Microscopic techniques in the study of African parasitic nematodes

HODOVÁ, Eliška, Šárka MAŠOVÁ, Kristýna ROSÍKOVÁ, Maarten Pieterjan VANHOVE and Iveta HODOVÁ. Microscopic techniques in the study of African parasitic nematodes. In Mikroskopie 2017. 2017.

Basic information

• Original name: Microscopic techniques in the study of African parasitic nematodes
• Authors: HODOVÁ, Eliška (203 Czech Republic, belonging to the institution), Šárka MAŠOVÁ (203 Czech Republic, belonging to the institution), Kristýna ROSÍKOVÁ (203 Czech Republic), Maarten Pieterjan VANHOVE (56 Belgium, belonging to the institution) and Iveta HODOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Mikroskopie 2017, 2017.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Slovakia
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/17:00094883
• Organization unit: Faculty of Science
• Keywords in English: Nematodes; Scanning electron microscopy; Lake Tanganyika

Abstract

Based on light and scanning electron microscopic studies, nematodes of the subgenus Procamallanus (Spirocamallanus) have been recorded in the intestine of six host cichlid species (Cyprichromis microlepidotus, Boulengerochromis microlepsis, Eretmodus marksmithi, Neolamprologus mondabu, Bathybates graueri, Oreochromis niloticus, Trematocara unimaculatum) were examined. Hosts were collected during a survey of fish parasites in the Lake Tanganyika (lakeside of Burundi). Obtained nematodes (glycerine-cleared) were examined by the Olympus BX51 light microscope (LM) equipped with differential interference contrast. They were measured and documented (drawings and micrographs) with all important signs, like buccal capsule, nerve ring, excretory pore, papillae, deirids, tail; males spiculae and females vulva. Scanning electron microscopy (SEM) was used for study of surface structures (cuticle ornamentation, number and distribution of papillae, deirids, amphids), for interior view to the capsule with ornamentation and for protruded males spiculae. The samples were examined using TESCAN MIRA3 FEG-SEM at an accelerating voltage of 5 kV. Based on our obtained results from LM and SEM we assume the presence of new species. Obtained results will be part of a High School Scientific Activity (SOČ) of Eliška Hodová (Gymnasium Brno – Řečkovice) “Parasitic nematodes from cichlids of the Lake Tanganyika“.

Links

• GBP505/12/G112, research and development project: Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation