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@proceedings{1387327,
author = {Reslová, Nikol and Škorpíková, Lucie and Snížková, Karolína and Kašný, Martin and Králík, Petr},
booktitle = {23rd Helminthological Days},
keywords = {xMAP; magnetic microspheres; parasites; molecular detection; diagnosis},
language = {eng},
isbn = {978-80-968473-8-9},
title = {xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS},
url = {http://pau.saske.sk/helminthdays2017/},
year = {2017}
}
TY - CONF
ID - 1387327
AU - Reslová, Nikol - Škorpíková, Lucie - Snížková, Karolína - Kašný, Martin - Králík, Petr
PY - 2017
TI - xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS
SN - 9788096847389
KW - xMAP
KW - magnetic microspheres
KW - parasites
KW - molecular detection
KW - diagnosis
UR - http://pau.saske.sk/helminthdays2017/
N2 - Parasitic zoonoses are recorded worldwide and some of them have endemic character. Parasitic agents may pass from animals to humans in several ways, e.g., by direct contact, vector, consumption of raw or undercooked foodstuffs containing the infective stages or by infective stages released into environment. Although in the last decades a number of novel diagnostic methodological approaches have been developed, the current diagnosis of some parasitic diseases is still based only on a combination of clinical signs, anamnesis, and direct visual identification of parasitological objects. The most common conventional diagnostic methods, such as microscopic examination, biochemical assays, ELISA or PCR, are available, but they are laborious, time-consuming and in many cases not reliable. With regard to the fact that parasites might exhibit very strictly confined localization within the host’s body – intracellular/extracellular or tissue/organ, sampling can be very problematic and it often leads to a false negative results. Therefore, there is niche for the development of more sensitive diagnostic assays. Microsphere-based assays utilizing xMAP technology are applicable for high-throughput, multiplex and simultaneous detection of different analytes within a single complex sample. xMAP multiplex assays are currently available in various nucleic acid and immunoassay formats, enabling simultaneous detection and typing of pathogenic agents and also antigen or antibody interception. As an open architecture platform, the xMAP technology is beneficial to end users and therefore it is used in various pharmaceutical, clinical and research laboratories. The aim of our research is to exploit a potential of this approach to develop a multiplex oligonucleotide ligation PCR assay (MOL-PCR) determined for detection of nucleic acids in diagnosis of intestinal parasites in areas where co-infections are common and to upgrade conventional singleplex ELISA into the multiplex level.
ER -
RESLOVÁ, Nikol, Lucie ŠKORPÍKOVÁ, Karolína SNÍŽKOVÁ, Martin KAŠNÝ a Petr KRÁLÍK. xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS. In \textit{23rd Helminthological Days}. 2017. ISBN~978-80-968473-8-9.
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