Expression of Genes Encoding Zona Pellucida Glycoproteins and
Cortical Granule Distribution in Porcine Oocytes Isolated from
Small and Medium Follicles in Relation to Puberty Status of
Donors

J 2017

Expression of Genes Encoding Zona Pellucida Glycoproteins and Cortical Granule Distribution in Porcine Oocytes Isolated from Small and Medium Follicles in Relation to Puberty Status of Donors

JEŠETA, Michal, Joanna BUDNA, Wieslawa KRANC, Sarka HANULAKOVA, Artur BRYJA et. al.

Základní údaje

Originální název

Expression of Genes Encoding Zona Pellucida Glycoproteins and Cortical Granule Distribution in Porcine Oocytes Isolated from Small and Medium Follicles in Relation to Puberty Status of Donors

Autoři

JEŠETA, Michal (203 Česká republika, garant, domácí), Joanna BUDNA (616 Polsko), Wieslawa KRANC (616 Polsko), Sarka HANULAKOVA (203 Česká republika), Artur BRYJA (616 Polsko), Adrian CHACHULA (616 Polsko), Sylwia CIESIÓLKA (616 Polsko), Katarzyna WOJTANOWICZ-MARKIEWICZ (616 Polsko), Dorota BUKOWSKA (616 Polsko), Klaus-Peter BRUSSOW (616 Polsko), Malgorzata BRUSKA (616 Polsko), Michal NOWICKI (616 Polsko), Pawel ANTOSIK (616 Polsko), Pavlina HULINSKA (203 Česká republika), Marie MACHATKOVA (203 Česká republika), Maciej ZABEL (616 Polsko) a Bartosz KEMPISTY (616 Polsko)

Vydání

Czech Journal of Animal Science, Prague, Czech Academy Agricultural Sciences, 2017, 1212-1819

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40101 Agriculture

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.955

Kód RIV

RIV/00216224:14110/17:00097251

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.17221/90/2016-CJAS

UT WoS

000403547200002

Klíčová slova anglicky

pig; ovarian cycle; maturation; developmental competence; folliculogenesis

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2018 18:30, Soňa Böhmová

Anotace

V originále

The zona pellucida proteins belong to a group of proteins that regulate the processes of gamete recognition, interaction, and fusion, since they are recognized as primary and secondary sperm receptors. It is suggested that cortical granule distribution is significantly associated with the zona pellucida structure and membrane preparation to cortical and acrosome reaction. Therefore, this study investigated the zona pellucida marker gene expression (ZP2 and ZP4 protein) in relation to cortical granules distribution in oocytes and puberty status of donors. Oocytes were collected from adult cyclic sows (isolated from medium and small follicles) and juvenile gilts (isolated only from small follicles). The oocytes were examined by RT-qPCR and by confocal microscopy. The expression of genes for ZP2 and ZP4 protein in oocytes collected from small follicles from cycling sows was higher in comparison to oocytes from medium follicles or oocytes collected from small follicles from juvenile gilts (P < 0.001). We also observed increased expression of both ZP2 and ZP4 mRNA in oocytes collected from small follicles (juvenile gilts) as compared to medium follicles (cycling sows) (P < 0.001). Moreover, we found a difference in the distribution of cortical granules. In oocytes from medium follicles, the peripheral localization of cortical granules was twice higher than their central concentration. However, in oocytes derived from small follicles (cyclic sows) cortical granules in comparison to oocytes from medium follicles were more centrally localized (P < 0.05). It has been suggested that the donor puberty status and the size of follicles significantly influenced the zona pellucida gene expression and cortical granule localization in porcine oocytes. This is accompanied by fertilization specificity and fertilizability of porcine oocytes in vitro.

Návaznosti

ROZV/24/LF/2016, interní kód MU

Název: LF - Příspěvek IP 2016

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek IP 2016

Citovat

JEŠETA, Michal, Joanna BUDNA, Wieslawa KRANC, Sarka HANULAKOVA, Artur BRYJA, Adrian CHACHULA, Sylwia CIESIÓLKA, Katarzyna WOJTANOWICZ-MARKIEWICZ, Dorota BUKOWSKA, Klaus-Peter BRUSSOW, Malgorzata BRUSKA, Michal NOWICKI, Pawel ANTOSIK, Pavlina HULINSKA, Marie MACHATKOVA, Maciej ZABEL a Bartosz KEMPISTY. Expression of Genes Encoding Zona Pellucida Glycoproteins and Cortical Granule Distribution in Porcine Oocytes Isolated from Small and Medium Follicles in Relation to Puberty Status of Donors. Czech Journal of Animal Science. Prague: Czech Academy Agricultural Sciences, 2017, roč. 62, č. 6, s. 234-241. ISSN 1212-1819. Dostupné z: https://dx.doi.org/10.17221/90/2016-CJAS.

