Ascorbic acid protects Coccomyxa subellipsoidea against metal
toxicity through modulation of ROS/NO balance and metal uptake

J 2017

Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake

KOVÁČIK, Jozef, Gabriela ROTKOVÁ, Marek BUJDOŠ, Petr BABULA, Viera PETERKOVÁ et. al.

Základní údaje

Originální název

Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake

Autoři

KOVÁČIK, Jozef (703 Slovensko), Gabriela ROTKOVÁ (203 Česká republika, garant, domácí), Marek BUJDOŠ (703 Slovensko), Petr BABULA (203 Česká republika, domácí), Viera PETERKOVÁ (703 Slovensko) a Peter MATÚŠ (703 Slovensko)

Vydání

JOURNAL OF HAZARDOUS MATERIALS, Amsterdam, Elsevier Science, 2017, 0304-3894

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30304 Public and environmental health

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 6.434

Kód RIV

RIV/00216224:14310/17:00097366

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.jhazmat.2017.06.035

UT WoS

000407188200021

Klíčová slova anglicky

Antioxidants; Fluorescence microscopy; Heavy metals; Oxidative stress; RT-PCR

Štítky

NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 3. 2018 16:28, Ing. Nicole Zrilić

Anotace

V originále

Impact of exogenous ascorbic acid (AsA, 100 uM) on acute metal toxicity (Pb and Hg, 24 h of exposure to 100 uM) in unicellular green alga Coccomyxa subellipsoidea was studied. Hg (often extensively) depleted amount of pigments, potassium, soluble proteins, endogenous AsA, non-protein thiols and nitric oxide signal but elevated ROS signal and activities of catalase and superoxide dismutase. Responses to Pb application (if any) were less visible with ROS signal being slightly elevated and amount of non-protein thiols being slightly reduced. Exogenous AsA considerably improved mainly Hg-induced damage at the level of oxidative stress and physiological parameters and evoked an increase in nitric oxide signal. Maximum amount of total Hg was almost 4-fold higher than that of Pb (36.5 vs. 9.73 mg/g DW) and AsA depleted both total and intracellular accumulation. At the level of ascorbate-related proteins, exogenous AsA suppressed metal-induced expression and activity of MDHAR and APX but not of DHAR, which may indicate a role of DHAR in algal stress tolerance. Our data suggest that given Coccomyxa species is suitable candidate for remediation of Hg or Pb and that ascorbic acid effectively ameliorates metal-induced toxicity without side effects.

Citovat

KOVÁČIK, Jozef, Gabriela ROTKOVÁ, Marek BUJDOŠ, Petr BABULA, Viera PETERKOVÁ a Peter MATÚŠ. Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake. JOURNAL OF HAZARDOUS MATERIALS. Amsterdam: Elsevier Science, 2017, roč. 339, October, s. 200-207. ISSN 0304-3894. Dostupné z: https://dx.doi.org/10.1016/j.jhazmat.2017.06.035.

@article{1387611,
   author = {Kováčik, Jozef and Rotková, Gabriela and Bujdoš, Marek and Babula, Petr and Peterková, Viera and Matúš, Peter},
   article_location = {Amsterdam},
   article_number = {October},
   doi = {http://dx.doi.org/10.1016/j.jhazmat.2017.06.035},
   keywords = {Antioxidants; Fluorescence microscopy; Heavy metals; Oxidative stress; RT-PCR},
   language = {eng},
   issn = {0304-3894},
   journal = {JOURNAL OF HAZARDOUS MATERIALS},
   title = {Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake},
   volume = {339},
   year = {2017}
}

TY  - JOUR
ID  - 1387611
AU  - Kováčik, Jozef - Rotková, Gabriela - Bujdoš, Marek - Babula, Petr - Peterková, Viera - Matúš, Peter
PY  - 2017
TI  - Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake
JF  - JOURNAL OF HAZARDOUS MATERIALS
VL  - 339
IS  - October
SP  - 200-207
EP  - 200-207
PB  - Elsevier Science
SN  - 03043894
KW  - Antioxidants
KW  - Fluorescence microscopy
KW  - Heavy metals
KW  - Oxidative stress
KW  - RT-PCR
N2  - Impact of exogenous ascorbic acid (AsA, 100 uM) on acute metal toxicity (Pb and Hg, 24 h of exposure to 100 uM) in unicellular green alga Coccomyxa subellipsoidea was studied. Hg (often extensively) depleted amount of pigments, potassium, soluble proteins, endogenous AsA, non-protein thiols and nitric oxide signal but elevated ROS signal and activities of catalase and superoxide dismutase. Responses to Pb application (if any) were less visible with ROS signal being slightly elevated and amount of non-protein thiols being slightly reduced. Exogenous AsA considerably improved mainly Hg-induced damage at the level of oxidative stress and physiological parameters and evoked an increase in nitric oxide signal. Maximum amount of total Hg was almost 4-fold higher than that of Pb (36.5 vs. 9.73 mg/g DW) and AsA depleted both total and intracellular accumulation. At the level of ascorbate-related proteins, exogenous AsA suppressed metal-induced expression and activity of MDHAR and APX but not of DHAR, which may indicate a role of DHAR in algal stress tolerance. Our data suggest that given Coccomyxa species is suitable candidate for remediation of Hg or Pb and that ascorbic acid effectively ameliorates metal-induced toxicity without side effects.
ER  -

KOVÁČIK, Jozef, Gabriela ROTKOVÁ, Marek BUJDOŠ, Petr BABULA, Viera PETERKOVÁ a Peter MATÚŠ. Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake. \textit{JOURNAL OF HAZARDOUS MATERIALS}. Amsterdam: Elsevier Science, 2017, roč.~339, October, s.~200-207. ISSN~0304-3894. Dostupné z: https://dx.doi.org/10.1016/j.jhazmat.2017.06.035.

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