2017
A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting
STREIT, Libor, Josef JAROŠ, Veronika SEDLÁKOVÁ, Miroslava SEDLÁČKOVÁ, Luboš DRAŽAN et. al.Základní údaje
Originální název
A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting
Autoři
STREIT, Libor (203 Česká republika, garant, domácí), Josef JAROŠ (203 Česká republika, domácí), Veronika SEDLÁKOVÁ (203 Česká republika, domácí), Miroslava SEDLÁČKOVÁ (203 Česká republika, domácí), Luboš DRAŽAN (203 Česká republika), Michal SVOBODA (203 Česká republika, domácí), Jakub POSPÍŠIL (203 Česká republika, domácí), Tomáš VÝŠKA (203 Česká republika), Jiří VESELÝ (203 Česká republika) a Aleš HAMPL (203 Česká republika, domácí)
Vydání
PLASTIC AND RECONSTRUCTIVE SURGERY, PHILADELPHIA, LIPPINCOTT WILLIAMS & WILKINS, 2017, 0032-1052
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30212 Surgery
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 3.621
Kód RIV
RIV/00216224:14110/17:00094856
Organizační jednotka
Lékařská fakulta
UT WoS
000395678200029
Klíčová slova anglicky
reconstructive surgery
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 15. 3. 2018 17:04, Soňa Böhmová
Anotace
V originále
Background: Lipomodeling is a technique that uses the patient's own fat for tissue regeneration and augmentation. The extent of regenerative effect is reported to be determined by the numbers of adipose-derived stem cells and the viability of cells in processed adipose tissue which, together with other factors, influence the degree of graft retention. This study addresses whether differences exist in properties of fat graft obtained by three commonly used techniques. Methods: Adipose tissue harvested from the hypogastric regions of 14 patients was processed by decantation, centrifugation, and membrane-based tissue filtration. The morphology of each preparation was assessed by electron microscopy and overall cell viability was assessed by live/dead assay. The number of adiposederived stem cells was determined and their stem cell character was assessed by the presence of cell surface molecules (i.e., CD105, CD90, CD31, and CD45) and by their capacity to differentiate into adipogenic and osteogenic lineages. Results: First, morphologies of processed fat samples obtained by individual procedures differed, but no preparation caused obvious damage to cellular or acellular components. Second, although the highest numbers of adiposederived stem cells were contained in the upper fraction of centrifuged lipoaspirates, the difference between preparations was marginal. Third, the maximal concentration of adipose fraction (removal of watery component) of lipoaspirate was achieved by membrane-based tissue filtration. Finally, no significant differences in overall viability were detected. Conclusions: Properties of processed lipoaspirate were influenced by the preparation procedure. However, the differences were not dramatic; both centrifugation and membrane-based filtration are methods of choice whose selection depends on other criteria (e.g., practicality) for individual surgical settings.
Návaznosti
ED1.1.00/02.0068, projekt VaV |
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EE2.3.20.0185, projekt VaV |
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GA16-02702S, projekt VaV |
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LD15144, projekt VaV |
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MUNI/A/1558/2014, interní kód MU |
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