@article{1386376,
   author = {Ješeta, Michal and Budna, Joanna and Kranc, Wieslawa and Hanulakova, Sarka and Bryja, Artur and Chachula, Adrian and Ciesiólka, Sylwia and WojtanowiczandMarkiewicz, Katarzyna and Bukowska, Dorota and Brussow, KlausandPeter and Bruska, Malgorzata and Nowicki, Michal and Antosik, Pawel and Hulinska, Pavlina and Machatkova, Marie and Zabel, Maciej and Kempisty, Bartosz},
   article_location = {Prague},
   article_number = {6},
   doi = {http://dx.doi.org/10.17221/90/2016-CJAS},
   keywords = {pig; ovarian cycle; maturation; developmental competence; folliculogenesis},
   language = {eng},
   issn = {1212-1819},
   journal = {Czech Journal of Animal Science},
   title = {Expression of Genes Encoding Zona Pellucida Glycoproteins and Cortical Granule Distribution in Porcine Oocytes Isolated from Small and Medium Follicles in Relation to Puberty Status of Donors},
   volume = {62},
   year = {2017}
}

TY  - JOUR
ID  - 1386376
AU  - Ješeta, Michal - Budna, Joanna - Kranc, Wieslawa - Hanulakova, Sarka - Bryja, Artur - Chachula, Adrian - Ciesiólka, Sylwia - Wojtanowicz-Markiewicz, Katarzyna - Bukowska, Dorota - Brussow, Klaus-Peter - Bruska, Malgorzata - Nowicki, Michal - Antosik, Pawel - Hulinska, Pavlina - Machatkova, Marie - Zabel, Maciej - Kempisty, Bartosz
PY  - 2017
TI  - Expression of Genes Encoding Zona Pellucida Glycoproteins and Cortical Granule Distribution in Porcine Oocytes Isolated from Small and Medium Follicles in Relation to Puberty Status of Donors
JF  - Czech Journal of Animal Science
VL  - 62
IS  - 6
SP  - 234-241
EP  - 234-241
PB  - Czech Academy Agricultural Sciences
SN  - 12121819
KW  - pig
KW  - ovarian cycle
KW  - maturation
KW  - developmental competence
KW  - folliculogenesis
N2  - The zona pellucida proteins belong to a group of proteins that regulate the processes of gamete recognition, interaction, and fusion, since they are recognized as primary and secondary sperm receptors. It is suggested that cortical granule distribution is significantly associated with the zona pellucida structure and membrane preparation to cortical and acrosome reaction. Therefore, this study investigated the zona pellucida marker gene expression (ZP2 and ZP4 protein) in relation to cortical granules distribution in oocytes and puberty status of donors. Oocytes were collected from adult cyclic sows (isolated from medium and small follicles) and juvenile gilts (isolated only from small follicles). The oocytes were examined by RT-qPCR and by confocal microscopy. The expression of genes for ZP2 and ZP4 protein in oocytes collected from small follicles from cycling sows was higher in comparison to oocytes from medium follicles or oocytes collected from small follicles from juvenile gilts (P < 0.001). We also observed increased expression of both ZP2 and ZP4 mRNA in oocytes collected from small follicles (juvenile gilts) as compared to medium follicles (cycling sows) (P < 0.001). Moreover, we found a difference in the distribution of cortical granules. In oocytes from medium follicles, the peripheral localization of cortical granules was twice higher than their central concentration. However, in oocytes derived from small follicles (cyclic sows) cortical granules in comparison to oocytes from medium follicles were more centrally localized (P < 0.05). It has been suggested that the donor puberty status and the size of follicles significantly influenced the zona pellucida gene expression and cortical granule localization in porcine oocytes. This is accompanied by fertilization specificity and fertilizability of porcine oocytes in vitro.
ER  -

JEŠETA, Michal, Joanna BUDNA, Wieslawa KRANC, Sarka HANULAKOVA, Artur BRYJA, Adrian CHACHULA, Sylwia CIESIÓLKA, Katarzyna WOJTANOWICZ-MARKIEWICZ, Dorota BUKOWSKA, Klaus-Peter BRUSSOW, Malgorzata BRUSKA, Michal NOWICKI, Pawel ANTOSIK, Pavlina HULINSKA, Marie MACHATKOVA, Maciej ZABEL a Bartosz KEMPISTY. Expression of Genes Encoding Zona Pellucida Glycoproteins and Cortical Granule Distribution in Porcine Oocytes Isolated from Small and Medium Follicles in Relation to Puberty Status of Donors. \textit{Czech Journal of Animal Science}. Prague: Czech Academy Agricultural Sciences, 2017, roč.~62, č.~6, s.~234-241. ISSN~1212-1819. Dostupné z: https://dx.doi.org/10.17221/90/2016-CJAS.

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