971
KW  - pluripotent stem cells
KW  - hematopoietic differentiation
KW  - embryoid bodies
KW  - cytokines
KW  - hematopoietic stem cells
UR  - http://online.liebertpub.com/doi/10.1089/cell.2016.0042
L2  - http://online.liebertpub.com/doi/10.1089/cell.2016.0042
N2  - The potential clinical applications of hematopoietic stem cells derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid haematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available hPSC lines were differentiated via an embryoid body (EB) suspension culture in serum-free medium supplemented with three different cytokine mixes. The hPSC differentiation status was investigated by the flow cytometry expression profiles of cell surface molecules, and the gene expression of pluripotency and differentiation markers over time was evaluated by qRT-PCR. hPSC lines differed in several aspects of the differentiation process, including the absolute yield of hematopoietic progenitors, the proportion of hematopoietic progenitor populations and the effect of various cytokine mixes. The ability to generate hematopoietic progenitors was then associated with the morphology of the developing EBs and with the expression of the endodermal markers AFP and SOX17 and the hematopoietic transcription factor RUNX1. These findings deepen the knowledge about the hematopoietic propensity of hPSCs and identify its variability as an aspect that must be taken into account before the usage of hPSC-derived HSCs in downstream applications.
ER  -

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers. \textit{Cellular Reprogramming}. 2017, roč.~19, č.~4, s.~270-284. ISSN~2152-4971. Dostupné z: https://dx.doi.org/10.1089/cell.2016.0042.

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